Journal article Open Access

FUNCTIONAL ANALYSIS OF BRACON HEBETOR VENOM ON TARGET AND NON-TARGET INSECT CELL LINES

Manzoor Atif; Abdin Ul Zain; Shaina Hoor; Webb A. Bruce; Jamil Amer


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{
  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.1064667", 
  "container_title": "Acta entomologica Serbica", 
  "language": "eng", 
  "title": "FUNCTIONAL ANALYSIS OF BRACON HEBETOR VENOM ON TARGET AND NON-TARGET INSECT CELL LINES", 
  "issued": {
    "date-parts": [
      [
        2017, 
        11, 
        22
      ]
    ]
  }, 
  "abstract": "<p>Insect cell cultures are widely used in studies of insect cell physiology, toxicology, developmental biology and microbial pathology. The lethal effects of crude venom extracted from the ectoparasitic wasp <em>Bracon hebetor </em>were examined with three cultured insect cell lines; <em>Spodoptera frugiperda </em>(Sf9), <em>Tribolium castaneum </em>(TcA) and <em>Aedes aegypti </em>(Aag-2). Venom caused cells to round-up, swell and eventually die. Despite similar sensitivities and overlapping LC<sub>50</sub> values [(0.00125-0.00695) venom reservoir equivalents (VRE)/&micro;l], significant differences were noted at the onset of cytotoxicity among the three insect cell lines. Cells from the <em>Tribolium castaneum </em>(TcA) and <em>Aedes aegypti </em>(Aag-2) showed little sensitivity to the venom: 0.0046 VRE were needed to induce 50% mortality in TcA [50% lethal concentration (LC<sub>50</sub>) = 0.0046 VRE/&micro;l], and 0.0069 VRE were needed to induce 50% mortality in Aag-2 [50% lethal concentration (LC<sub>50</sub>) = 0.0069 VRE/&micro;l). Over 80% of the Sf9 cells were nonviable within 1 h after the addition of an LC<sub>99</sub> dose of venom, whereas the other cells required a 5-10-fold longer incubation period to produce mortality above 50%.</p>", 
  "author": [
    {
      "family": "Manzoor Atif"
    }, 
    {
      "family": "Abdin Ul Zain"
    }, 
    {
      "family": "Shaina Hoor"
    }, 
    {
      "family": "Webb A. Bruce"
    }, 
    {
      "family": "Jamil Amer"
    }
  ], 
  "page": "103-111", 
  "volume": "22", 
  "type": "article-journal", 
  "id": "1064667"
}
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