1233049
doi
10.1038/320367a0
oai:zenodo.org:1233049
Wong-Staal, Flossie
Feinberg, Mark B.
Josephs, Steven F.
Harper, Mary E.
Marselle, Lisa M.
Reyes, Gregory
Gonda, Matthew A.
Aldovini, Anna
Debouk, Christine
Gallo, Robert C.
Fisher, A.G. et al. The trans-activator gene of HTLV-III is essential for virus replication. Nature 320, 367−371
Fisher, Amanda G.
info:eu-repo/semantics/openAccess
Creative Commons Zero v1.0 Universal
https://creativecommons.org/publicdomain/zero/1.0/legalcode
Studies of the genomic structure of human T-lymphotropic virus type III (HTLV-III) and related viruses, implicated as the causal agent of acquired immune deficiency syndrome (AIDS), have identified a sixth open reading frame in addition to the five previously known within the genome (gag, pol, sor, env and 3'orf). This gene, called tat-III, lies between the sor and env genes and is able to mediate activation, in a trans configuration, of the genes linked to HTLV-III long terminal repeat (LTR) sequences. We now present evidence that the product of tat-III is an absolute requirement for virus expression. We show that derivatives of a biologically competent molecular clone of HTLV-III, in which the tat-III gene is deleted or the normal splicing abrogated, failed to produce or expressed unusually low levels of virus, respectively, when transfected into T-cell cultures. The capacity of these tat-III-defective genomes was transiently restored by co-transfection of a plasmid clone containing a functional tat-III gene or by introducing the TAT-III protein itself. As HTLV-III and related viruses are the presumed causal agents of AIDS and associated conditions, the observation that tat-III is critical for HTLV-III replication has important clinical implications, and suggests that specific inhibition of the activity of tat-III could be a novel and effective therapeutic approach to the treatment of AIDS.
Zenodo
1986-03-27
info:eu-repo/semantics/article
1233048
1579537715.072147
727723
md5:194f670f8dba84793aaf8285d24188e3
https://zenodo.org/records/1233049/files/article.pdf
public