Journal article Open Access

FUNCTIONAL ANALYSIS OF BRACON HEBETOR VENOM ON TARGET AND NON-TARGET INSECT CELL LINES

Manzoor Atif; Abdin Ul Zain; Shaina Hoor; Webb A. Bruce; Jamil Amer


DCAT Export

<?xml version='1.0' encoding='utf-8'?>
<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:adms="http://www.w3.org/ns/adms#" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dct="http://purl.org/dc/terms/" xmlns:dctype="http://purl.org/dc/dcmitype/" xmlns:dcat="http://www.w3.org/ns/dcat#" xmlns:duv="http://www.w3.org/ns/duv#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:frapo="http://purl.org/cerif/frapo/" xmlns:geo="http://www.w3.org/2003/01/geo/wgs84_pos#" xmlns:gsp="http://www.opengis.net/ont/geosparql#" xmlns:locn="http://www.w3.org/ns/locn#" xmlns:org="http://www.w3.org/ns/org#" xmlns:owl="http://www.w3.org/2002/07/owl#" xmlns:prov="http://www.w3.org/ns/prov#" xmlns:rdfs="http://www.w3.org/2000/01/rdf-schema#" xmlns:schema="http://schema.org/" xmlns:skos="http://www.w3.org/2004/02/skos/core#" xmlns:vcard="http://www.w3.org/2006/vcard/ns#" xmlns:wdrs="http://www.w3.org/2007/05/powder-s#">
  <rdf:Description rdf:about="https://doi.org/10.5281/zenodo.1064667">
    <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#anyURI">https://doi.org/10.5281/zenodo.1064667</dct:identifier>
    <foaf:page rdf:resource="https://doi.org/10.5281/zenodo.1064667"/>
    <dct:creator>
      <rdf:Description>
        <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/>
        <foaf:name>Manzoor Atif</foaf:name>
        <org:memberOf>
          <foaf:Organization>
            <foaf:name>Department of Entomology, University of Agriculture Faisalabad, Pakistan</foaf:name>
          </foaf:Organization>
        </org:memberOf>
      </rdf:Description>
    </dct:creator>
    <dct:creator>
      <rdf:Description>
        <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/>
        <foaf:name>Abdin Ul Zain</foaf:name>
        <org:memberOf>
          <foaf:Organization>
            <foaf:name>Department of Entomology, University of Agriculture Faisalabad, Pakistan</foaf:name>
          </foaf:Organization>
        </org:memberOf>
      </rdf:Description>
    </dct:creator>
    <dct:creator>
      <rdf:Description>
        <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/>
        <foaf:name>Shaina Hoor</foaf:name>
        <org:memberOf>
          <foaf:Organization>
            <foaf:name>Department of Entomology, University of Agriculture Faisalabad, Pakistan</foaf:name>
          </foaf:Organization>
        </org:memberOf>
      </rdf:Description>
    </dct:creator>
    <dct:creator>
      <rdf:Description>
        <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/>
        <foaf:name>Webb A. Bruce</foaf:name>
        <org:memberOf>
          <foaf:Organization>
            <foaf:name>Department of Entomology, University of Kentucky, Lexington, USA</foaf:name>
          </foaf:Organization>
        </org:memberOf>
      </rdf:Description>
    </dct:creator>
    <dct:creator>
      <rdf:Description>
        <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/>
        <foaf:name>Jamil Amer</foaf:name>
        <org:memberOf>
          <foaf:Organization>
            <foaf:name>Department of Biochemistry, University of Agriculture Faisalabad, Pakistan</foaf:name>
          </foaf:Organization>
        </org:memberOf>
      </rdf:Description>
    </dct:creator>
    <dct:title>FUNCTIONAL ANALYSIS OF BRACON HEBETOR VENOM ON TARGET AND NON-TARGET INSECT CELL LINES</dct:title>
    <dct:publisher>
      <foaf:Agent>
        <foaf:name>Zenodo</foaf:name>
      </foaf:Agent>
    </dct:publisher>
    <dct:issued rdf:datatype="http://www.w3.org/2001/XMLSchema#gYear">2017</dct:issued>
    <dcat:keyword>Bracon hebetor, cell line, venom, VRE, parasitoids</dcat:keyword>
    <dct:issued rdf:datatype="http://www.w3.org/2001/XMLSchema#date">2017-11-22</dct:issued>
    <dct:language rdf:resource="http://publications.europa.eu/resource/authority/language/ENG"/>
    <owl:sameAs rdf:resource="https://zenodo.org/record/1064667"/>
    <adms:identifier>
      <adms:Identifier>
        <skos:notation rdf:datatype="http://www.w3.org/2001/XMLSchema#anyURI">https://zenodo.org/record/1064667</skos:notation>
