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Single particle tracking data for "Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD"

Lu, Huasong; Yu, Dan; Hansen, Anders S.; Ganguly, Sourav; Liu, Rongdiao; Heckert, Alec; Darzacq, Xavier; Zhou, Qiang


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  <identifier identifierType="DOI">10.5281/zenodo.1215836</identifier>
  <creators>
    <creator>
      <creatorName>Lu, Huasong</creatorName>
      <givenName>Huasong</givenName>
      <familyName>Lu</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-3002-7231</nameIdentifier>
      <affiliation>Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720</affiliation>
    </creator>
    <creator>
      <creatorName>Yu, Dan</creatorName>
      <givenName>Dan</givenName>
      <familyName>Yu</familyName>
      <affiliation>Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720</affiliation>
    </creator>
    <creator>
      <creatorName>Hansen, Anders S.</creatorName>
      <givenName>Anders S.</givenName>
      <familyName>Hansen</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0001-7540-7858</nameIdentifier>
      <affiliation>Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720</affiliation>
    </creator>
    <creator>
      <creatorName>Ganguly, Sourav</creatorName>
      <givenName>Sourav</givenName>
      <familyName>Ganguly</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-1002-9002</nameIdentifier>
      <affiliation>Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720</affiliation>
    </creator>
    <creator>
      <creatorName>Liu, Rongdiao</creatorName>
      <givenName>Rongdiao</givenName>
      <familyName>Liu</familyName>
      <affiliation>School of Pharmaceutical Sciences, Xiamen University, Xiamen, Fujian 361005, China</affiliation>
    </creator>
    <creator>
      <creatorName>Heckert, Alec</creatorName>
      <givenName>Alec</givenName>
      <familyName>Heckert</familyName>
      <affiliation>Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720</affiliation>
    </creator>
    <creator>
      <creatorName>Darzacq, Xavier</creatorName>
      <givenName>Xavier</givenName>
      <familyName>Darzacq</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-2537-8395</nameIdentifier>
      <affiliation>Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720</affiliation>
    </creator>
    <creator>
      <creatorName>Zhou, Qiang</creatorName>
      <givenName>Qiang</givenName>
      <familyName>Zhou</familyName>
      <affiliation>Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Single particle tracking data for "Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD"</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2018</publicationYear>
  <subjects>
    <subject>Single-particle tracking</subject>
    <subject>Single molecule</subject>
    <subject>Imaging</subject>
    <subject>Spot-On</subject>
    <subject>Cyclin T1</subject>
    <subject>P-TEFb</subject>
    <subject>RNA Polymerase II</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2018-04-10</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Dataset"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/1215836</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.1215835</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/zenodo</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;&lt;strong&gt;Experimental single-particle tracking (SPT) data supporting &amp;quot;Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD&amp;quot;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;This dataset contains all the raw SPT data reported in &amp;quot;Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD&amp;quot; in the form of SPT trajectories. The SPT trajectories are provided in two different formats for convenience: a CSV format and a Matlab format. Both formats are readable by Spot-On: https://spoton.berkeley.edu/&lt;/p&gt;

&lt;p&gt;The SPT data contains &amp;quot;fast tracking&amp;quot; spaSPT data (Figure 2d) and this data was analyzed using the Matlab version of Spot-On which can be found and downloaded at: https://gitlab.com/tjian-darzacq-lab/spot-on-matlab&lt;/p&gt;

&lt;p&gt;The SPT data also contains &amp;quot;slow tracking&amp;quot; SPT data (Figure 2e).&lt;/p&gt;

&lt;p&gt;Full details about the Matlab and CSV formats are provided in the ReadMe files in the associated zip files.&lt;/p&gt;

&lt;p&gt;Please see the associated manuscript for a detailed description of how the data was acquired and analyzed. For questions about the data please contact Anders Sejr Hansen at anders.sejr.hansen {at} berkeley {dot} edu.&lt;/p&gt;</description>
    <description descriptionType="Other">This work was supported by the Public Health Service grant R01AI041757 from the National Institutes of Health to Q.Z; the California Institute of Regenerative Medicine grant LA1-08013 and, National Institutes of Health grants UO1-EB021236 and U54-DK107980 to X.D.; The Siebel Stem Cell Institute to A.S.H.</description>
  </descriptions>
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