2024-03-28T21:07:18Z
https://zenodo.org/oai2d
oai:zenodo.org:3258663
2019-09-24T17:05:39Z
user-openlabnotebooks
user-truantlab
user-labscribbles
Maiuri, Tamara
2019-06-27
<p>Year-end report for the HDSA Berman-Topper fellowship funding the ROS-specific huntingtin interactor project. End of Year 2: 2019-07-01. </p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.3258663
oai:zenodo.org:3258663
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.3258662
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Huntington's disease
huntingtin
ROS
reactive oxygen species
DNA repair
poly ADP ribose
PARP
HDSA
Berman Topper
2019-07-01 Berman-Topper Year-end Progress Report
info:eu-repo/semantics/other
oai:zenodo.org:1411460
2020-01-24T19:25:24Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2018-09-07
<p>Poly-ADP ribose overlay assay with purified huntingtin protein to test ability of full-length and fragment huntingtin to bind poly-ADP ribose.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.1411460
oai:zenodo.org:1411460
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.1411459
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
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huntingtin
Huntington's disease
reactive oxygen species
ROS
poly-ADP ribose
DNA repair
oxidative stress
ROS-specific Huntingtin Interactions: In vitro poly-ADP ribose binding assays
info:eu-repo/semantics/other
oai:zenodo.org:896813
2020-01-24T19:25:44Z
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2017-09-19
<p>Optimization step in the lead up to phenotypic analysis of ROS-dependent huntingtin interacting proteins.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship
https://doi.org/10.5281/zenodo.896813
oai:zenodo.org:896813
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.896812
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
hun
Huntington's disease
ROS
rea
GFP reactivation
assay
ROS-specific Huntingtin Interactions: GFP reactivation assay WT vs HD mouse striatal cells second attempt
info:eu-repo/semantics/other
oai:zenodo.org:1039934
2020-01-24T19:25:58Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2017-10-31
<p>Optimization step in the development of an inducible system expressing YFP-tagged huntingtin-specific intrabodies for stable transfection in TruHD fibroblasts.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship
https://doi.org/10.5281/zenodo.1039934
oai:zenodo.org:1039934
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.1039933
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
intrabody
chromobody
inducible system
stable transfection
ROS-specific Huntingtin Interactions: Inducible huntingtin-specific chromobody expression by nucleofection
info:eu-repo/semantics/other
oai:zenodo.org:1319540
2020-01-24T19:25:16Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2018-07-23
<p>Mass spec identification of DNA damage-specific huntingtin protein-protein interactions.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.1319540
oai:zenodo.org:1319540
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.1319539
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Huntington's disease
huntingtin
protein-protein interactions
mass spectrometry
ROS
reactive oxygen species
DNA damage
DNA repair
ROS-Specific Huntingtin Interactions: Reproducible high and medium confidence huntingtin interacting proteins
info:eu-repo/semantics/other
oai:zenodo.org:3552757
2020-01-24T19:25:55Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2019-11-25
<p>Attempts to use the PBM3 peptide in a competition assay to measure the on-off rates of huntingtin PAR binding.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.3552757
oai:zenodo.org:3552757
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.3552756
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
oxidative stress
poly ADP ribose
PARP
PAR overlay assay
MABE1016
ROS-Specific Huntingtin Interactions: Competition of huntingtin PAR binding by PBM3 peptide
info:eu-repo/semantics/other
oai:zenodo.org:3251029
2020-01-24T19:25:18Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2019-06-20
<p>Optimization of an assay to measure huntingtin chromatin retention in response to oxidative stress, using the YFP-tagged huntingtin-specific intrabody nucHCB2.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.3251029
oai:zenodo.org:3251029
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.3251028
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Huntingtin
Huntington's disease
ROS
reactive oxygen species
chromatin retention assay
