The Tyrosinase and Cyclooxygenase Inhibitory Activities and Cytotoxicity Screening of Tamarindus indica Seeds
The methanolic extracts from seeds of tamarind
(Tamarindus indica) was prepared by Soxhlet apparatus extraction
and evaluated for total phenolic content by Folin-Ciocalteu method.
Then, methanolic extract was screened biological activities (In vitro)
for anti-melanogenic activity by tyrosinase inhibition test, antiinflammation
activity by cyclooxygenase 1 (COX-1) and
cyclooxygenase 2 (COX-2) inhibition test, and cytotoxic screening
test with Vero cells. The results showed that total phenolic content,
which contained in extract, was contained 27.72 mg of gallic acid
equivalent per g of dry weight. The ability to inhibit tyrosinase
enzyme, which exerted by Tamarind seed extracts (1 mg/ml) was
52.13 ± 0.42 %. The extract was not possessed inhibitory effect to
COX-1 and COX-2 enzymes and cytotoxic effect to Vero cells. The
finding is concludes that tested seed extract was possessed
antimelanogenic activity with non-toxic effects. However, there was
not exhibited anti-inflammatory activity. Further studies include the
use of advance biological models to confirm this biological activity,
as well as, the isolation and characterization of the purified
compounds that it was contained.
D. Jang, B. Lee, C. Jeon et al, "Melanogenesis inhibitor form Paper
Mulberry," Cosmetics & Toiletries Magazine, vol. 112, 1997, pp. 59-62.
M. De, A. Krishna and A.B. Baneerjee , "Antimicrobial screening of
some Indian spices," Phytotherapy Research, vol. 13, 1999, pp. 616-
 Y. Sudjaroen, R. Haubner, G. Wurtele, W.E. Hull, G. Erben, B.
Spiegelhalder, S. Changbumrung, H. Bartsch and R.W. Owen, "Isolation
and structure elucidation of phenolic antioxidants from Tamarind
(Tamarindus indica L.) seeds and pericarp," Food and Chemical
Toxicology, vol. 43, 2005, pp. 1673-1682.
 V.L. Singleton, R. Orthofer and R.M. Lamuela-Ravent├│s, "Analysis of
Total Phenols and Other Oxidation Substrates and Antioxidants by
Means of Folin-ciocalteu Reagent," Methods in Enzymology, vol. 299,
1999, pp. 152-178.
 V. J. Hearing and K. Tsukamoto, "Enzymatic control of pigmentation in
mammals," FASEB J., vol. 5, Nov. 1991, pp. 2902ÔÇö2909.
 Y.J. Kim, "Antimelanogenic and antioxidant properties of gallic acid,"
Biol. Pharm. Bull. vol. 30, Jun. 2007, pp. 1052ÔÇö1055.
 K. Kirtikara, S.G. Morham, R. Raghow, S.J.F. Laulederkind, T.
Kanekura, S. Goorha and L.R. Ballou, "Compensatory protaglandin E2
biosynthesis in cyclooxygenase1 or 2 null cells," J Exp Med, vol. 187,
1998, pp. 517-23.
 K. Kirtikara, S. Swangkul and L.R. Ballou, "The analysis of
nonsteroidal anti-inflammatory drug selectivity in prostaglandin G/H
synthase (PGHS)-null cells," Inflamm Res, vol. 50, 2001, pp. 327-32.
 P. Skehan, R. Storeng, D. Scudiero, et al, "New colorimetric cytotoxicity
assay for anticancer-drug screening," J Natl Cancer Inst, vol. 82, 1990,
 M. Naczk and F. Shahidi, "Phenolics in cereals, fruits and vegetables:
occurrence, extraction and analysis," J Pharm Biomed Anal, vol. 41, Jan.
2006, pp. 1523-1542.
 H-J. Yoon and S.J. Baek, "Molecular targets of dietary polyphenols with
anti-inflammatory properties," Yonsei Med J, vol. 46, 2005, pp. 585-596.
O. Lee, H. Kang and S. Han, "Oriental herbs in cosmetics," Cosmetics &
Toiletries Magazine, vol. 112, Jan. 1997, pp. 57-64.
P. L. Olliaro and Y. Yuthavong, "An overview of chemotherapeutic
targets for antimalarial drug discovery," Pharmacol Ther, vol. 81, 1999,
S. Briganti, E. Camera, and M. Picado, "Chemical and instrumental
approachs to treat hyperpigmentation," Pigment Cell Res, vol. 16, 2003,
T, Phakruschaphan, "Comparison of peeling and extraction methods in
the production of Tamarind seed gum," The Kasetsart Journal of
Natural Sciences, vol. 16, 1982, pp. 74-81.
T. Piamphongsant, "Problems in developing cosmetic in dustry," in
Conf. 2003 Aug, 14 The development of cosmetic industry for selfreliance"
organized by the office of the National Research Council of
T. Tsuda, M. Watanabe, K. Ohshima, A. Yamamoto, S. Kawakishi and
T. Osawa, "Antioxidative components isolated from the seed of
Tamarind (Tamarindus indica L.)," Journal Agricultural and Food
Chemistry, vol. 42, 1994, pp. 2671-2674.
T. Tsuda, T. Osawa, Y. Makino, H. Kato and S. Kawakishi, "Screening
for antioxidant activity of edible pulses," Bioscientific Biotechnological
Biochemistry, vol. 57, 1993, pp. 1606-1608.