Journal article Open Access

Solvent Effect on Antioxidant Activity and Total Phenolic Content of Betula alba and Convolvulus arvensi

Mohd Azman A. Nurul; Husni Shafik; Almajano P. Maria; Gallego G. Maria

The potential of using herbal Betula alba (BA) and Convolvulus arvensis (CA) as a natural antioxidant for food applications were investigated. Each plant extract was prepared by using pure ethanol, different concentration of ethanol aqueous solutions, including 50% and 75%, 50% methanol aqueous and water. Total phenolic content (TPC) was determined using Folin–Ciocalteau method and antioxidant activity were analyzed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, trolox equivalent antioxidant capacity (TEAC), Oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) respectively. Ethanol extract of CA exhibited the highest TPC and antioxidant activity; however BA showed varies of antioxidant activity value in each assay. The BA and CA exhibit the potential sources of natural antioxidant for food commodities.

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  • D. A. Einzhammer, and Z. Q. Xu, "Betulinic acid: a promising anticancer candidate," IDrugs, vol. 4, Apr. 2004, pp. 359–373.

  • D. Thurnher, D. Turhani, M. Pelzmann, B. Wannemacher, B. Knerer, M. Formanek, V. Wacheck, and E. Selzer, " Betulinic acid: a new cytotoxic compound against malignant head and neck cancer cells," Head Neck., vol. 25, Sep. 2003, pp. 732–740. [10] C. Eder-Czembirek, C. Czembirek, B. M. Erovic, E. Selzer, D. Turhani, L. Vormittag, and D. Thurnher, "Combination of betulinic acid with cisplatin-different cytotoxic effects in two head and neck cancer cell lines," Oncol Rep., vol. 14, Sep. 2005, pp.667–671. [11] H. Ehrhardt, S. Fulda, M. Fuhrer, K. M. Debatin, and I. Jeremias, "Betulinic acid-induced apoptosis in leukemia cells," Leukemia., vol. 8, Aug. 2004, pp. 1406–1412. [12] D. V. Raghuvar Gopal, A. A. Nakar, Y. Badrinath, K. P. Mishra, and D. S. Joshi, "Betulinic acid induces apoptosis in human chronic myelogenous leukemia (CML) cell line K-562 without altering the levels of Bcr-Abl," Toxicol Lett., vol. 15, Mar. 2005, pp.343–351. [13] C. Roberto, Medicinal Plants. London: The Macdonald & Co., 1982, ch. 95. [14] Al-Bowait, I. F. Albokhadaim, and A. M. Homieda, "Immunostimulant Effect of Bindweed (Convolvulus Arvensis) Extract in Rabbit," Research Journal of Pharmacology, vol. 4, 2010, pp. 51-54. [15] H. Sadeghi-aliabadi, N. Ghasemi, and M. Kohi, "Cytotoxic effect of Convolvulus arvensis extracts on human cancerous cell line," Research in Pharmaceutical Sciences, vol. 3, Apr. 2008, pp. 31-34. [16] J. Santas, R. Carbo, M. H. Gordon, and M. P. Almajano, "Comparison of the antioxidant activity of two Spanish onion varieties," Food Chem., vol. 107, Apr. 2008, pp. 1210–1216. [17] S. Zhang, H. Bi, and C. Liu, "Extraction of bio-active components from Rhodiola sachalinensis under ultrahigh hydrostatic pressure," Sep. Purif. Technol., vol. 57, Oct. 2007, pp. 275−280. [18] L. Yu, S. Haley, J. Perret, and M. Harris, "Antioxidant properties of hard winter wheat extracts," Food Chem., vol. 78, Sep. 2002, pp. 457–461. [19] A. Moure, J. M. Cruz, D. Franco, H. Dominguez, J. Sineiro, H. Dominguez, M. J. Núnez, and J. C. Parajó, "Natural antioxidants from residual sources," Food Chem., vol. 72, Feb. 2001, pp.145–171. [20] M. Al-Farsi, and C. Y. Lee, "Optimization of phenolic and dietary fibre extraction from date seeds," Food Chem., vol. 108, Jun. 2008, pp. 977–985. [21] A. Fernández-Agullóa, E. Pereira, M.S. Freire, P. Valentãoc, P.B. Andradec, J.González-Álvareza, and J.A. Pereira, " Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts," Ind Crops Prod., vol. 42, Mar. 2013, pp. 126– 132. [22] R. Prior, X. Wu, and K. Schaich, "Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements," J. Agric. Food. Chem., vol. 53, May 2005, pp. 4290–4302. [23] G. Spigno, L. Tramelli, and D.M. De Faveri, "Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics," J. Food Eng., vol. 81, Jul. 2007, pp. 200–208. [24] K. K. Chew, M. Z. Khoo, S. Y. Ng, Y. Y. Thoo, W. M. Wan Aida, and C. W. Ho, "Effect of ethanol concentration, extraction time and extraction temperature on the recovery of phenolic compounds and antioxidant capacity of Orthosiphon stamineus extracts," IFRJ, vol. 18, 2011, pp. 1427–1435. [25] L. M. Magalhaes, M. A. Segundo, S. Reis, and J. L. F. C. Lima, "Methodological aspects about in vitro evaluation of antioxidant properties," Analytica Chimica ACTA, vol. 613, 2008, pp.1-19. [26] A. Khoddami, A. W. Meredith, and H. R. Thomas, "Techniques for Analysis of Plant Phenolic Compounds," Molecules, vol. 18, 2013, pp. 2328-2375. [27] R. Jussi-Pekka, S. Remesa, M. Heinonenb, A. Hopiab, M. Kahkonenb, T.Kujalac, K. Pihlajac, H. Vuorelaa, and P. Vuorelaa, "Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds," Int J Food Microbiol., vol. 56, May 2000, pp.3–12. [28] W. Rzeski, A. Stepulak, M. Szymański, M. Sifringer, J. Kaczor, K. Wejksza, B. Zdzisińska, and M. Kandefer-Szerszeń, "Betulinic acid decreases expression of bcl-2 and cyclin D1, inhibits proliferation, migration and induces apoptosis in cancer cells," Naunyn-Schmiedeberg's Arch Pharmacol., vol. 374, Oct. 2006, pp. 11–20. [29] U. Jermnak, T.Yoshinari, Y.Sugiyama, R. Tsuyuki, H. Nagasawa, and S. Sakuda, "Isolation of methyl syringate as a specific aflatoxin production inhibitor from the essential oil of Betula alba and aflatoxin production inhibitory activities of its related compounds," Int J Food Microbiol., vol. 153, Feb. 2012, pp. 339–344. [30] H. A. Attia, M. Samar, and M. Mouneir, "Antidiarrhoeal activity of some Egyptian medicinal plant extracts," J. Ethnopharmacol., vol. 92, Jun. 2004, pp. 303–309.

