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DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF IVERMECTIN AND CLORSULON IN IVERCAM INJECTION

Vegad Kunjal L*, Paranjape Dipty B., Shah Dhwani A., Patel Ekta D., Patel Yogesh K., Patel Kaushik R.


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{
  "description": "<p>A precise, simple, accurate and selective method was developed and validate for estimation of Ivermectin and Clorsulon in Ivercam injection, Reversed phase high performance liquid chromatographic (RP-HPLC) method was developed for routine quantification of Ivermectin and Clorsulon in laboratory prepared mixtures as well as in combined dosage form. Chromatographic separation was achieved on a BDS hypersil C18 (5\u03bc, 250 x 4.6 mm) utilizing mobile phase of filtered and degassed mixture of 60 phosphate buffer (pH 5.5 adjusted with 1% O-phosphoric acid) and Methanol (60:40 v/v) at a flow rate of 1 mL/min with UV detection at 234 nm. The method has been validated for linearity, accuracy and precision. In RP-HPLC method, the calibration graphs were linear in the concentration range of 2.5-7.5 \u03bcg/ml for Ivermectin and 25-75 \u03bcg/ml for Clorsulon with percentage recoveries of 100.34 % and 99.76% for Ivermectin and Clorsulon respectively. Conclusion: The results obtained by RP-HPLC methods are rapid, accurate and precise. Therefore proposed method can be used for routine analysis of Clorsulon and Ivermectin in injection.</p>", 
  "license": "http://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Sharda School of Pharmacy, Gandhinagar, Pethapur.", 
      "@type": "Person", 
      "name": "Vegad Kunjal L*, Paranjape Dipty B., Shah Dhwani A., Patel Ekta D., Patel Yogesh K., Patel Kaushik R."
    }
  ], 
  "url": "https://zenodo.org/record/1036490", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2017-08-30", 
  "headline": "DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF IVERMECTIN AND CLORSULON IN IVERCAM INJECTION", 
  "keywords": [
    "RP-HPLC, Ivermectin, Clorsulon, Ivercam Injection."
  ], 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.5281/zenodo.1036490", 
  "@id": "https://doi.org/10.5281/zenodo.1036490", 
  "@type": "ScholarlyArticle", 
  "name": "DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF IVERMECTIN AND CLORSULON IN IVERCAM INJECTION"
}
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