Journal article Open Access

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF SOFOSBUVIR AND DACLATASVIR DRUG PRODUCT BY RP-HPLC METHOD

Benzil Dudekula1*, Dr.C.Ramachandraiah2, Dr.N.Devanna3


MARC21 XML Export

<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
  <leader>00000nam##2200000uu#4500</leader>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Sofosbuvir And Daclatasvir, Isocratic, HPLC, C18, Trifluoro Acetic Acid, Methanol, Acetonitrile And Validation.</subfield>
  </datafield>
  <controlfield tag="005">20190409131639.0</controlfield>
  <controlfield tag="001">1036476</controlfield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">698776</subfield>
    <subfield code="z">md5:e9e0c8b074752d349a488ff66a17cbb7</subfield>
    <subfield code="u">https://zenodo.org/record/1036476/files/11.pdf</subfield>
  </datafield>
  <datafield tag="542" ind1=" " ind2=" ">
    <subfield code="l">open</subfield>
  </datafield>
  <datafield tag="260" ind1=" " ind2=" ">
    <subfield code="c">2017-08-30</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="O">
    <subfield code="p">openaire</subfield>
    <subfield code="p">user-iajpr</subfield>
    <subfield code="o">oai:zenodo.org:1036476</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="4">
    <subfield code="c">480-487</subfield>
    <subfield code="n">09</subfield>
    <subfield code="p">INDO AMERICAN JOURNAL OF PHARMACEUTICAL RESEARCH</subfield>
    <subfield code="v">07</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="u">1S.V.Engineering college for women, Tirupati-517507, Chittor (Dt). 2SKIT, Srikalahasthi-517640. 3Jawaharlal Nehru Technological University Anantapur, College of Engineering, Anantapur-515002, Andhra Pradesh.</subfield>
    <subfield code="a">Benzil Dudekula1*, Dr.C.Ramachandraiah2, Dr.N.Devanna3</subfield>
  </datafield>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF SOFOSBUVIR AND DACLATASVIR DRUG PRODUCT BY RP-HPLC METHOD</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">user-iajpr</subfield>
  </datafield>
  <datafield tag="540" ind1=" " ind2=" ">
    <subfield code="u">http://creativecommons.org/licenses/by/4.0/legalcode</subfield>
    <subfield code="a">Creative Commons Attribution 4.0 International</subfield>
  </datafield>
  <datafield tag="650" ind1="1" ind2="7">
    <subfield code="a">cc-by</subfield>
    <subfield code="2">opendefinition.org</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">&lt;p&gt;Analytical method was developed for the estimation of Sofosbuvir and Daclatasvir drug substance by liquid chromatography. The chromatographic separation was achieved on C18 column (XTerra RP18 150*4.6, 5um) at ambient temperature .The separation achieved employing a mobile phase consists of 0.1%v/v Trifluoro acetic acid in water: Acetonitrile (60:40). The flow rate was 1.0 ml/ minute and ultra violet detector at 275nm. The average retention time for Sofosbuvir and Daclatasvir found to be 2.09 and 3.50 min. The proposed method was validated for selectivity, precision, linearity and accuracy. All validation parameters were within the acceptable range. The assay methods were found to be linear from 80-240 μg/ml for Sofosbuvir and 12-36μg/ml for Daclatasvir.&lt;/p&gt;</subfield>
  </datafield>
  <datafield tag="773" ind1=" " ind2=" ">
    <subfield code="n">doi</subfield>
    <subfield code="i">isVersionOf</subfield>
    <subfield code="a">10.5281/zenodo.1036475</subfield>
  </datafield>
  <datafield tag="024" ind1=" " ind2=" ">
    <subfield code="a">10.5281/zenodo.1036476</subfield>
    <subfield code="2">doi</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">publication</subfield>
    <subfield code="b">article</subfield>
  </datafield>
</record>
38
143
views
downloads
All versions This version
Views 3838
Downloads 143143
Data volume 99.9 MB99.9 MB
Unique views 3838
Unique downloads 121121

Share

Cite as