Journal article Open Access

COMPARISON OF HCG VS GNRH EFFECTS IN DOUBLE OVSYNCH ON FIRST-SERVICE CONCEPTION RATES IN ANESTRUS DAIRY COWS

Naglis, Gundars; Fasulkov, Ivan; Karadaev, Manol; Vasileva, Radina; Petrovas, Georgios; Vasilev, Nasko

The aim of the present study was to compare first-service conception rates after inclusion of either hCG or GnRH in Double Ovsynch to anestrous dairy cows. The animals with follicle size ≥10 mm and no corpus luteum in ovaries were allotted into 2 groups. At the start of the programme, follicle sizes were 13.7±1.5 mm and 13.9±1.2 mm respectively (р=0.5). Higher percentage of ovulations (72.7%) were established in cows treated with hCG vs those treated with GnRH (64.4%, р<0.2094). Follicle sizes at the time of AI were 15.9±0.5 mm and 16.2±0.4 mm (р=0.5). Pregnancy was detected in 38.6% of cows treated with hCG and 42.2% of cows treated with GnRH (р<0.3501)

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  • Ayres H., Ferreira R. M., Cunha A. P., Araújo R. R., Wiltbank M. C. (2013). Double- Ovsynch in high-producing dairy cows: effects on progesterone concentrations and ovulation to GnRH treatments. Theriogenology 2013: Vol. 79: 159–164.
  • Bello N. M., Steibel J. P., Pursley R. J. (2006). Optimizing ovulation to first GnRH improved outcomes to each hormonal injection of Ovsynch in lactating dairy cows. J Dairy Sci 2006: Vol. 89: 3413–24.
  • Bisinotto R. S., Chebel R. C., Santos JEP. (2010). Follicular wave of the ovulatory follicle and not cyclic status influences fertility of dairy cows. Journal of Dairy Science 2010: Vol. 93: 3578–3587.
  • Bisinotto R. S., Ribeiro E. S., Lima F. S., Martinez N., Greco L. F., Barbosa L. F., Bueno P. P., Scagion L. F., Thatcher W. W., Santos JEP. (2013). Targeted progesterone supplementation improves fertility in lactating dairy cows without a corpus luteum at the initiation of the timed artificial insemination protocol. Journal of Dairy Science 2013: Vol. 96: 2214–2225.
  • Bisinotto R. S., Santos JEP. (2012). The use of endocrine treatments to improve pregnancy rates in cattle. Reproduction Fertility and Development 2012: Vol. 24: 258–266.
  • Chebel R. C., Santos J. E. P., Cerri R. L. A., Rutigliano H. M., Bruno R. G. S. (2006). Reproduction in dairy cows following progesterone insert presynchronization and resynchronization protocols. J Dairy Sci 2006: Vol. 89: 4205–19.
  • Chebel R. C., Santos JEP. (2010). Effect of inseminating cows in estrus following a presynchronization protocol on reproductive and lactation performances. Journal of Dairy Science 2010: Vol. 93: 4632–4643.
  • El-Zarkouny S. Z., Cartmill J. A., Hensley B. A., Stevenson J. S. (2004). Pregnancy in dairy cows after synchronized ovulation regimens with or without presynchronization and progesterone. J Dairy Sci 2004: Vol. 87: 1024–37.
  • Giordano J. O., Wiltbank M. C., Fricke P. M., Bas S., Pawlisch R., Guenther J., Nascimento A. B. (2013). Effect of increasing GnRH and PGF2α dose during double- Ovsynch on ovulatory response, luteal regression, and fertility of lactating dairy cows. Theriogenology 2013: Vol. 80: 773–783
  • Gumen A., Guenther J. N., Wiltbank M. C. (2003). Follicular size and response to Ovsynch versus detection of estrus in anovular and ovular lactating dairy cows. J Dairy Sci 2003: 86: 3184–94
  • Gumen A., Souza A. H., Cunha A. P., Silva E., Guenther J. N., Wiltbank M. C. (2005). Effect of GnRH between pre-synch injections and estradiol-17b during the Ovsynch protocol on conception rates in lactating dairy cows. J Dairy Sci 2005: Vol. 88: 170 [Abstr.]
  • Keskin G., Yilmazbas-Mecitoglu, Gumen A., Karakaya E., Darici R., Okut H. (2010). Effect of hCG vs. GnRH at the beginning of the Ovsynch on first ovulation and conception rates in cyclic lactating dairy cows. Theriogenology 2010: Vol. 74: 602–607.
  • Lopes A. S., Butler S. T., Gilbert R. O., Butler W. R. (2007). Relationship of pre-ovulatory follicle size, estradiol concentrations and season to pregnancy outcome in dairy cows. Anim Reprod Sci 2007: Vol. 99: 34–43