        <adms:schemeAgency>url</adms:schemeAgency>
      </adms:Identifier>
    </adms:identifier>
    <dct:isVersionOf rdf:resource="https://doi.org/10.5281/zenodo.1064666"/>
    <dct:description>&lt;p&gt;Insect cell cultures are widely used in studies of insect cell physiology, toxicology, developmental biology and microbial pathology. The lethal effects of crude venom extracted from the ectoparasitic wasp &lt;em&gt;Bracon hebetor &lt;/em&gt;were examined with three cultured insect cell lines; &lt;em&gt;Spodoptera frugiperda &lt;/em&gt;(Sf9), &lt;em&gt;Tribolium castaneum &lt;/em&gt;(TcA) and &lt;em&gt;Aedes aegypti &lt;/em&gt;(Aag-2). Venom caused cells to round-up, swell and eventually die. Despite similar sensitivities and overlapping LC&lt;sub&gt;50&lt;/sub&gt; values [(0.00125-0.00695) venom reservoir equivalents (VRE)/&amp;micro;l], significant differences were noted at the onset of cytotoxicity among the three insect cell lines. Cells from the &lt;em&gt;Tribolium castaneum &lt;/em&gt;(TcA) and &lt;em&gt;Aedes aegypti &lt;/em&gt;(Aag-2) showed little sensitivity to the venom: 0.0046 VRE were needed to induce 50% mortality in TcA [50% lethal concentration (LC&lt;sub&gt;50&lt;/sub&gt;) = 0.0046 VRE/&amp;micro;l], and 0.0069 VRE were needed to induce 50% mortality in Aag-2 [50% lethal concentration (LC&lt;sub&gt;50&lt;/sub&gt;) = 0.0069 VRE/&amp;micro;l). Over 80% of the Sf9 cells were nonviable within 1 h after the addition of an LC&lt;sub&gt;99&lt;/sub&gt; dose of venom, whereas the other cells required a 5-10-fold longer incubation period to produce mortality above 50%.&lt;/p&gt;</dct:description>
    <dct:description>{"references": ["Abt, M., &amp; Rivers, D. B. (2007). Characterization of phenoloxidase activity in venom from the ectoparasitoid Nasonia vitripennis (Walker) (Hymenoptera: Pteromalidae). Journal of Invertebrate Pathology, 94, 108-118. Beard, R. L. (1978). Venoms of Braconidae. In: Bettini, S. (Ed.), Handbuch der Experimentellen Pharmakologie (Manual for Experimental Pharmacology). Arthropod Venoms, 48, 773-800. Beard, R. L. (1952). The toxicology of Habrobracon venom: A study of a natural insecticide. Bulletin of the Connecticut Agricultural Experiment Station, 562, 1-27. Cline, L. D., Press, J. W., &amp; Flaherty, B. R. (1984). Preventing the spread of the Almond Moth (Lepidoptera: Pyralidae) from infested food debris to adjacent un-infested packages, using the Parasite Bracon hebetor (Hymenoptera: Braconidae). Journal of Economic Entomology, 77, 331-333. Coudron, T. A., &amp; Puttler, B. (1988). Response of natural and factitious hosts to the ectoparasite Euplectrus plathypenae (Hymenoptera: Eulophidae). Annual Entomological Society of. America, 81, 931-937. Coudron, T. A. (1991). Host-regulating factors associated with parasitic Hymenoptera. In: Hedin, P.A. (Ed.), Naturally Occurring Pest Regulators, ASC Symposium Series. American Chemical Society, Washington, 449, 41-65. Darwish, E., El-Shazly, M., &amp; El-Sherif, H. (2003). The choice of probing sites by Bracon hebetor (Say) (Hymenoptera: Braconidae) foraging for Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Journal of Stored Product Research, 39, 265-276. Doury, G., Bigot, Y., &amp; Periquet, G. (1997). Physiological and biochemical analysis of factors in the female venom gland and larval salivary secretions of the ectoparasitoid wasp Eupelmus orientalis. Journal of Insect Physiology, 43, 69-81. Drenth, D. (1973). Susceptibility of different species of insects to an extract of the venom gland of the wasp Microbracon hebetor (Say). Toxicon, 12, 189-192. Edwards, S., &amp; Sernka, T.J. (1969). On the action of Bracon venom. Toxicon, 6, 303-305. Feyereisen, R. (1995). Molecular biology of insecticide resistance. Toxicology Letters, 82/83, 83-90. Finney, D. J. (1971). Probit Analysis, 3rd Edn. Cambridge University Press, 333 pp. Gul, M., &amp; Gulel, A. (1995): Parasitoid Bracon hebetor (Say.) (Hymenoptera: Braconidae) \u00d5un biyolojisi ve konak larva buyuklu\u00dbunun verim ve e\u00dfey oran\u00f5 uzerine etkisi. Turkish Journal of Zoology, 19, 231-235. Gurevitz, M. (2010). A deadly scorpion