10.5281/zenodo.1057039
https://zenodo.org/records/1057039
oai:zenodo.org:1057039
Sarkar M. A. Kawsar
Sarkar M. A. Kawsar
Sarkar M. A. Mamun
Sarkar M. A. Mamun
Md S. Rahman
Md S. Rahman
Hidetaro Yasumitsu
Hidetaro Yasumitsu
Yasuhiro Ozeki
Yasuhiro Ozeki
Biological Effects of a Carbohydrate-Binding Protein from an Annelid, Perinereis nuntia Against Human and Phytopathogenic Microorganisms
Zenodo
2010
Perinereis nuntia
Lectin
Inhibition zone
Mycelial growth.
2010-01-21
eng
10.5281/zenodo.1057038
https://zenodo.org/communities/waset
2345
Creative Commons Attribution 4.0 International
Lectins have a good scope in current clinical
microbiology research. In the present study evaluated the
antimicrobial activities of a D-galactose binding lectin (PnL) was
purified from the annelid, Perinereis nuntia (polychaeta) by affinity
chromatography. The molecular mass of the lectin was determined to
be 32 kDa as a single polypeptide by SDS-PAGE under both reducing
and non-reducing conditions. The hemagglutinating activity of the
PnL showed against trypsinized and glutaraldehyde-fixed human
erythrocytes was specifically inhibited by D-Gal, GalNAc,
Galβ1-4Glc and Galα1-6Glc. PnL was evaluated for in vitro
antibacterial screening studies against 11 gram-positive and
gram-negative microorganisms. From the screening results, it was
revealed that PnL exhibited significant antibacterial activity against
gram-positive bacteria. Bacillus megaterium showed the highest
growth inhibition by the lectin (250 μg/disc). However, PnL did not
inhibit the growth of gram-negative bacteria such as Vibrio cholerae
and Pseudomonas sp. PnL was also examined for in vitro antifungal
activity against six fungal phytopathogens. PnL (100 μg/mL) inhibited
the mycelial growth of Alternaria alternata (24.4%). These results
indicate that future findings of lectin applications obtained from
annelids may be of importance to life sciences.