Published August 5, 2017
| Version v1
Journal article
Open
Proteome profiling of s-nitrosylated synaptosomal proteins by isobaric mass tags.
Creators
- 1. German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany
- 2. Institute of Biochemistry and Molecular Biology, University of Bonn, Germany
- 3. Department of Neurodegenerative Diseases & Gerontopsychiatry, University Hospital Bonn, Bonn, Germany
- 4. German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany and Department of Neurodegenerative Diseases & Gerontopsychiatry, University Hospital Bonn, Bonn, Germany
Description
Short communication
Highlights
• Protocol for quantitative proteomics of nitrosylation on synaptosomal proteins.
• Identification of endogenous nitrosylation independent of induction by NO donors.
• Use of iodoTMT sixplex mass tags for stable labeling, enrichment, identification, and multiplex quantitation.
• Applicable on low amounts of sample material of mouse and human brain tissue.
Files
Wijasa_JNeurosciMeth_2017-P21.pdf
Files
(1.5 MB)
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