  • I. F.F. Benzie, and J. J. Strain, "The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay," Anal. Biochem., vol.239, Jul. 1996, pp. 70-76.

  • J. I. García-Plazaola, A. Hernández, and J. M. Becerril, "Antioxidant and Pigment Composition during Autumnal Leaf Senescence in Woody Deciduous Species Differing in their Ecological Traits," Plant biol., vol. 5, Sept. 2003, pp. 557-566.

  • K. Stockham, R. Paimin, J. D. Orbell, P. Adorno, and S. Buddhadasa, "Modes of handling Oxygen Radical Absorbance Capacity (ORAC) data and reporting values in product labeling," J. Food Compos. Anal., vol. 24, Jun. 2011, pp.686-691.

  • N. J. Miller, J. Sampson, L. P. Candeias, P. M. Bramley, and C. A. RiceEvans, "Antioxidant activities of carotenes and xanthophylls," FEBS Lett., vol.384, Apr. 1998, pp.240-242.

  • O. 1. Aruoma, "Antioxidant actions of plant foods, use of oxidative DNA damage as a tool for studying antioxidant efficacy," Free Radical Res., vol. 30, Jun. 1999, pp. 419-427.

  • O. I. Aruoma, "Free radicals, oxidative stress and antioxidants in human health and disease," J. Am. Oil Chem. Soc., vol. 75, 1998, pp.199-212.

  • T. Madhujith, and F. Shahidi, "Optimization of the extraction of antioxidative constituents of six barley cultivars and their antioxidant properties," J. Agric. Food. Chem., vol. 54, Oct. 2006, pp. 8048-8057.

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