1058725
doi
10.5281/zenodo.1058725
oai:zenodo.org:1058725
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Direct and Indirect Somatic Embryogenesis from Petiole and Leaf Explants of Purple Fan Flower (Scaevola aemula R. Br. cv. 'Purple Fanfare')
Shyama Ranjani Weerakoon
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Creative Commons Attribution 4.0 International
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Petiole and leaf explants
Scaevola aemula
Somaticembryogenesis
Direct and indirect somatic embryogenesis (SE) from
petiole and leaf explants of Scaevola aemula R. Br. cv. 'Purple
Fanfare' was achieved. High frequency of somatic embryos was
obtained directly from petiole and leaf explants using an inductive
plant growth regulator signal thidiazuron (TDZ). Petiole explants
were more responsive to SE than leaves. Plants derived from somatic
embryos of petiole explants germinated more readily into plants. SE
occurred more efficiently in half-strength Murashige and Skoog
(MS) medium than in full-strength MS medium. Non-embryogenic
callus induced by 2, 4-dichlorophenoxyacetic acid was used to
investigate the feasibility of obtaining SE with TDZ as a secondary
inductive plant growth regulator (PGR) signal. Non-embryogenic
callus of S. aemula was able to convert into an "embryogenic
competent mode" with PGR signal. Protocol developed for induction
of direct and indirect somatic embryogenesis in S. aemula can
improve the large scale propagation system of the plant in future.
Zenodo
2010-05-21
info:eu-repo/semantics/article
1058724
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3225
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286330
md5:d25ae9a4a7a47df336310873b4e51149
https://zenodo.org/records/1058725/files/3225.pdf
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10.5281/zenodo.1058724
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doi