oxidative stress
DNA repair
intrabody
RPE1
ROS-Specific Huntingtin Interactions: Chromatin Retention Assay Set-up
info:eu-repo/semantics/other
oai:zenodo.org:1162264
2020-01-24T19:25:28Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2018-01-29
<p>Mass spec identification of ROS-specific huntingtin protein-protein interactions. </p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.1162264
oai:zenodo.org:1162264
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.1162263
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
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huntingtin
Huntington's disease
ROS
reactive oxygen species
protein-protein interactions
hydrogen peroxide
H2O2
mouse striatal cells
STHdh
ROS-Specific Huntingtin Interactions: Mass spec analysis of ROS-treated mouse striatal cells
info:eu-repo/semantics/other
oai:zenodo.org:3552780
2020-01-24T19:25:10Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Harding, Rachel
Truant, Ray
2019-11-25
<p>Attempts to test PAR binding of huntingtin purified with nickel or heparin.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.3552780
oai:zenodo.org:3552780
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.3552779
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
oxidative stress
poly ADP ribose
PARP
PAR overlay assay
MABE1016
ROS-Specific Huntingtin Interactions: Testing PAR binding of huntingtin purified with nickel or heparin
info:eu-repo/semantics/other
oai:zenodo.org:833498
2020-01-24T19:25:17Z
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2017-07-21
<p>Optimization step in the lead up to mass spec identification of ROS-specific huntingtin protein-protein interactions.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.833498
oai:zenodo.org:833498
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.833497
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
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huntingtin
huntington's disease
fractionation
reactive oxygen species
protein-protein interactions
ROS
ROS-specific Huntingtin Interactions: Fractionation Optimization
info:eu-repo/semantics/other
oai:zenodo.org:2617679
2020-01-20T12:04:04Z
user-truantlab
openaire
Maiuri, Tamara
Barba, Carlos
Harding, Rachel
Truant, Ray
2019-03-31
<p><strong>Abstract</strong></p>
<p> Huntington’s disease (HD) age of onset is correlated to CAG repeat length within the disease-causing gene but varies by decades between individuals. Genetic causes for this disease modification were linked to DNA repair, redox regulation, and mitochondrial health pathways by genome-wide association studies. The huntingtin protein responds to reactive oxygen species by moving to the nucleus where it localizes to sites of DNA damage and acts as a scaffold for DNA repair factors. We therefore sought to identify huntingtin-interacting proteins in the context of oxidative stress. We found a large degree of overlap with databases of proteins modified by poly ADP ribose (PAR), the moiety generated upon activation of poly ADP ribose polymerases (PARPs) by DNA damage. We show that purified huntingtin protein binds PAR in vitro and that huntingtin co-immunoprecipitates PARylated proteins upon oxidative stress.</p>
<p> We and others have previously observed elevated levels of DNA damage in HD cells and tissues, which may reflect suboptimal huntingtin function as a DNA repair factor scaffold. Unrepaired DNA damage leads to prolonged activation of PARPs and overproduction of PAR. We therefore examined PAR levels in HD patient-derived fibroblasts and found them to be elevated compared to controls. Unrelenting PAR production causes ATP depletion, mitochondrial failure and energy crisis through multiple mechanisms, providing a link between GWAS genetic modifier pathways and long-observed energy deficits in HD. PAR also acts as a mediator of cell death through parthanatos. Current efforts are therefore focused on determining whether inhibition of PAR production can alleviate ATP/ADP ratio phenotypes and cell death in HD cells. We seek to determine if PARP enzymatic inhibition, or suppression of PARP expression is the target for HD. Recent reports of the role of PAR in Parkinson’s and other neurodegenerative diseases suggests a common pathogenic mechanism and that repurposing PARP inhibitors, some of which have been developed and FDA-approved as cancer drugs, may provide a viable therapeutic strategy.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship. This poster was presented at the 2019 HD Therapeutics conference in Palm Springs, CA, Feb 25-28.
