Journal article Open Access

Biorecognizable Nanoparticles Based On Hyaluronic Acid/Poly(ε-Caprolactone) Block Copolymer

Jong Ho Hwang; Dae Hwan Kang; Young-IL Jeong

Since hyaluronic acid (HA) receptor such as CD44 is
over-expressed at sites of cancer cells, HA can be used as a targeting
vehicles for anti-cancer drugs. The aim of this study is to synthesize
block copolymer composed of hyaluronic acid and
poly(ε-caprolactone) (HAPCL) and to fabricate polymeric micelles for
anticancer drug targeting against CD44 receptor of tumor cells.
Chemical composition of HAPCL was confirmed using 1H NMR
spectroscopy. Doxorubicin (DOX) was incorporated into polymeric
micelles of HAPCL. The diameters of HAPHS polymeric micelles
were changed around 80nm and have spherical shapes. Targeting
potential was investigated using CD44-overexpressing. When
DOX-incorporated polymeric micelles was added to KB cells, they
revealed strong red fluorescence color while blocking of CD44
receptor by pretreatment of free HA resulted in reduced intensity,
indicating that HAPCL polymeric micelles have targetability against
CD44 receptor.

Files (761.0 kB)
Name Size
16466.pdf
md5:7c6ba94efcc5905f34484998131f06bd
761.0 kB Download
  • <p>

  • X. Huang, Y.I. Jeong, B.K. Moon, L. Zhang, D.H. Kang, I. Kim, "Self-assembly of morphology-tunable architectures from tetraarylmethane derivatives for targeted drug delivery," Langmuir, vol. 29, no. 10, pp. 3223-3333, 2013.

  • S. Xu, Q. Xu, J. Zhou, J. Wang, N. Zhang, L. Zhang, "Preparation and characterization of folate-chitosan-gemcitabine core-shell nanoparticles for potential tumor-targeted drug delivery," J Nanosci. Nanotechnol. vol. 13, no. 1, pp. 129-138, 2013.

  • Y. Bae, W.D. Jang, N. Nishiyama, S. Fukushima, K. Kataoka. "Multifunctional polymeric micelles with folate-mediated cancer cell targeting and pH-triggered drug releasing properties for active intracellular drug delivery," Mol. Biosyst. vol. 1, no. 3, pp. 242-250, 2005.

  • V. Ladeda, J.A. Aguirre Ghiso, E. Bal de Kier Joffe, "Function and expression of CD44 during spreading, migration, and invasion of murine carcinoma cells," Exp. Cell Res., vol. 242, pp. 515-527, 1998.

  • Y. Akiyama, S. Jung, B. Salhia, S. Lee, S. Hubbard, M. Taylor, T. Mainprize, K. Akaishi, W. Furth, J. T. Rutka, "Hyaluronate receptors mediating glioma cell migration and proliferation," J. Neuro-Oncol., vol. 53, pp. 115–127, 2001.

  • E. Auzenne, S.C. Ghosh, M. Khodadadian, B. Rivera, D. Farquhar, R.E. Price, M. Ravoori, V. Kundra, R.S. Freedman, J. Klostergaard, "Hyaluronic acid-paclitaxel: antitumor efficacy against CD44(+) human ovarian carcinoma xenografts," Neoplasia, vol. 9, pp. 479-486, 2007.

  • N. Senzer, J. Nemunaitis, D. Nemunaitis, C. Bedell, G. Edelman, M. Barve, R. Nunan, K. F. Pirollo, A. Rait, E.H. Chang, "Phase I study of a systemically delivered p53 nanoparticle in advanced solid tumors," Mol Ther. vol. 21, no. 5, pp. 1096-1103, 2013.

  • Y.I. Jeong, D.H. Kim, C.W. Chung, J.J. Yoo, K.H. Choi, C.H. Kim, S.H. Ha, D.H. Kang, "Self-assembled nanoparticles of hyaluronic acid/poly(DL-lactide-co-glycolide) block copolymer," Colloids Surf B Biointerfaces. vol. 90, pp. 28-35, 2012.

  • Y. Luo, G.D. Prestwich, "Synthesis and selective cytotoxicity of a hyaluronic acid–antitumor bioconjugate," Bioconj. Chem., vol. 10, pp. 755–763, 1999. [10] Y. Luo, N.J. Bernshaw, Z.R. Lu, J. Kopecek, G.D. Prestwich, "Targeted delivery of doxorubicin by HPMA copolymer-hyaluronan bioconjugates," Pharm. Res., vol. 19, pp. 396-402, 2002. [11] E. Segawa, H. Kishimoto, K. Takaoka, K. Noguchi, S. Hashitani, K. Sakurai, M. Urade, "Promotion of hematogenous metastatic potentials in human KB carcinoma cells with overexpression of cyclooxygenase-2," Oncol. Rep. vol. 24, no. 3, pp. 733-739, 2010.</p>

20
9
views
downloads
All versions This version
Views 2020
Downloads 99
Data volume 6.8 MB6.8 MB
Unique views 2020
Unique downloads 99

Share

Cite as