Journal article Open Access

EVALUATION OF MOMORDICA DIOICA SEED EXTRACT FORALPHA AMYLASE INHIBITORY ACTIVITY AND ANTIOXIDANT POTENTIAL IN RAT MODEL

P Sailaja Rao1*, G. Krishna Mohan2

The effect of ethylacetate extract of Momordica dioica seeds by in-vitro alpha amylase assay and in vivo anti-oxidant potential in streptozotocin induced diabetic rats was studied. Type II diabetes was induced in Wistar rats by a single intraperitoneal injection of streptozotocin (STZ) at the dose of 30 mg/kg body weight. In vitro α-amylase activity was performed by using dinitrosalicylic acid. Experiment for in vivo antioxidant effect was carried out after a continuous treatment with ethylacetate extract for a period of 15 days at the dose of 100 and 200 mg/kg body weight. Serum glucose levels were estimated by GOD-POD method, and ethylacetate extract of Momordica dioica seeds at the dose of 200 mg/kg body weight showed significant reduction in serum glucose levels at 2hrs, 4 hrs, 6 hrs and 8 hrs respectively in STZ-induced diabetic rats (ap<0.001). In in vitro α-amylase assay, there was a significant inhibition with IC50 of 58±0.091 μg/ml in comparable to the standard Acarbose. Treatment for a period of 15 days showed significant elevation of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). ((ap<0.001). The results revealed that the Momordica dioica seeds proved to possess potent α-amylase, anti-diabetic and antioxidant activities.

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