https://doi.org/10.5281/zenodo.2617679
oai:zenodo.org:2617679
eng
Zenodo
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.2617678
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
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Huntingtin
Huntington's disease
Poly ADP ribose
ROS
Reactive oxygen species
Veliparib
PARP
DNA Repair
oxidative stress
protein-protein interactions
Dysregulation of poly ADP ribose signaling in HD and its potential as a therapeutic target
info:eu-repo/semantics/conferencePoster
oai:zenodo.org:3258526
2019-07-03T19:26:08Z
user-openlabnotebooks
user-truantlab
user-labscribbles
Maiuri, Tamara
2019-06-27
<p>Quarterly and year-end reports for the HDSA Berman-Topper fellowship funding the ROS-specific huntingtin interactor project. Reports dated 2017-10-01, 2018-01-01, 2018-04-01, 2018-07-01 (year-end), 2018-10-01.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.3258526
oai:zenodo.org:3258526
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.3258525
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Huntington's disease
huntingtin
ROS
reactive oxygen species
DNA repair
poly ADP ribose
PARP
HDSA
Berman Topper
2017-2018 Berman-Topper Progress Reports
info:eu-repo/semantics/other
oai:zenodo.org:3715132
2022-09-29T17:10:55Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2020-03-18
<p>Comparison nuclear PAR levels in wild type and HD (TruHD) fibroblasts in response to a PARG inhibitor concentration gradient (as a measure of PARG activity).</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.3715132
oai:zenodo.org:3715132
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.3715131
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
oxidative stress
poly ADP ribose
PARG
TruHD cells
MABE1016
ROS-Specific Huntingtin Interactions: Testing PARG activity in HD patient fibroblasts round 2
info:eu-repo/semantics/other
oai:zenodo.org:1464979
2020-01-24T19:26:25Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Barba Bazan, Carlos
Maiuri, Tamara
Truant, Ray
2018-10-17
<p>Comparison of poly ADP ribose levels in HD cells versus controls in response to ROS.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.1464979
oai:zenodo.org:1464979
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.1464978
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
reactive oxygen species
ROS
poly ADP ribose
DNA repair
oxidative stress
MABE1016
ROS-Specific Huntingtin Interactions: Measuring poly ADP ribose levels in HD cells
info:eu-repo/semantics/other
oai:zenodo.org:3552720
2020-01-24T19:26:05Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2019-11-25
<p>Optimization to find conditions allowing quantification of huntingtin PAR binding in the PAR overlay assay.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.3552720
oai:zenodo.org:3552720
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.3552719
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
oxidative stress
poly ADP ribose
PARP
PAR overlay assay
MABE1016
DNA repair
ROS-Specific Huntingtin Interactions: PAR overlay slot blotting optimization
info:eu-repo/semantics/other
oai:zenodo.org:3251038
2020-01-24T19:25:43Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2019-06-20
<p>Huntingtin chromatin retention in response to oxidative stress in wild type, PARP1 knockout, PARP2 knockout, and PARP1/PARP2 knockout RPE1 cells.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.3251038
oai:zenodo.org:3251038
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.3251037
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
oxidative stress
chromatin retention assay
PARP
poly ADP ribose
intrabody
ROS-Specific Huntingtin Interactions: Chromatin Retention of Huntingtin in PARP KO Cells
info:eu-repo/semantics/other
oai:zenodo.org:3560227
2020-01-24T19:26:12Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2019-12-02
<p>Comparison nuclear PAR levels in wild type and HD (TruHD) fibroblasts in response to a PARG inhibitor concentration gradient (as a measure of PARG activity).</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.3560227
oai:zenodo.org:3560227
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.3560226
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
oxidative stress
poly ADP ribose
PARG
ROS-Specific Huntingtin Interactions: Testing PARG activity in HD patient fibroblasts
info:eu-repo/semantics/other
oai:zenodo.org:4536373
2022-09-29T17:10:54Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2021-02-11
<p>Immunofluorescence of N17-phosphorylated huntingtin in PARG inhibitor-treated cells reveals a "chromosome coating" effect.</p>
https://doi.org/10.5281/zenodo.4536373
oai:zenodo.org:4536373
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.4536372
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
poly ADP ribose
PARP
PARG
Mitosis
Immunofluorescence
PAR dysregulation in Huntington's disease: huntingtin immunofluorescence in PARG inhibitor-treated cells
info:eu-repo/semantics/other
oai:zenodo.org:833500
2020-01-24T19:25:25Z
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2017-07-21
<p>Optimization step in the lead up to mass spec identification of ROS-specific huntingtin protein-protein interactions.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.833500
oai:zenodo.org:833500
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.833499
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