  • Mihn M., Baguisi A., Boland M. P., Roche J. F. (1994). Association between the duration of dominance of the ovulatory follicle and pregnancy rate in beef heifers. J Reprod Fertil 1994: Vol. 102: 123–30.
  • Moreira F., Orlandi C., Risco C. A., Mattos R., Lopes F., Thatcher W. W. (2001). Effects of presynchronization and bovine somatotropin on pregnancy rates to timed artificial insemination protocol in lactating dairy cows. J Dairy Sci 2001: Vol. 84: 1646–59.
  • Navanukraw C., Redmer D. A., Reynolds L. P., Kirsch J. D., Grazul- Bilska A. T., Fricke P. M. (2004). A modified presynchronization protocol improves fertility to timed artificial insemination in lactating dairy cows. J Dairy Sci 2004: Vol. 87: 1551–7
  • Ozturk A., Cirit U., Baran A., Ak K. (2010). Is Doublesynch protocol a new alternative for timed artificial insemination in anestrous dairy cows. Theriogenology 2010: Vol. 73: 568–576
  • Perry G. A., Smith M. F., Lucy M. C., Green J. A., Parks T. E., MacNeilv M. D., Roberts A. J., Geary T. W. (2005). Relationship between follicle size at insemination and pregnancy succes. PNAS USA 2005: Vol. 102: 5268–73.
  • Pursley R. J., Kosorok M. R., Wiltbank M. C. (1997). Reproductive management of lactating dairy cows using synchronization of ovulation. J Dairy Sci 1997: Vol. 80: 301–6
  • Pursley R. J., Mee M. O., Wiltbank M. C. (1995). Synchronization of ovulation in dairy cows using PGF2a and GnRH. Theriogenology 1995: Vol. 44: 915–23
  • Revah I., Butler W. R. (1996). Prolonged dominance of follicles reduced viability of bovine oocytes. J Reprod Fertil 1996: 106: 39–47
  • Ribeiro E. S., Bisinotto R. S., Favoreto M. G., Martins L. T., Cerri RLA., Silvestre F. T., Greco L. F., Thatcher W. W., Santos JEP. (2012a). Fertility in dairy cows following presynchronization and administering twice the luteolytic dose of prostaglandin F2α as one or two injections in the 5-day timed artificial insemination protocol. Theriogenology 2012a: Vol. 78: 273–284
  • Ribeiro E. S., Monteiro A. P., Lima F. S., Ayres H., Bisinotto R. S., Favoreto M., Greco L. F., Marsola R. S., Thatcher W. W., Santos JEP. (2012b). Effects of presynchronization and length of proestrus on fertility of grazing dairy cows subjected to a 5-day timed artificial insemination protocol. Journal of Dairy Science 2012b: Vol. 95: 2513–2522
  • Santos JEP, Rutigliano H. M., Sá Filho M. F. (2009). Risk factors for resumption of postpartum estrous cycles and embryonic survival in lactating dairy cows. Animal Reproduction Science 2009: Vol. 110: 207–221.
  • Silva E., Sterry R. A., Fricke P. M. (2007). Assessment of a practical method for identifying anovular dairy cows synchronized for first postpartum timed artificial insemination. J Dairy Sci 2007: Vol. 90: 3255–62
  • Souza A. H., Gumen A., Silva EPB, Cunha A. P., Guenther J. N., Peto C. M. (2007). Supplementation with estradiol-17b before the last gonadotropin-releasing hormone injection of the Ovsynch protocol in lactation dairy cows. J Dairy Sci 2007: Vol. 90: 4623–34
  • Souza, A. H., Ayres H., Ferreira R. M, Wiltbank M. C. (2008). A new presynchronization system (Double-Ovsynch) increases fertility at first postpartum timed AI in lactating dairy cows. Theriogenology 2008: Vol. 70: 208–215.
  • Vasconcelos J. L., Silcox R. W., Rosa G. J., Pursley R. J., Wiltbank M. C. (1999). Synchronization rate, size of the ovulatory follicle, and pregnancy rate after synchronization of ovulation beginning on different days of the estrous cycle in lactating dairy cows. Theriogenology 1999: Vol. 52: 1067– 78
  • Walsh R. B., Kelton D. F., Duffield T. F., Leslie K. E., Walton J. S., LeBlanc S. J. (2007). Prevalence and risk factors for postpartum anovulatory condition in dairy cows. Journal of Dairy Science 2007: Vol. 90: 315–324
  • Wiltbank M. C., Pursley J. R. (2014). The cow as an induced ovulator: timed AI after synchronization of ovulation. Theriogenology 2014: Vol. 81: 170–185.
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