November 6, 2017 Dataset Open Access

Bernard, Abram R; Jessop, T. Carson; Kumar, Prashant; Dickenson, Nicholas E.

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{
  "inLanguage": {
    "alternateName": "eng", 
    "@type": "Language", 
    "name": "English"
  }, 
  "description": "<p>The binding affinity between each of the engineered IpaD alanine mutants and the stable IpaB<sup>28-226</sup> construct was measured using fluorescence polarization.\u00a0Here, the DOC effect on binding affinity between IpaB and the engineered IpaD \u03c0-helix mutants was quantified by holding IpaB<sup>28-226</sup>-Alexa568 concentration constant\u00a0 while the concentration of IpaD or engineered IpaD mutant was titrated from 0-10 \u03bcM with identical conditions then tested in the presence of 1 mM DOC.</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Department of Chemistry and Biochemistry, Utah State University", 
      "@id": "https://orcid.org/0000-0001-7681-7728", 
      "@type": "Person", 
      "name": "Bernard, Abram R"
    }, 
    {
      "affiliation": "Department of Chemistry and Biochemistry, Utah State University", 
      "@type": "Person", 
      "name": "Jessop, T. Carson"
    }, 
    {
      "affiliation": "Department of Pharmaceutical Chemistry, University of Kansas,", 
      "@id": "https://orcid.org/0000-0002-4959-3156", 
      "@type": "Person", 
      "name": "Kumar, Prashant"
    }, 
    {
      "affiliation": "Department of Chemistry and Biochemistry, Utah State University", 
      "@id": "https://orcid.org/0000-0003-1572-6077", 
      "@type": "Person", 
      "name": "Dickenson, Nicholas E."
    }
  ], 
  "url": "https://zenodo.org/record/1042802", 
  "datePublished": "2017-11-06", 
  "@context": "https://schema.org/", 
  "distribution": [
    {
      "contentUrl": "https://zenodo.org/api/files/57b4ee4b-e535-46f5-b6a8-7eaa69612c90/IpaB IpaD polarization data.csv", 
      "encodingFormat": "csv", 
      "@type": "DataDownload"
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  "identifier": "https://doi.org/10.5281/zenodo.1042802", 
  "@id": "https://doi.org/10.5281/zenodo.1042802", 
  "@type": "Dataset", 
  "name": "Polarization measurements of DOC-dependent IpaB-IpaD interactions"
}