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Single particle tracking data for "Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD"

Lu, Huasong; Yu, Dan; Hansen, Anders S.; Ganguly, Sourav; Liu, Rongdiao; Heckert, Alec; Darzacq, Xavier; Zhou, Qiang

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    <dct:title>Single particle tracking data for "Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD"</dct:title>
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    <dcat:keyword>Single molecule</dcat:keyword>
    <dcat:keyword>Imaging</dcat:keyword>
    <dcat:keyword>Spot-On</dcat:keyword>
    <dcat:keyword>Cyclin T1</dcat:keyword>
    <dcat:keyword>P-TEFb</dcat:keyword>
    <dcat:keyword>RNA Polymerase II</dcat:keyword>
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    <dct:description>&lt;p&gt;&lt;strong&gt;Experimental single-particle tracking (SPT) data supporting &amp;quot;Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD&amp;quot;&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;This dataset contains all the raw SPT data reported in &amp;quot;Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD&amp;quot; in the form of SPT trajectories. The SPT trajectories are provided in two different formats for convenience: a CSV format and a Matlab format. Both formats are readable by Spot-On: https://spoton.berkeley.edu/&lt;/p&gt; &lt;p&gt;The SPT data contains &amp;quot;fast tracking&amp;quot; spaSPT data (Figure 2d) and this data was analyzed using the Matlab version of Spot-On which can be found and downloaded at: https://gitlab.com/tjian-darzacq-lab/spot-on-matlab&lt;/p&gt; &lt;p&gt;The SPT data also contains &amp;quot;slow tracking&amp;quot; SPT data (Figure 2e).&lt;/p&gt; &lt;p&gt;Full details about the Matlab and CSV formats are provided in the ReadMe files in the associated zip files.&lt;/p&gt; &lt;p&gt;Please see the associated manuscript for a detailed description of how the data was acquired and analyzed. For questions about the data please contact Anders Sejr Hansen at anders.sejr.hansen {at} berkeley {dot} edu.&lt;/p&gt;</dct:description>
    <dct:description>This work was supported by the Public Health Service grant R01AI041757 from the National Institutes of Health to Q.Z; the California Institute of Regenerative Medicine grant LA1-08013 and, National Institutes of Health grants UO1-EB021236 and U54-DK107980 to X.D.; The Siebel Stem Cell Institute to A.S.H.</dct:description>
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Publication date:
April 10, 2018
DOI:
10.5281/zenodo.1215836

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Keyword(s):
Single-particle tracking Single molecule Imaging Spot-On Cyclin T1 P-TEFb RNA Polymerase II
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