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Manzoor Atif; Abdin Ul Zain; Shaina Hoor; Webb A. Bruce; Jamil Amer

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  <identifier identifierType="DOI">10.5281/zenodo.1064667</identifier>
      <creatorName>Manzoor Atif</creatorName>
      <affiliation>Department of Entomology, University of Agriculture Faisalabad, Pakistan</affiliation>
      <creatorName>Abdin Ul Zain</creatorName>
      <affiliation>Department of Entomology, University of Agriculture Faisalabad, Pakistan</affiliation>
      <creatorName>Shaina Hoor</creatorName>
      <affiliation>Department of Entomology, University of Agriculture Faisalabad, Pakistan</affiliation>
      <creatorName>Webb A. Bruce</creatorName>
      <affiliation>Department of Entomology, University of Kentucky, Lexington, USA</affiliation>
      <creatorName>Jamil Amer</creatorName>
      <affiliation>Department of Biochemistry, University of Agriculture Faisalabad, Pakistan</affiliation>
    <subject>Bracon hebetor, cell line, venom, VRE, parasitoids</subject>
    <date dateType="Issued">2017-11-22</date>
  <resourceType resourceTypeGeneral="JournalArticle"/>
    <alternateIdentifier alternateIdentifierType="url"></alternateIdentifier>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.1064666</relatedIdentifier>
    <rights rightsURI="">Creative Commons Attribution Share Alike 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
    <description descriptionType="Abstract">&lt;p&gt;Insect cell cultures are widely used in studies of insect cell physiology, toxicology, developmental biology and microbial pathology. The lethal effects of crude venom extracted from the ectoparasitic wasp &lt;em&gt;Bracon hebetor &lt;/em&gt;were examined with three cultured insect cell lines; &lt;em&gt;Spodoptera frugiperda &lt;/em&gt;(Sf9), &lt;em&gt;Tribolium castaneum &lt;/em&gt;(TcA) and &lt;em&gt;Aedes aegypti &lt;/em&gt;(Aag-2). Venom caused cells to round-up, swell and eventually die. Despite similar sensitivities and overlapping LC&lt;sub&gt;50&lt;/sub&gt; values [(0.00125-0.00695) venom reservoir equivalents (VRE)/&amp;micro;l], significant differences were noted at the onset of cytotoxicity among the three insect cell lines. Cells from the &lt;em&gt;Tribolium castaneum &lt;/em&gt;(TcA) and &lt;em&gt;Aedes aegypti &lt;/em&gt;(Aag-2) showed little sensitivity to the venom: 0.0046 VRE were needed to induce 50% mortality in TcA [50% lethal concentration (LC&lt;sub&gt;50&lt;/sub&gt;) = 0.0046 VRE/&amp;micro;l], and 0.0069 VRE were needed to induce 50% mortality in Aag-2 [50% lethal concentration (LC&lt;sub&gt;50&lt;/sub&gt;) = 0.0069 VRE/&amp;micro;l). Over 80% of the Sf9 cells were nonviable within 1 h after the addition of an LC&lt;sub&gt;99&lt;/sub&gt; dose of venom, whereas the other cells required a 5-10-fold longer incubation period to produce mortality above 50%.&lt;/p&gt;</description>
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