November 6, 2017 Dataset Open Access

Bernard, Abram R; Jessop, T. Carson; Kumar, Prashant; Dickenson, Nicholas E.

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{
  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.1042802", 
  "language": "eng", 
  "title": "Polarization measurements of DOC-dependent IpaB-IpaD interactions", 
  "issued": {
    "date-parts": [
      [
        2017, 
        11, 
        6
      ]
    ]
  }, 
  "abstract": "<p>The binding affinity between each of the engineered IpaD alanine mutants and the stable IpaB<sup>28-226</sup> construct was measured using fluorescence polarization.\u00a0Here, the DOC effect on binding affinity between IpaB and the engineered IpaD \u03c0-helix mutants was quantified by holding IpaB<sup>28-226</sup>-Alexa568 concentration constant\u00a0 while the concentration of IpaD or engineered IpaD mutant was titrated from 0-10 \u03bcM with identical conditions then tested in the presence of 1 mM DOC.</p>", 
  "author": [
    {
      "family": "Bernard, Abram R"
    }, 
    {
      "family": "Jessop, T. Carson"
    }, 
    {
      "family": "Kumar, Prashant"
    }, 
    {
      "family": "Dickenson, Nicholas E."
    }
  ], 
  "type": "dataset", 
  "id": "1042802"
}