August 30, 2017 Journal article Open Access

Vegad Kunjal L*, Paranjape Dipty B., Shah Dhwani A., Patel Ekta D., Patel Yogesh K., Patel Kaushik R.

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        <foaf:name>Vegad Kunjal L*, Paranjape Dipty B., Shah Dhwani A., Patel Ekta D., Patel Yogesh K., Patel Kaushik R.</foaf:name>
        <foaf:givenName>Paranjape Dipty B., Shah Dhwani A., Patel Ekta D., Patel Yogesh K., Patel Kaushik R.</foaf:givenName>
        <foaf:familyName>Vegad Kunjal L*</foaf:familyName>
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            <foaf:name>Sharda School of Pharmacy, Gandhinagar, Pethapur.</foaf:name>
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    <dct:title>DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF IVERMECTIN AND CLORSULON IN IVERCAM INJECTION</dct:title>
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    <dct:issued rdf:datatype="http://www.w3.org/2001/XMLSchema#gYear">2017</dct:issued>
    <dcat:keyword>RP-HPLC, Ivermectin, Clorsulon, Ivercam Injection.</dcat:keyword>
    <dct:issued rdf:datatype="http://www.w3.org/2001/XMLSchema#date">2017-08-30</dct:issued>
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    <dct:description>&lt;p&gt;A precise, simple, accurate and selective method was developed and validate for estimation of Ivermectin and Clorsulon in Ivercam injection, Reversed phase high performance liquid chromatographic (RP-HPLC) method was developed for routine quantification of Ivermectin and Clorsulon in laboratory prepared mixtures as well as in combined dosage form. Chromatographic separation was achieved on a BDS hypersil C18 (5μ, 250 x 4.6 mm) utilizing mobile phase of filtered and degassed mixture of 60 phosphate buffer (pH 5.5 adjusted with 1% O-phosphoric acid) and Methanol (60:40 v/v) at a flow rate of 1 mL/min with UV detection at 234 nm. The method has been validated for linearity, accuracy and precision. In RP-HPLC method, the calibration graphs were linear in the concentration range of 2.5-7.5 μg/ml for Ivermectin and 25-75 μg/ml for Clorsulon with percentage recoveries of 100.34 % and 99.76% for Ivermectin and Clorsulon respectively. Conclusion: The results obtained by RP-HPLC methods are rapid, accurate and precise. Therefore proposed method can be used for routine analysis of Clorsulon and Ivermectin in injection.&lt;/p&gt;</dct:description>
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