Published March 7, 2018
| Version v1
Dataset
Open
USP5 Zf-UBD Differential Scanning Fluorimetry Assay Optimization
- 1. University of Toronto, Structural Genomics Consortium
Description
Optimization of a differential scanning fluorimetry (DSF) assay for USP5 zinc finger ubiquitin binding domain (Zf-UBD) to determine if the presence of detergent in buffer conditions results in significant difference in melting temperatures of USP5 Zf-UBD and if the addition of increasing concentrations of ubiquitin RLRGG peptide results in thermal shift of USP5 Zf-UBD.
Notes
Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.