10.1006/geno.1996.0540
https://zenodo.org/records/1229705
oai:zenodo.org:1229705
Shen, Zhiyuan
Zhiyuan
Shen
Pardington-Purtymun, Paige E.
Paige E.
Pardington-Purtymun
Comeaux, Jarmon C.
Jarmon C.
Comeaux
Moyzis, Robert K.
Robert K.
Moyzis
Chen, David J.
David J.
Chen
Associations of UBE2I with RAD52, UBL1, p53, and RAD51 Proteins in a Yeast Two-Hybrid System
Zenodo
1996
1996-10-01
Creative Commons Zero v1.0 Universal
The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the "bait" in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52's self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53-mediated pathway(s), and ubiquitination interact through human UBE2I.