EXPLORATION OF VARIOUS CLASSICAL AND CHEMOMETRIC ASSISTED UV SPECTROPHOTOMETRIC METHODS FOR ESTIMATION OF CHLORHEXIDINE GLUCONATE AND CETRIMIDE IN BULK AND ITS FORMULATION

The present work inculcates with the approach for development of various classical and chemometrics assisted UV spectrophotometric methods for estimation of Chlorhexidine gluconate (CH) and Cetrimide (CET) in bulk and its formulation. The methods developed herein include simple Simultaneous equation method (Vieordt’s method), First Derivative spectroscopy method, Multicomponent analysis method, Classical least squares, Inverse least square, Partial least squares, Absorption ratio spectra method and Mean centering of ratio spectra method. The developed methods were successfully validated according to ICH Q2 (R1) guidelines. All methods showed a good linear response. The mean percentage assay values for all UV methods were found to be in the range of 98-102%. Statistical analysis was also applied for assay results of the developed methods which included One-way ANOVA and post hoc analysis like Tukey Honest significant test and Scheffe multiple comparison test. The major outcome of research imbibes to be developed analytical methods were found to be specific, selective, and robust and can be applied for routine analysis of marketed formulation in laboratory premises. Thus, various novel and simple UV analytical methods were explored and available for analysis of the selected drugs.