10.5281/zenodo.1215836
https://zenodo.org/records/1215836
oai:zenodo.org:1215836
Lu, Huasong
Huasong
Lu
0000-0003-3002-7231
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720
Yu, Dan
Dan
Yu
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720
Hansen, Anders S.
Anders S.
Hansen
0000-0001-7540-7858
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720
Ganguly, Sourav
Sourav
Ganguly
0000-0003-1002-9002
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720
Liu, Rongdiao
Rongdiao
Liu
School of Pharmaceutical Sciences, Xiamen University, Xiamen, Fujian 361005, China
Heckert, Alec
Alec
Heckert
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720
Darzacq, Xavier
Xavier
Darzacq
0000-0003-2537-8395
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720
Zhou, Qiang
Qiang
Zhou
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720
Single particle tracking data for "Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD"
Zenodo
2018
Single-particle tracking
Single molecule
Imaging
Spot-On
Cyclin T1
P-TEFb
RNA Polymerase II
2018-04-10
eng
10.5281/zenodo.1215835
Creative Commons Attribution 4.0 International
Experimental single-particle tracking (SPT) data supporting "Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD"
This dataset contains all the raw SPT data reported in "Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD" in the form of SPT trajectories. The SPT trajectories are provided in two different formats for convenience: a CSV format and a Matlab format. Both formats are readable by Spot-On: https://spoton.berkeley.edu/
The SPT data contains "fast tracking" spaSPT data (Figure 2d) and this data was analyzed using the Matlab version of Spot-On which can be found and downloaded at: https://gitlab.com/tjian-darzacq-lab/spot-on-matlab
The SPT data also contains "slow tracking" SPT data (Figure 2e).
Full details about the Matlab and CSV formats are provided in the ReadMe files in the associated zip files.
Please see the associated manuscript for a detailed description of how the data was acquired and analyzed. For questions about the data please contact Anders Sejr Hansen at anders.sejr.hansen {at} berkeley {dot} edu.
This work was supported by the Public Health Service grant R01AI041757 from the National Institutes of Health to Q.Z; the California Institute of Regenerative Medicine grant LA1-08013 and, National Institutes of Health grants UO1-EB021236 and U54-DK107980 to X.D.; The Siebel Stem Cell Institute to A.S.H.