reactive oxygen species
ROS
protein-protein interactions
ROS-specific Huntingtin Interactions: Crosslinking Optimization
info:eu-repo/semantics/other
oai:zenodo.org:3251044
2020-01-24T19:25:09Z
user-sgc-opennotebook
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2019-06-20
<p>Optimization of conditions for the detection of poly ADP ribose by immunofluorescence.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.3251044
oai:zenodo.org:3251044
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/sgc-opennotebook
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.3251043
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
oxidative stress
poly ADP ribose
PARP
RPE1
MABE1016
ROS-Specific Huntingtin Interactions: Optimization of Poly ADP Ribose Detection by Immunofluorescence
info:eu-repo/semantics/other
oai:zenodo.org:896809
2020-01-24T19:24:07Z
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2017-09-19
<p>Optimization step in the lead up to phenotypic analysis of ROS-dependent huntingtin interacting proteins.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fel
https://doi.org/10.5281/zenodo.896809
oai:zenodo.org:896809
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.896808
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
DNA repair
GFP reactivation
assay
ROS-specific Huntingtin Interactions: GFP reactivation assay WT vs HD mouse striatal cells
info:eu-repo/semantics/other
oai:zenodo.org:896797
2020-01-24T19:25:18Z
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2017-09-19
<p>Optimization step in the lead up to phenotypic analysis of ROS-dependent huntingtin interacting proteins.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.896797
oai:zenodo.org:896797
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.896796
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
DNA repair
GFP reactivation
assay
ROS-specific Huntingtin Interactions: GFP reactivation assay optimization in HEK 293 cells
info:eu-repo/semantics/other
oai:zenodo.org:3560232
2020-01-24T19:26:15Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2019-12-02
<p>Comparison of nuclear PAR levels in wild type and HD (TruHD) fibroblasts in response to a PARP inhibitor concentration gradient (as a measure of PARP activity).</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.3560232
oai:zenodo.org:3560232
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.3560231
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
oxidative stress
poly ADP ribose
PARP
ROS-Specific Huntingtin Interactions: Testing PARP activity in HD patient fibroblasts
info:eu-repo/semantics/other
oai:zenodo.org:2586924
2020-01-24T19:26:19Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2019-03-07
<p>Huntingtin co-immunoprecipitation of proteins modified by poly ADP ribose.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.2586924
oai:zenodo.org:2586924
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.2586923
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Huntingtin
Huntington's disease
Poly ADP ribose
ROS
Reactive oxygen species
Co-immunoprecipitation
PARP
Veliparib
DNA repair
oxidative stress
ROS-Specific Huntingtin Interactions: Huntingtin pulldown of PARylated proteins
info:eu-repo/semantics/other
oai:zenodo.org:839262
2020-01-24T19:26:18Z
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2017-08-05
<p>Optimization step in the lead up to mass spec identification of ROS-specific huntingtin protein-protein interactions.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.839262
oai:zenodo.org:839262
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.839261
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
reactive oxygen species
ROS
protein-protein interactions
3-nitropropionic acid
ROS-specific Huntingtin Interactions: Oxidative Stress Optimization 3NP
info:eu-repo/semantics/other
oai:zenodo.org:1218379
2020-01-24T19:24:51Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2018-04-14
<p>Mass spec identification of DNA damage-specific huntingtin protein-protein interactions. </p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.1218379
oai:zenodo.org:1218379
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.1218378
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Huntington's disease
huntingtin
protein-protein interactions
mass spectrometry
ROS
reactive oxygen species
DNA damage
DNA repair
mouse striatal cells
STHdh
ROS-Specific Huntingtin Interactions: Mass spec analysis of MMS-treated mouse striatal cells
info:eu-repo/semantics/other
oai:zenodo.org:839256
2020-01-24T19:24:52Z
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2017-08-05
<p>Optimization step in the lead up to mass spec identification of ROS-specific huntingtin protein-protein interactions.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.839256
oai:zenodo.org:839256
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.839255
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
reactive oxygen species
ROS
protein-protein interactions
ROS-specific Huntingtin Interactions: Oxidative Stress Optimization H2O2
info:eu-repo/semantics/other
oai:zenodo.org:1001761
2020-01-24T19:23:20Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2017-10-03
<p>Optimization step in the development of an inducible system expressing YFP-tagged huntingtin-specific intrabodies for stable transfection in TruHD fibroblasts.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship
