1232816
doi
10.1007/s10974-004-6060-7
oai:zenodo.org:1232816
Straight, Aaron F.
Mitchison, Timothy
Sellers, James R.
Specificity of blebbistatin, an inhibitor of myosin II
Limouze, John
info:eu-repo/semantics/openAccess
Creative Commons Zero v1.0 Universal
https://creativecommons.org/publicdomain/zero/1.0/legalcode
Blebbistatin is a small molecule inhibitor discovered in a screen for inhibitors of nonmuscle myosin IIA. We have examined the specificity and potency of the drug by assaying its effects on the actin-activated MgATPase assay of diverse members of the myosin superfamily. Blebbistatin potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC50 values ranging from 0.5 to 5 microM. Interestingly, smooth muscle which is highly homologous to vertebrate nonmuscle myosin is only poorly inhibited (IC50=80 microM). The drug potently inhibits Dictyostelium myosin II, but poorly inhibits Acanthamoeba myosin II. Blebbistatin did not inhibit representative myosin superfamily members from classes I, V, and X.
Zenodo
2004-02-01
info:eu-repo/semantics/article
1232815
1579540644.402997
188376
md5:b485805c64975252e7dc4226e5228133
https://zenodo.org/records/1232816/files/article.pdf
public