Dataset Open Access

Br soaked lysozyme datasets collected with the mixed scheme implemented in HITO in the ZOO system

Kunio Hirata

Bromide soaked lysozyme crystals were used as standard crystals for evaluation of the automated data collection, ZOO, developed at SPring-8 BL32XU.

Crystals those sizes ranged 10-100 µm were cryocooled together in the same cryoloop and processed 'mixed scheme' implemented in the ZOO system.

Beam size : 10(H) x 15(V) µm2

After 2D raster scanning the loop, HITO detects 1 crystals for full rotation helical, 7 crystals for partial helical, and 62 crystals for multiple small wedge schemes, respectively. A full rotation helical, partial helical and small wedge datasets contain total oscillation of 60 deg, 40 deg and 5 deg. respectively. 

The crystals belong to space group P43212 with mean unit cell parameters a=b=79.1, c=37.5.

Data was processed up to 1.50 Å resolution in the published result (Hirata et al. in preparation) using KAMO.

<Available data>

  • # Beamline:     SPring-8 BL32XU
  • # wavelength    0.9000 A
  • # Detector :    EIGER X 9M
  • # ZOO mode: mixed scheme (HITO)
  • #####################################################
  • # Multiple small wedge datasets
  • # 'CPS1991-05-multi_master.h5' manages 62 datasets
  • #####################################################
  • # 5.0 deg. / datasets
  • # 0.1 deg. / frame
  • #  50 frames / datasets
  • # 100 frames in each data file
  • #####################################################
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000001.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000002.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000003.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000004.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000005.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000006.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000007.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000008.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000009.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000010.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000011.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000012.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000013.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000014.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000015.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000016.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000017.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000018.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000019.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000020.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000021.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000022.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000023.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000024.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000025.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000026.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000027.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000028.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000029.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000030.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_data_000031.h5
  • hito-CPS1991-05//data/CPS1991-05-multi_master.h5

 

  • #####################################################
  • # 'cry-00-??_master.h5' are for 'partial helical' datasets
  • #####################################################
  • # 40.0 deg. / datasets
  • #  0.1 deg. / frame
  • #  400 frames / datasets
  • #  100 frames in each data file
  • #####################################################
  • hito-CPS1991-05//data/cry-00-00_data_000001.h5
  • hito-CPS1991-05//data/cry-00-00_data_000002.h5
  • hito-CPS1991-05//data/cry-00-00_data_000003.h5
  • hito-CPS1991-05//data/cry-00-00_data_000004.h5
  • hito-CPS1991-05//data/cry-00-00_master.h5
  • hito-CPS1991-05//data/cry-00-01_data_000001.h5
  • hito-CPS1991-05//data/cry-00-01_data_000002.h5
  • hito-CPS1991-05//data/cry-00-01_data_000003.h5
  • hito-CPS1991-05//data/cry-00-01_data_000004.h5
  • hito-CPS1991-05//data/cry-00-01_master.h5
  • hito-CPS1991-05//data/cry-00-02_data_000001.h5
  • hito-CPS1991-05//data/cry-00-02_data_000002.h5
  • hito-CPS1991-05//data/cry-00-02_data_000003.h5
  • hito-CPS1991-05//data/cry-00-02_data_000004.h5
  • hito-CPS1991-05//data/cry-00-02_master.h5
  • hito-CPS1991-05//data/cry-00-03_data_000001.h5
  • hito-CPS1991-05//data/cry-00-03_data_000002.h5
  • hito-CPS1991-05//data/cry-00-03_data_000003.h5
  • hito-CPS1991-05//data/cry-00-03_data_000004.h5
  • hito-CPS1991-05//data/cry-00-03_master.h5
  • hito-CPS1991-05//data/cry-00-04_data_000001.h5
  • hito-CPS1991-05//data/cry-00-04_data_000002.h5
  • hito-CPS1991-05//data/cry-00-04_data_000003.h5
  • hito-CPS1991-05//data/cry-00-04_data_000004.h5
  • hito-CPS1991-05//data/cry-00-04_master.h5
  • hito-CPS1991-05//data/cry-00-05_data_000001.h5
  • hito-CPS1991-05//data/cry-00-05_data_000002.h5
  • hito-CPS1991-05//data/cry-00-05_data_000003.h5
  • hito-CPS1991-05//data/cry-00-05_data_000004.h5
  • hito-CPS1991-05//data/cry-00-05_master.h5
  • hito-CPS1991-05//data/cry-00-06_data_000001.h5
  • hito-CPS1991-05//data/cry-00-06_data_000002.h5
  • hito-CPS1991-05//data/cry-00-06_data_000003.h5
  • hito-CPS1991-05//data/cry-00-06_data_000004.h5
  • hito-CPS1991-05//data/cry-00-06_master.h5

 

  • #####################################################
  • # 'cry-01-??_master.h5' are for 'full rotation helical' datasets
  • #####################################################
  • # 60.0 deg. / datasets
  • #  0.1 deg. / frame
  • #  600 frames / datasets
  • #  100 frames in each data file
  • # ** The program could collect 'full rotation' helical dataset
  • # but 60 deg. was set for this demonstration.
  • #####################################################
  • hito-CPS1991-05//data/cry-01-00_data_000001.h5
  • hito-CPS1991-05//data/cry-01-00_data_000002.h5
  • hito-CPS1991-05//data/cry-01-00_data_000003.h5
  • hito-CPS1991-05//data/cry-01-00_data_000004.h5
  • hito-CPS1991-05//data/cry-01-00_data_000005.h5
  • hito-CPS1991-05//data/cry-01-00_data_000006.h5
  • hito-CPS1991-05//data/cry-01-00_master.h5

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