November 6, 2017 Dataset Open Access

Bernard, Abram R; Jessop, T. Carson; Kumar, Prashant; Dickenson, Nicholas E.

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  <identifier identifierType="DOI">10.5281/zenodo.1042802</identifier>
  <creators>
    <creator>
      <creatorName>Bernard, Abram R</creatorName>
      <givenName>Abram R</givenName>
      <familyName>Bernard</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0001-7681-7728</nameIdentifier>
      <affiliation>Department of Chemistry and Biochemistry, Utah State University</affiliation>
    </creator>
    <creator>
      <creatorName>Jessop, T. Carson</creatorName>
      <givenName>T. Carson</givenName>
      <familyName>Jessop</familyName>
      <affiliation>Department of Chemistry and Biochemistry, Utah State University</affiliation>
    </creator>
    <creator>
      <creatorName>Kumar, Prashant</creatorName>
      <givenName>Prashant</givenName>
      <familyName>Kumar</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-4959-3156</nameIdentifier>
      <affiliation>Department of Pharmaceutical Chemistry, University of Kansas,</affiliation>
    </creator>
    <creator>
      <creatorName>Dickenson, Nicholas E.</creatorName>
      <givenName>Nicholas E.</givenName>
      <familyName>Dickenson</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-1572-6077</nameIdentifier>
      <affiliation>Department of Chemistry and Biochemistry, Utah State University</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Polarization measurements of DOC-dependent IpaB-IpaD interactions</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2017</publicationYear>
  <dates>
    <date dateType="Issued">2017-11-06</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Dataset"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/1042802</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.1042801</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;The binding affinity between each of the engineered IpaD alanine mutants and the stable IpaB&lt;sup&gt;28-226&lt;/sup&gt; construct was measured using fluorescence polarization. Here, the DOC effect on binding affinity between IpaB and the engineered IpaD π-helix mutants was quantified by holding IpaB&lt;sup&gt;28-226&lt;/sup&gt;-Alexa568 concentration constant  while the concentration of IpaD or engineered IpaD mutant was titrated from 0-10 μM with identical conditions then tested in the presence of 1 mM DOC.&lt;/p&gt;</description>
  </descriptions>
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