1036490
doi
10.5281/zenodo.1036490
oai:zenodo.org:1036490
user-iajpr
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF IVERMECTIN AND CLORSULON IN IVERCAM INJECTION
Vegad Kunjal L*, Paranjape Dipty B., Shah Dhwani A., Patel Ekta D., Patel Yogesh K., Patel Kaushik R.
Sharda School of Pharmacy, Gandhinagar, Pethapur.
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
RP-HPLC, Ivermectin, Clorsulon, Ivercam Injection.
<p>A precise, simple, accurate and selective method was developed and validate for estimation of Ivermectin and Clorsulon in Ivercam injection, Reversed phase high performance liquid chromatographic (RP-HPLC) method was developed for routine quantification of Ivermectin and Clorsulon in laboratory prepared mixtures as well as in combined dosage form. Chromatographic separation was achieved on a BDS hypersil C18 (5μ, 250 x 4.6 mm) utilizing mobile phase of filtered and degassed mixture of 60 phosphate buffer (pH 5.5 adjusted with 1% O-phosphoric acid) and Methanol (60:40 v/v) at a flow rate of 1 mL/min with UV detection at 234 nm. The method has been validated for linearity, accuracy and precision. In RP-HPLC method, the calibration graphs were linear in the concentration range of 2.5-7.5 μg/ml for Ivermectin and 25-75 μg/ml for Clorsulon with percentage recoveries of 100.34 % and 99.76% for Ivermectin and Clorsulon respectively. Conclusion: The results obtained by RP-HPLC methods are rapid, accurate and precise. Therefore proposed method can be used for routine analysis of Clorsulon and Ivermectin in injection.</p>
Zenodo
2017-08-30
info:eu-repo/semantics/article
1036489
user-iajpr
1579540920.350824
795524
md5:fe476f8eb9c80aba8a875c778f4f9a23
https://zenodo.org/records/1036490/files/15.pdf
public
10.5281/zenodo.1036489
isVersionOf
doi
INDO AMERICAN JOURNAL OF PHARMACEUTICAL RESEARCH
07
09
523-531
2017-08-30