Project deliverable Open Access

EM quality assurance workflow

del Cano, Laura

The image end result of a cryo-EM study is a three-dimensional density map in which the different parts of the volume may present different degrees of quality (resolution) as a result of, most typically, flexibility or compositional differences of the macromolecule under consideration. Consequently, maps are better described using the concept of local (per voxel) resolution, rather than by a single global value. However, the concept of what resolution truly means is not simple, and several approaches have been proposed to measure resolution (for a review on resolution measures in cryo-EM, see 1).

The existence of different methods to calculate resolution, each included in different software packages, provided a very good case for Scipion Web Tools, a web application built over Scipion framework software that offers access to predefined image processing workflows over the web.

Files (382.0 kB)
Name Size
382.0 kB Download
All versions This version
Views 1717
Downloads 88
Data volume 3.1 MB3.1 MB
Unique views 1616
Unique downloads 88


Cite as