provides a safe pesticide. Science news. http://www.sciencedaily.com. Heimpel, G.E., Antolin, M.F., Franqui, R.A., &amp; Strand, M.R. (1997). Reproductive isolation and genetic variation between two ''strains'' of Bracon hebetor (Hymenoptera: Braconidae). Biological Control, 9, 149-156. Khalil, M. S., Raza, A. B. M., Afzal, M., Aqueel, M. A., Khalil, H., &amp; Hance, T. (2016). Effects of different host species on the life history of Bracon hebetor. Animal Biology, 66(3-4), 403-414. Leng, P., Zhang, Z., Pan, G., &amp; Zhao, M. (2011). Applications and development trends in biopesticides. African Journal of Biotechnology, 10(86), 19864-19873. Piek, T., &amp; Engels, E. (1969). Action of the venom of Microbracon hebetor Say on larvae and adults of Phzlosamia cynthia Hiibn. Comparative Biochemistry and Physiology, 28, 603-618. Piek, T., &amp; Thomas, R.T. (1969). Paralysing venoms of solitary wasps. Comparative Biochemistry and Physiology, 30(1), 13-31. Piek, T. (1966). Site of action of venom of Microbracon hebetor (Say.) (Hymenoptera: Braconidae). Journal of Insect Physiology, 12, 561-568. Piek, T.W., Spanjer, W., Njio, K.D., Veenendaal, R.L., &amp; Mantel, P. (1974). Paralysis caused by the venom of the wasp Microbracon gelechiae. Journal of Insect Physiology, 20, 2307-2319. Piek, T., &amp; Spanjer, W. (1986). Chemistry and pharmacology of solitary wasp venoms. In: Venoms of Hymenoptera, Biochemical, Pharmacological and Behavioral Aspects, pp. 161-308. Piek, T. (1986). Venoms of the Hymenoptera. Biochemical, pharmacological and Behavioural Aspects.Academic Press; London, UK, 570pp. Pretty, J., &amp; Bharucha, Z. P. (2015). Integrated pest management for sustainable intensification of agriculture in Asia and Africa. Insects, 6(1), 152-182. Quistad, G. B., Dennis, P. A., Skinner, W. S. (1992). Insecticidal activity of spider (Araneae), centipede (Chilopoda), scorpion (Scorpionida), and snake (Serpentes) venoms. Journal of Economic Entomology, 85, 33-39. Rivers, D. B., Hink, W. F., &amp; Denlinger, D. L. (1993). Toxicity of the venom from Nasonia vitripennis (Hymenoptera: Pteromalidae) toward fly hosts, non-target insects, different developmental stages, and cultured insect cells. Toxicon, 31(6), 755-765. Rivers, D. B., Genco, M., &amp; Sanchez, R. A. (1999). In vitro analysis of venom from the wasp Nasonia vitripennis: susceptibility of different cell lines and venom-induced changes in plasma membrane permeability. In Vitro Cellular &amp; Developmental Biology-Animal, 35(2), 102-110. Stoddart, M. J. (2011). Mammalian cell viability: methods and protocols. Humana Press, 240pp. Tamashiro, M. (1971). A biological study of the venoms of two species of Bracon. Hawaii Agr. Exp. Sta. Tech. Bull., 70, 1-52. Yu, D. S., van Achterberg, C., &amp; Horstmann, K. (2012). Taxapad 2012\u2013World Ichneumonoidae 2011. Taxonomy, Biology, Morphology and Distribution. On USB Flash drive. Ottawa, Ontario, Canada. Zhang, Z., Ye, G.Y., Cai, J., &amp; Hu, C. (2005). Comparative venom toxicity between Pteromalus puparum and Nasonia vitripennis (Hymenoptera: Pteromalidae) toward the hemocytes of their natural hosts, non-target insects and cultured insect cells. Toxicon, 46(3), 337-349."]}</dct:description>
    <dct:accessRights rdf:resource="http://publications.europa.eu/resource/authority/access-right/PUBLIC"/>
    <dct:accessRights>
      <dct:RightsStatement rdf:about="info:eu-repo/semantics/openAccess">
        <rdfs:label>Open Access</rdfs:label>
      </dct:RightsStatement>
    </dct:accessRights>
    <dct:license rdf:resource="https://creativecommons.org/licenses/by-sa/4.0/legalcode"/>
    <dcat:distribution>
      <dcat:Distribution>
        <dcat:accessURL rdf:resource="https://doi.org/10.5281/zenodo.1064667"/>
        <dcat:byteSize>166442</dcat:byteSize>
        <dcat:downloadURL rdf:resource="https://zenodo.org/record/1064667/files/AES 22 Manzoor et al.pdf"/>
        <dcat:mediaType>application/pdf</dcat:mediaType>
      </dcat:Distribution>
    </dcat:distribution>
  </rdf:Description>
</rdf:RDF>
79
54
views
downloads
All versions This version
Views 7979
Downloads 5454
Data volume 9.0 MB9.0 MB
Unique views 6464
Unique downloads 5050

Share

Cite as