Journal article Open Access

Metabolomics facilitates the discrimination of the specific anti-cancer effects of free- and polymer-conjugated doxorubicin in breast cancer models

Armiñán, Ana; Palomino-Schätzlein, Martina; Deladriere, Coralie; Arroyo-Crespo, Juan J.; Vicente-Ruiz,Sonia; Vicent, María Jesús; Pineda-Lucena, Antonio


JSON-LD (schema.org) Export

{
  "description": "<p>Metabolomics is becoming a relevant tool for understanding the molecular mechanisms<br>\ninvolved in the response to new drug delivery systems. The applicability of this experimental<br>\napproach to cell cultures and animal models makes metabolomics a useful tool for establishing<br>\ndirect connections between in vitro and in vivo data, thus providing a reliable platform for the<br>\ncharacterization of chemotherapeutic agents. Herein, we used metabolomic profiles based on<br>\nnuclear magnetic resonance (NMR) spectroscopy to evaluate the biochemical pathways involved in<br>\nthe response to a chemotherapeutic anthracycline drug (Doxorubicin, Dox) and an N-(2-<br>\nhydroxypropyl) methacrylamide (HPMA) copolymer-conjugated form (HPMA-Dox) in an in vitro<br>\ncell culture model and an in vivo orthotopic breast cancer model. We also used protein expression<br>\nand flow cytometry studies to obtain a better coverage of the biochemical alterations associated<br>\nwith the administration of these compounds. The overall analysis revealed that polymer conjugation<br>\nleads to increased apoptosis, reduced glycolysis, and reduced levels of phospholipids when<br>\ncompared to the free chemotherapeutic drug. Our results represent a first step in the application of<br>\nintegrated in vitro and in vivo metabolomic studies to the evaluation of drug delivery systems.</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Polymer Therapeutics Laboratory, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, Valencia, Spain.", 
      "@type": "Person", 
      "name": "Armi\u00f1\u00e1n, Ana"
    }, 
    {
      "affiliation": "Joint Research Unit in Clinical Metabolomics, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe / Instituto de Investigaci\u00f3n Sanitaria La Fe, Valencia, Spain.", 
      "@type": "Person", 
      "name": "Palomino-Sch\u00e4tzlein, Martina"
    }, 
    {
      "affiliation": "Polymer Therapeutics Laboratory, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, Valencia, Spain", 
      "@type": "Person", 
      "name": "Deladriere, Coralie"
    }, 
    {
      "affiliation": "Polymer Therapeutics Laboratory, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, Valencia, Spain", 
      "@type": "Person", 
      "name": "Arroyo-Crespo, Juan J."
    }, 
    {
      "affiliation": "Polymer Therapeutics Laboratory, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, Valencia, Spain", 
      "@type": "Person", 
      "name": "Vicente-Ruiz,Sonia"
    }, 
    {
      "affiliation": "Polymer Therapeutics Laboratory, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, Valencia, Spain", 
      "@type": "Person", 
      "name": "Vicent, Mar\u00eda Jes\u00fas"
    }, 
    {
      "affiliation": "de Investigaci\u00f3n Sanitaria La Fe, Valencia, Spain. / Drug Discovery Unit, Instituto de Investigaci\u00f3n Sanitaria La Fe, Valencia, Spain.", 
      "@type": "Person", 
      "name": "Pineda-Lucena, Antonio"
    }
  ], 
  "headline": "Metabolomics facilitates the discrimination of the specific anti-cancer effects of free- and polymer-conjugated doxorubicin in breast cancer models", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2018-02-08", 
  "url": "https://zenodo.org/record/1175906", 
  "keywords": [
    "metabolomics", 
    "NMR", 
    "nanomedicine", 
    "polymer therapeutics", 
    "breast cancer"
  ], 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.1016/j.biomaterials.2018.02.015", 
  "@id": "https://doi.org/10.1016/j.biomaterials.2018.02.015", 
  "@type": "ScholarlyArticle", 
  "name": "Metabolomics facilitates the discrimination of the specific anti-cancer effects of free- and polymer-conjugated doxorubicin in breast cancer models"
}
61
82
views
downloads
Views 61
Downloads 82
Data volume 366.9 MB
Unique views 61
Unique downloads 81

Share

Cite as