10.5281/zenodo.1186158
https://zenodo.org/records/1186158
oai:zenodo.org:1186158
Wüest, Simon (Autor/in)
Simon (Autor/in)
Wüest
Hochschule Luzern
Rösch, Christian (Autor/in)
Christian (Autor/in)
Rösch
Hochschule Luzern
Ille, Fabian (Autor/in)
Fabian (Autor/in)
Ille
Hochschule Luzern
Egli, Marcel (Autor/in)
Marcel (Autor/in)
Egli
Hochschule Luzern
Calcium dependent current recordings in Xenopus laevis oocytes in microgravity
Zenodo
2017
Electrophysiology
Xenopus laevis oocyte
Calcium-dependent current
Microgravity
Parabolic flight
2017-01-01
Other
10.1016/j.actaastro.2017.10.003
10.5281/zenodo.1186157
https://zenodo.org/communities/lory
https://zenodo.org/communities/lory_hslu_t_und_a
https://zenodo.org/communities/lory_hslu
Creative Commons Attribution 4.0 International
Mechanical unloading by microgravity (or weightlessness) conditions triggers profound adaptation processes at the cellular and organ levels. Among other mechanisms, mechanosensitive ion channels are thought to play a key role in allowing cells to transduce mechanical forces. Previous experiments performed under microgravity have shown that gravity affects the gating properties of ion channels. Here, a method is described to record a calcium-dependent current in native Xenopus laevis oocytes under microgravity conditions during a parabolic flight. A 3-voltage-step protocol was applied to provoke a calcium-dependent current. This current increased with extracellular calcium concentration and could be reduced by applying extracellular gadolinium. The custom-made “OoClamp” hardware was validated by comparing the results of the 3-voltage-step protocol to results obtained with a well-established two-electrode voltage clamp (TEVC). In the context of the 2nd Swiss Parabolic Flight Campaign, we tested the OoClamp and the method. The setup and experiment protocol worked well in parabolic flight. A tendency that the calcium-dependent current was smaller under microgravity than under 1 g condition could be observed. However, a conclusive statement was not possible due to the small size of the data base that could be gathered
+ ID der Publikation: hslu_49893 + Art des Beitrages: Wissenschaftliche Medien + Jahrgang: 141 + Sprache: Englisch + Letzte Aktualisierung: 2018-02-28 12:59:09