Journal article Open Access

In vitro single and combined mycotoxins degradation by Ery4 laccase from Pleurotus eryngii and redox mediators

Martina Loi; Francesca Fanelli; Maria Teresa Cimmarusti; Valentina Mirabelli; Miriam Haidukowski; Antonio F.Logrieco; RoccoCaliandro; GiuseppinaMule


MARC21 XML Export

<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
  <leader>00000nam##2200000uu#4500</leader>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Mycotoxins Bioremediation Laccase-Mediator System Multi-mycotoxin degradation</subfield>
  </datafield>
  <controlfield tag="005">20190409133918.0</controlfield>
  <controlfield tag="001">1216974</controlfield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">709620</subfield>
    <subfield code="z">md5:19f7eddb17cfc5760d0372a44960e4a9</subfield>
    <subfield code="u">https://zenodo.org/record/1216974/files/1-s2.0-S0956713518300781-main.pdf</subfield>
  </datafield>
  <datafield tag="542" ind1=" " ind2=" ">
    <subfield code="l">open</subfield>
  </datafield>
  <datafield tag="260" ind1=" " ind2=" ">
    <subfield code="c">2018-03-14</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="O">
    <subfield code="p">openaire</subfield>
    <subfield code="o">oai:zenodo.org:1216974</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="4">
    <subfield code="p">Food Control</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="u">a     Institute of Sciences of Food Production, National Research Council, Via Amendola 122/O, Bari 70126, Italy  b     Department of Economics, University of Foggia, Via Napoli 25, Foggia 71122, Italy  c     Institute of Crystallography, National Research Council, Via Amendola 122/O, Bari 70126, Italy</subfield>
    <subfield code="a">Martina Loi; Francesca Fanelli; Maria Teresa Cimmarusti; Valentina Mirabelli; Miriam Haidukowski; Antonio F.Logrieco; RoccoCaliandro; GiuseppinaMule</subfield>
  </datafield>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">In vitro single and combined mycotoxins degradation by Ery4 laccase from Pleurotus eryngii and redox mediators</subfield>
  </datafield>
  <datafield tag="536" ind1=" " ind2=" ">
    <subfield code="c">678781</subfield>
    <subfield code="a">Integrated and innovative key actions for mycotoxin management in the food and feed chain</subfield>
  </datafield>
  <datafield tag="540" ind1=" " ind2=" ">
    <subfield code="u">http://creativecommons.org/licenses/by/4.0/legalcode</subfield>
    <subfield code="a">Creative Commons Attribution 4.0 International</subfield>
  </datafield>
  <datafield tag="650" ind1="1" ind2="7">
    <subfield code="a">cc-by</subfield>
    <subfield code="2">opendefinition.org</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">&lt;p&gt;Abstract&lt;/p&gt;

&lt;p&gt;&lt;a href="https://www.sciencedirect.com/topics/food-science/mycotoxin"&gt;Mycotoxin&lt;/a&gt; contamination of staple &lt;a href="https://www.sciencedirect.com/topics/food-science/food-commodity"&gt;food commodities&lt;/a&gt; is a relevant health and economic issue worldwide. The development of green and effective reduction strategies to counteract the contamination by multiple mycotoxins has become an urgent need. The aim of this work was to evaluate the capability of a laccase (LC) from &lt;em&gt;Pleurotus eryngii&lt;/em&gt; and a laccase-mediator systems (LMSs) to degrade aflatoxin B&lt;sub&gt;1&lt;/sub&gt; (AFB&lt;sub&gt;1&lt;/sub&gt;), fumonisin B&lt;sub&gt;1&lt;/sub&gt; (FB&lt;sub&gt;1&lt;/sub&gt;), ochratoxin A (OTA), deoxynivalenol (DON), Zearalenone (ZEN) and T-2 toxin in &lt;em&gt;in&amp;nbsp;vitro&lt;/em&gt; assays. In addition, the simultaneous mycotoxin degradation capability with selected LMSs was evaluated with combinations of AFB&lt;sub&gt;1&lt;/sub&gt; and ZEN, and FB&lt;sub&gt;1&lt;/sub&gt; and T-2 toxin. Redox mediators were found to drastically increasethe degradation efficiencies of the enzyme. AFB&lt;sub&gt;1&lt;/sub&gt;, FB&lt;sub&gt;1&lt;/sub&gt;, OTA, ZEN and T-2 toxin degradation by the best performing LMS were 73%, 74%, 27%, 100% and 40%, respectively. No degradation was registered for DON. Notably, AFB&lt;sub&gt;1&lt;/sub&gt; and ZEN were simultaneously degraded by 86% and 100%, while FB&lt;sub&gt;1&lt;/sub&gt; and T-2 by 25% and 100%, respectively. LMS proved to be a promising approach to enhance degradation properties of LC enzymes and for the potential development of a multi-mycotoxin reducing method.&lt;/p&gt;</subfield>
  </datafield>
  <datafield tag="024" ind1=" " ind2=" ">
    <subfield code="a">10.1016/j.foodcont.2018.02.032</subfield>
    <subfield code="2">doi</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">publication</subfield>
    <subfield code="b">article</subfield>
  </datafield>
</record>
32
41
views
downloads
Views 32
Downloads 41
Data volume 29.1 MB
Unique views 32
Unique downloads 39

Share

Cite as