SIMULTANEOUS ESTIMATION OF AMBROXOL, CHLORPHENERAMINE MALEATE AND GUAIPHENESIN IN BULK AND LIQUID DOSAGE FORM BY CHEMOMETRIC ASSISTED SPECTROPHOTOMETRIC METHODS.

A simple UV-visible spectroscopic method was developed and Chemometric designs were applied for the simultaneous estimation of Ambroxol (AMB), Chlorpheneramine maleate (CPM) and Guaiphenesin (GPN) in bulk and liquid dosage form. The spectroscopic method was developed by using methanol as solvent for the three drugs and the data generated from the spectra were mined by using Chemometric methods such as trilinear regression analysis, Cramer’s matrix method, Method of least squares, Multivariate calibration methods such as partial least square regression(PLS) and Principle component regression(PCR). The wavelengths selected for all the above methods were 248 nm (wavelength of maximum absorption; λmax of AMB), 261 nm (wavelength of maximum absorption; λmax of CPM) and 274 nm (wavelength of maximum absorption; λmax of GPN). Results: The methods hold good linearity for AMB from 10-30 μg/ml, for CPM from 2-10 μg/ml and GPN from 10-80 μg/ml with regression coefficient values of 0.999, 0.998 and 0.999 respectively. The intraday and inter-day precision was found to be less than 2% RSD. The percentage recovery and percentage assay was in the range of 95-105% for Ambroxol (AMB), Chlorpheneramine maleate (CPM) and Guaiphenesin (GPN) by all the methods. Conclusion: The developed methods neither require any cumbersome separation procedure nor complex derivatization procedures for the analysis of the three drugs and moreover they are effective in minimizing the errors in analysis, simple and economical.