10.23868/201707029
https://zenodo.org/records/1175806
oai:zenodo.org:1175806
Tenchurin, T.H.
T.H.
Tenchurin
0000-0003-1000-6003
National Research Center «Kurchatov Institute»
Lyundup, A.V.
A.V.
Lyundup
0000-0002-0102-5491
Sechenov First Moscow State Medical University
Demchenko, A.G.
A.G.
Demchenko
Sechenov First Moscow State Medical University
Krasheninnikov, M.E.
M.E.
Krasheninnikov
0000-0002-3574-4013
Sechenov First Moscow State Medical University
Balyasin, M.V.
M.V.
Balyasin
0000-0002-3097-344X
Sechenov First Moscow State Medical University
Klabukov, I.D.
I.D.
Klabukov
0000-0002-2888-7999
Sechenov First Moscow State Medical University
Shepelev, A.D.
A.D.
Shepelev
National Research Center «Kurchatov Institute»
Mamagulashvili, V.G.
V.G.
Mamagulashvili
National Research Center «Kurchatov Institute»
Orehov, A.S.
A.S.
Orehov
National Research Center «Kurchatov Institute»
Chvalun, S.N.
S.N.
Chvalun
0000-0001-9405-4509
National Research Center «Kurchatov Institute»
Dyuzheva, T.G.
T.G.
Dyuzheva
0000-0003-0573-7573
Sechenov First Moscow State Medical University
Modification of biodegradable fibrous scaffolds with Epidermal Growth Factor by emulsion electrospinning for promotion of epithelial cells proliferation
Zenodo
2017
electrospinning
Epidermal Growth Factor
cell proliferation
tissue engineering
cell proliferation
biodegradable plastics
polycaprolactone
extracellular matrix
electrospinning
EGF
GFP
epithelial cells
2017-12-15
rus
Creative Commons Attribution 4.0 International
Supporting of a physiologically relevant cellular microenvironment is currently a grand challenge in the design of tissue-engineering grafts based on biocompatible and biodegradable polymeric materials. The aim of this research was to develop a new technique of a fibrous polycaprolactone-based scaffold modification with epidermal growth factor (EGF) and assessment of its effect on scaffold properties and proliferative activity of epithelial cells in vitro. Fibrous scaffolds from EGF-functionalized polycaprolactone has received by the emulsion electrospinning method. Prolonged yield of EGF upon the material destruction and its biological effect on the MCF7 cell line proliferation have been estimated using ELISA and iCELLigence real-time cell analysis for respectively.