https://doi.org/10.5281/zenodo.1001761
oai:zenodo.org:1001761
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.1001760
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
stable transfection
inducible system
intrabody
chromobody
ROS-specific Huntingtin Interactions: G418 kill curve in TruHD fibroblasts
info:eu-repo/semantics/other
oai:zenodo.org:1133700
2020-01-24T19:25:35Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2017-12-28
<p>Optimization step in the lead up to phenotypic analysis of ROS-dependent huntingtin interacting proteins. </p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.1133700
oai:zenodo.org:1133700
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.1133699
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
GFP reactivation assay
ROS-specific Huntingtin Interactions: GFP reactivation assay in HD patient fibroblasts
info:eu-repo/semantics/other
oai:zenodo.org:3459660
2020-01-24T19:25:10Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2019-09-24
<p>Huntingtin amino acids 1790-1798 make up a potential PAR binding motif (PBM3). Two huntingtin fragments (1208-1810 and 1775-2413) were tested for chromatin retention upon oxidative stress.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.3459660
oai:zenodo.org:3459660
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.3459659
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
oxidative stress
poly ADP ribose
chromatin retention assay
ROS-Specific Huntingtin Interactions: Chromatin retention assay with huntingtin fragments containing PBM3
info:eu-repo/semantics/other
oai:zenodo.org:2539483
2020-01-24T19:26:21Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2019-01-14
<p>In vitro testing of peptides representing potential poly ADP ribose binding motifs within the huntingtin sequence.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.2539483
oai:zenodo.org:2539483
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.2539482
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Huntingtin
Huntington's disease
Poly ADP ribose
ROS
Reactive oxygen species
PAR overlay assay
DNA repair
oxidative stress
MABE1016
ROS-Specific Huntingtin Interactions: Peptide poly ADP ribose overlay assay
info:eu-repo/semantics/other
oai:zenodo.org:846319
2020-01-24T19:25:20Z
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2017-08-21
<p>Optimization step in the lead up to mass spec identification of ROS-specific huntingtin protein-protein interactions.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.846319
oai:zenodo.org:846319
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.846318
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
oxidative stress
protein-protein interactions
reactive oxygen species
immunoprecipitation
ROS-Specific Huntingtin Interactions: IP Optimization in Patient-Derived Cells
info:eu-repo/semantics/other
oai:zenodo.org:1407221
2020-01-21T07:22:30Z
user-sgc-opennotebook
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Harding, Rachel
Maiuri, Tamara
Truant, Ray
Arrowsmith, Cheryl
Edwards, Aled
2018-08-31
<p><strong>Project </strong>- Huntingtin structure-function open lab notebook. </p>
<p><strong>Rationale</strong> - To compare the ROS-specific huntingtin interaction partners identified by Dr. Tamara Maiuri with those detailed in existing databases. </p>
<p><strong>Overview</strong> - Previously, different online databases which detail protein interaction partners were searched for huntingtin protein interaction partners. Following completion of this initial analysis, Dr. Tamara Maiuri posted in her open notebook a detailed list of high and medium confidence ROS-specific huntingtin interacting proteins: <a href="https://zenodo.org/record/1319540">https://zenodo.org/record/1319540</a>. A comparison of these interactors with those identified in the previously mined databases is briefly detailed. </p>
Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research. This project is funded and supported with generous funding from the Huntington Society of Canada and the CHDI Foundation. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.
https://doi.org/10.5281/zenodo.1407221
oai:zenodo.org:1407221
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/sgc-opennotebook
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.1407220
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Huntingtin
HTT
Huntington's disease
HD
Interaction
Proten-protein interaction
PPI
Comparative analysis of huntingtin interaction partner database searches with Dr. Maiuri's work - 2018/08/31
info:eu-repo/semantics/other
oai:zenodo.org:2587029
2020-01-24T19:25:17Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2019-03-07
<p>Testing chromatin retention of huntingtin fragment 1-586 upon PARP inhibition.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.2587029
oai:zenodo.org:2587029
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.2587028
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Huntingtin
Huntington's disease
Chromatin retention assay
Poly ADP ribose
ROS
Reactive oxygen species
Veliparib
PARP
DNA Repair
oxidative stress
ROS-Specific Huntingtin Interactions: huntingtin 1-586 chromatin retention assay with veliparib
info:eu-repo/semantics/other
oai:zenodo.org:3251981
2020-01-21T07:23:13Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2019-06-21
