Published January 10, 2024 | Version v1
Dataset Open

Silva v132 eukaryotic 18S non-redundant database formatted for DADA2

  • 1. ROR icon Forest Science and Technology Centre of Catalonia

Description

This file is derived from the Silva v132 eukaryotic SSU database. This database has been curated correcting and completing the taxonomy, removing redundant sequences and leaving a single sequence per species (the longest).

The database consists of 26367 sequences. 29337 sequences from eukaryotic organisms, 20 from bacteria and 10 from archaea. Sequences are identified down to species level. This file is designed specifically for use in the classification of eukaryotic 18S sequencing data.

If you use this database in your work, please cite the reference to the Silva database (Quast et al., 2013), the article from which this document is derived (del Portillo et al., 2024) and this Zenodo repository.

 

 

The SILVA v132 database is release under a Creative Commons Attribution 4.0 (CC-BY 4.0) license. Thus documents are freely available for academic and commercial use as long as SILVA is credited as original author and a link to the full license is provided.

https://creativecommons.org/licenses/by/4.0/

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Additional details

References

  • Callahan, B. J., McMurdie, P. J., Rosen, M. J., Han, A. W., Johnson, A. J. A., & Holmes, S. P. (2016). DADA2: High-resolution sample inference from Illumina amplicon data. Nature methods, 13(7), 581-583. https://doi.org/10.1038/nmeth.3869
  • del Portillo, D. G., Cabodevilla, X., Arroyo, B., & Morales, M. B. (2024). Diet preferences of a declining steppe bird during the critical chick rearing period as revealed by DNA metabarcoding. Under Review
  • Quast, C., Pruesse, E., Yilmaz, P., Gerken, J., Schweer, T., Yarza, P., ... & Glöckner, F. O. (2012). The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic acids research, 41(D1), D590-D596. https://doi.org/10.1093/nar/gks1219