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Abstract
\n\nMycotoxin contamination of staple food commodities is a relevant health and economic issue worldwide. The development of green and effective reduction strategies to counteract the contamination by multiple mycotoxins has become an urgent need. The aim of this work was to evaluate the capability of a laccase (LC) from Pleurotus eryngii and a laccase-mediator systems (LMSs) to degrade aflatoxin B1 (AFB1), fumonisin B1 (FB1), ochratoxin A (OTA), deoxynivalenol (DON), Zearalenone (ZEN) and T-2 toxin in in vitro assays. In addition, the simultaneous mycotoxin degradation capability with selected LMSs was evaluated with combinations of AFB1 and ZEN, and FB1 and T-2 toxin. Redox mediators were found to drastically increasethe degradation efficiencies of the enzyme. AFB1, FB1, OTA, ZEN and T-2 toxin degradation by the best performing LMS were 73%, 74%, 27%, 100% and 40%, respectively. No degradation was registered for DON. Notably, AFB1 and ZEN were simultaneously degraded by 86% and 100%, while FB1 and T-2 by 25% and 100%, respectively. LMS proved to be a promising approach to enhance degradation properties of LC enzymes and for the potential development of a multi-mycotoxin reducing method.
", "funding": [ { "award": { "acronym": "MycoKey", "id": "00k4n6c32::678781", "identifiers": [ { "identifier": "https://cordis.europa.eu/projects/678781", "scheme": "url" } ], "number": "678781", "program": "H2020", "title": { "en": "Integrated and innovative key actions for mycotoxin management in the food and feed chain" } }, "funder": { "id": "00k4n6c32", "name": "European Commission" } } ], "publication_date": "2018-03-14", "publisher": "Zenodo", "resource_type": { "id": "publication-article", "title": { "de": "Zeitschriftenartikel", "en": "Journal article" } }, "rights": [ { "description": { "en": "The Creative Commons Attribution license allows re-distribution and re-use of a licensed work on the condition that the creator is appropriately credited." }, "icon": "cc-by-icon", "id": "cc-by-4.0", "props": { "scheme": "spdx", "url": "https://creativecommons.org/licenses/by/4.0/legalcode" }, "title": { "en": "Creative Commons Attribution 4.0 International" } } ], "subjects": [ { "subject": "Mycotoxins Bioremediation Laccase-Mediator System Multi-mycotoxin degradation" } ], "title": "In vitro single and combined mycotoxins degradation by Ery4 laccase from Pleurotus eryngii and redox mediators" }, "parent": { "access": { "owned_by": { "user": 25066 } }, "communities": { "entries": [ { "access": { "member_policy": "open", "record_policy": "open", "review_policy": "open", "visibility": "public" }, "children": { "allow": false }, "created": "2022-11-23T15:53:29.436323+00:00", "custom_fields": {}, "deletion_status": { "is_deleted": false, "status": "P" }, "id": "f0a8b890-f97a-4eb2-9eac-8b8a712d3a6c", "links": {}, "metadata": { "curation_policy": "", "description": "", "page": "", "title": "EU" }, "revision_id": 0, "slug": "eu", "updated": "2022-11-23T15:53:29.436331+00:00" } ], "ids": [ "f0a8b890-f97a-4eb2-9eac-8b8a712d3a6c" ] }, "id": "1216973", "pids": {} }, "pids": { "doi": { "identifier": "10.1016/j.foodcont.2018.02.032", "provider": "external" }, "oai": { "identifier": "oai:zenodo.org:1216974", "provider": "oai" } }, "revision_id": 5, "stats": { "all_versions": { "data_volume": 116377680.0, "downloads": 164, "unique_downloads": 160, "unique_views": 135, "views": 149 }, "this_version": { "data_volume": 116377680.0, "downloads": 164, "unique_downloads": 160, "unique_views": 135, "views": 149 } }, "status": "published", "updated": "2020-01-20T17:44:22.948382+00:00", "versions": { "index": 1, "is_latest": true } }