<p>Comparison of poly ADP ribose levels in Q7/Q7 versus Q111/Q111 STHdh cells in response to oxidative stress.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.3251981
oai:zenodo.org:3251981
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.3251980
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
reactive oxygen species
ROS
oxidative stress
poly ADP ribose
PARP
STHdh
MABE1016
DNA damage
DNA repair
ROS-Specific Huntingtin Interactions: Poly ADP Ribose Levels in STHdh Cells
info:eu-repo/semantics/other
oai:zenodo.org:3338633
2020-01-24T19:26:07Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2019-07-16
<p>Measurement of huntingtin chromatin recruitment dynamics by fluorescence recovery after photobleaching (FRAP) of the YFP-tagged huntingtin-specific intrabody, nucHCB2, under conditions of oxidative stress and PARP inhibition</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.3338633
oai:zenodo.org:3338633
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.3338632
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
oxidative stress
poly ADP ribose
PARP
RPE1
DNA repair
intrabody
FRAP
veliparib
ROS-Specific Huntingtin Interactions: Huntingtin chromatin retention dynamics by FRAP with veliparib
info:eu-repo/semantics/other
oai:zenodo.org:1039926
2020-01-24T19:23:48Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2017-10-31
<p>Optimization step in the development of an inducible system expressing YFP-tagged huntingtin-specific intrabodies for stable transfection in TruHD fibroblasts.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship
https://doi.org/10.5281/zenodo.1039926
oai:zenodo.org:1039926
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.1039925
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
intrabody
chromobody
inducible system
stable transfection
ROS-specific Huntingtin Interactions: Comparing transfection methods for inducible expression of huntingtin-specific chromobody
info:eu-repo/semantics/other
oai:zenodo.org:3458208
2020-01-24T19:26:15Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2019-09-23
<p>Poly-ADP ribose overlay assay with huntingtin PBM peptides to test ability of a PBM3 alanine mutant to bind poly-ADP ribose.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.3458208
oai:zenodo.org:3458208
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.3458207
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
poly ADP ribose
DNA repair
MABE1016
ROS-Specific Huntingtin Interactions: PAR overlay assay with PBM3 peptide mutant
info:eu-repo/semantics/other
oai:zenodo.org:1123459
2020-01-21T07:23:10Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2017-12-21
<p>Optimization step in the lead up to mass spec identification of ROS-specific huntingtin protein-protein interactions. </p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.1123459
oai:zenodo.org:1123459
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.1123458
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
protein-protein interactions
3-nitropropionic acid
potassium bromate
hydrogen peroxide
3-NP
KBrO3
H2O2
STHdh
mouse striatal cells
ROS-Specific Huntingtin Interactions: ROS Source Optimization in Mouse Striatal Cells
info:eu-repo/semantics/other
oai:zenodo.org:3459522
2020-01-24T19:25:36Z
user-openlabnotebooks
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2019-09-24
<p>PAR overlay assay with different preparations of huntingtin has yielded variable results. These results are summarized.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.3459522
oai:zenodo.org:3459522
eng
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/openlabnotebooks
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.3459521
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
poly ADP ribose
PAR overlay assay
ROS-Specific Huntingtin Interactions: Summary of PAR binding for different huntingtin protein preps
info:eu-repo/semantics/other
oai:zenodo.org:1001041
2020-01-24T19:26:13Z
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2017-10-02
<p>Testing step in the generation of an inducible system for expressing YFP-tagged huntingtin-specific intrabodies in live cells.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship.
https://doi.org/10.5281/zenodo.1001041
oai:zenodo.org:1001041
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.1001040
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
intrabody
chromobody
inducible system
ROS-specific Huntingtin Interactions: Testing inducible expression of huntingtin-specific chromobodies
info:eu-repo/semantics/other
oai:zenodo.org:1001030
2020-01-24T19:24:36Z
user-truantlab
user-labscribbles
openaire_data
Maiuri, Tamara
Truant, Ray
2017-10-02
<p>Cloning step in the generation of an inducible system for expressing YFP-tagged huntingtin-specific intrabodies in live cells.</p>
This work is funded by the HDSA Berman/Topper HD Career Development Fellowship
https://doi.org/10.5281/zenodo.1001030
oai:zenodo.org:1001030
Zenodo
https://zenodo.org/communities/labscribbles
https://zenodo.org/communities/truantlab
https://doi.org/10.5281/zenodo.1001029
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
huntingtin
Huntington's disease
ROS
reactive oxygen species
intrabody
chromobody
inducible system
ROS-specific Huntingtin Interactions: Cloning huntingtin-specific chromobodies into pTRE-3G
info:eu-repo/semantics/other