Other Open Access
Kumar, Abhishek; Ouyang, Mingxing; Van den Dries, Koen; McGhee, Ewan James; Tanaka, Keiichiro; Anderson, Marie D.; Groisman, Alexander; Goult, Benjamin T.; Anderson, Kurt I.; Schwartz, Martin A.
<?xml version='1.0' encoding='utf-8'?> <oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"> <dc:creator>Kumar, Abhishek</dc:creator> <dc:creator>Ouyang, Mingxing</dc:creator> <dc:creator>Van den Dries, Koen</dc:creator> <dc:creator>McGhee, Ewan James</dc:creator> <dc:creator>Tanaka, Keiichiro</dc:creator> <dc:creator>Anderson, Marie D.</dc:creator> <dc:creator>Groisman, Alexander</dc:creator> <dc:creator>Goult, Benjamin T.</dc:creator> <dc:creator>Anderson, Kurt I.</dc:creator> <dc:creator>Schwartz, Martin A.</dc:creator> <dc:date>2016-05-09</dc:date> <dc:description>Integrin-dependent adhesions are mechanosensitive structures in which talin mediates a linkage to actin filaments either directly or indirectly by recruiting vinculin. Here, we report the development and validation of a talin tension sensor. We find that talin in focal adhesions is under tension, which is higher in peripheral than central adhesions. Tension on talin is increased by vinculin and depends mainly on actin-binding site 2 (ABS2) within the middle of the rod domain, rather than ABS3 at the far C terminus. Unlike vinculin, talin is under lower tension on soft substrates. The difference between central and peripheral adhesions requires ABS3 but not vinculin or ABS2. However, differential stiffness sensing by talin requires ABS2 but not vinculin or ABS3. These results indicate that central versus peripheral adhesions must be organized and regulated differently, and that ABS2 and ABS3 have distinct functions in spatial variations and stiffness sensing. Overall, these results shed new light on talin function and constrain models for cellular mechanosensing.</dc:description> <dc:identifier>https://zenodo.org/record/1066336</dc:identifier> <dc:identifier>10.1083/jcb.201510012</dc:identifier> <dc:identifier>oai:zenodo.org:1066336</dc:identifier> <dc:rights>info:eu-repo/semantics/openAccess</dc:rights> <dc:title>Talin tension sensor reveals novel features of focal adhesion force transmission and mechanosensitivity</dc:title> <dc:type>info:eu-repo/semantics/other</dc:type> <dc:type>publication-other</dc:type> </oai_dc:dc>