November 6, 2017 Dataset Open Access

Bernard, Abram R; Jessop, T. Carson; Kumar, Prashant; Dickenson, Nicholas E.

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    "description": "<p>The binding affinity between each of the engineered IpaD alanine mutants and the stable IpaB<sup>28-226</sup> construct was measured using fluorescence polarization.\u00a0Here, the DOC effect on binding affinity between IpaB and the engineered IpaD \u03c0-helix mutants was quantified by holding IpaB<sup>28-226</sup>-Alexa568 concentration constant\u00a0 while the concentration of IpaD or engineered IpaD mutant was titrated from 0-10 \u03bcM with identical conditions then tested in the presence of 1 mM DOC.</p>", 
    "language": "eng", 
    "title": "Polarization measurements of DOC-dependent IpaB-IpaD interactions", 
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        "affiliation": "Department of Chemistry and Biochemistry, Utah State University", 
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        "affiliation": "Department of Chemistry and Biochemistry, Utah State University", 
        "name": "Jessop, T. Carson"
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        "affiliation": "Department of Pharmaceutical Chemistry, University of Kansas,", 
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        "affiliation": "Department of Chemistry and Biochemistry, Utah State University", 
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