November 6, 2017 Dataset Open Access

Bernard, Abram R; Jessop, T. Carson; Kumar, Prashant; Dickenson, Nicholas E.

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    <subfield code="a">&lt;p&gt;The binding affinity between each of the engineered IpaD alanine mutants and the stable IpaB&lt;sup&gt;28-226&lt;/sup&gt; construct was measured using fluorescence polarization. Here, the DOC effect on binding affinity between IpaB and the engineered IpaD π-helix mutants was quantified by holding IpaB&lt;sup&gt;28-226&lt;/sup&gt;-Alexa568 concentration constant  while the concentration of IpaD or engineered IpaD mutant was titrated from 0-10 μM with identical conditions then tested in the presence of 1 mM DOC.&lt;/p&gt;</subfield>
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