Journal article Closed Access
Grounda, Athina; Nychas, George John; Panagou, Efstathios
The aim of this study was to evaluate the survival of Salmonella, Escherichia coli O157:H7, Listeria monocytogenes, and Staphylococcus aureus on natural black olives during aerobic storage without brine and conclude about the safety of this fermented food. Fermented black olives (pH 3.95; NaCl 6.02%) were artificially inoculated with 4 strains of S. enterica ser. Enteritidis, 4 strains of S. enterica ser. Typhimurium, 3 strains of E. coli O157:H7, 3 strains of L. monocytogenes, and 3 strains of S. aureus either in monoculture or in mixed culture by submerging the fruits in a solution containing ca. 7.0 log CFU/ml of each target pathogen. Olives were subsequently stored aerobically at 4 and 20°C, and pathogens were recovered on selective media for a time period of 15 days. Enrichment was employed to define the presence/absence of the target pathogens when their counts were below the detection limit of direct plating. Results showed that the population of all pathogens presented a rapid decline within the first 2 days of storage depending on storage temperature, type of inoculation (monoculture or cocktail) and strain. Storage at 4°C seemed to prolong the survival of certain strains of pathogens compared to 20°C by ca. 1 day. Enrichment revealed absence (< 1 cfu/25g) of Salmonella and E. coli O157:H7 from olive samples with the exception of all strains of L. monocytogenes at 4°C and one strain at 20°C that were detected by selective enrichment until the end of storage. The results of this study indicate that natural black olives are not a favourable environment to support the growth of the investigated pathogens after post-processing contamination provided that olives have been subjected to proper fermentation process.
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