Journal article Open Access

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF IVERMECTIN AND CLORSULON IN IVERCAM INJECTION

Vegad Kunjal L*, Paranjape Dipty B., Shah Dhwani A., Patel Ekta D., Patel Yogesh K., Patel Kaushik R.


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{
  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.1036490", 
  "container_title": "INDO AMERICAN JOURNAL OF PHARMACEUTICAL RESEARCH", 
  "title": "DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF IVERMECTIN AND CLORSULON IN IVERCAM INJECTION", 
  "issued": {
    "date-parts": [
      [
        2017, 
        8, 
        30
      ]
    ]
  }, 
  "abstract": "<p>A precise, simple, accurate and selective method was developed and validate for estimation of Ivermectin and Clorsulon in Ivercam injection, Reversed phase high performance liquid chromatographic (RP-HPLC) method was developed for routine quantification of Ivermectin and Clorsulon in laboratory prepared mixtures as well as in combined dosage form. Chromatographic separation was achieved on a BDS hypersil C18 (5\u03bc, 250 x 4.6 mm) utilizing mobile phase of filtered and degassed mixture of 60 phosphate buffer (pH 5.5 adjusted with 1% O-phosphoric acid) and Methanol (60:40 v/v) at a flow rate of 1 mL/min with UV detection at 234 nm. The method has been validated for linearity, accuracy and precision. In RP-HPLC method, the calibration graphs were linear in the concentration range of 2.5-7.5 \u03bcg/ml for Ivermectin and 25-75 \u03bcg/ml for Clorsulon with percentage recoveries of 100.34 % and 99.76% for Ivermectin and Clorsulon respectively. Conclusion: The results obtained by RP-HPLC methods are rapid, accurate and precise. Therefore proposed method can be used for routine analysis of Clorsulon and Ivermectin in injection.</p>", 
  "author": [
    {
      "family": "Vegad Kunjal L*, Paranjape Dipty B., Shah Dhwani A., Patel Ekta D., Patel Yogesh K., Patel Kaushik R."
    }
  ], 
  "page": "523-531", 
  "volume": "07", 
  "type": "article-journal", 
  "issue": "09", 
  "id": "1036490"
}
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