Journal article Open Access

Antimicrobial packaging based on E-polylysine bioactive film for the control of mycotoxigenic fungi in vitro and in bread

C. Luz1 | J. Calpe1 | F. Saladino1 | Fernando B. Luciano2 | M. Fernandez-Franzon1 | J. Manes1 | G. Meca1


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        <foaf:name>C. Luz1 | J. Calpe1 | F. Saladino1 | Fernando B. Luciano2 | M. Fernandez-Franzon1 | J. Manes1 | G. Meca1</foaf:name>
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    <dct:title>Antimicrobial packaging based on E-polylysine bioactive film for the control of mycotoxigenic fungi in vitro and in bread</dct:title>
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    <dct:description>&lt;p&gt;E-Poly-L-lysine (E-PL) is a cationic peptide with a broad-spectrum antimicrobial activity. This study&lt;br&gt; investigates the use of E-PL as natural antimicrobial to inhibit fungal growth and to reduce aflatoxins&lt;br&gt; (AFs) production. Antifungal activity of starch biofilms with different concentrations of E-Poly-&lt;br&gt; L-lysine (E-PL) was determined in solid medium against Aspergillus parasiticus (AFs producer) and&lt;br&gt; Penicillium expansum. Then, biofilms were tested as antimicrobial devices for the preservation of&lt;br&gt; bread loaf inoculated with A. parasiticus CECT 2681 and P. expansum CECT 2278. Shelf life and&lt;br&gt; AFs content were examined. Biofilms with concentrations of E-PL less than 1.6 mg/cm2 showed&lt;br&gt; no fungal growth inhibition in solid medium, while the antifungal activity of the films with greater&lt;br&gt; than 1.6 mg/cm2 of E-PL was dose dependent. The shelf life of bread inoculated with A. parasiticus&lt;br&gt; was increased by 1 day with the use of films containing 1.6–6.5 mg E-PL/cm2, while shelf life of&lt;br&gt; bread tainted with P. expansum was increased by 3 day with 6.5 mg E-PL/cm2. AFs production&lt;br&gt; was greatly inhibited by E-PL biofilms (93–100%). Thus, E-PL biofilms could be potentially used as&lt;br&gt; antimicrobial device during bread storage as a natural alternative to the synthetic preservatives.&lt;/p&gt;</dct:description>
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    <dct:title>Integrated and innovative key actions for mycotoxin management in the food and feed chain</dct:title>
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