	galM	glk	pgm	pgi	pfkA_2	pfkB	fbp	fbaB	gapC	gpmA	gpmB	pykA	pykF	ppsA	zwf	pgl	gnd	rpe	rpiA	rpiB	tktA	tktB	talA	talB	"WT, 0.1h-1"	"WT, 0.4h-1"	"WT, 0.5h-1"	"WT, 0.7h-1"
Glucose + PEP -> G6P + PYR	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 	100 
G6P <-> F6P	64 	87 	78 	0 	84 	52 	85 	73 	63 	83 	88 	74 	47 	68 	99 	78 	99 	92 	67 	51 	78 	86 	85 	86 	62 	63 	83 	56 
"F6P -> F1,6P"	81 	89 	86 	34 	87 	77 	87 	83 	80 	86 	90 	84 	75 	82 	93 	86 	93 	92 	69 	79 	79 	88 	87 	88 	80 	79 	87 	80 
"F1,6P -> DHAP + G3P"	81 	89 	86 	34 	87 	77 	87 	83 	80 	86 	90 	84 	75 	82 	93 	86 	93 	92 	69 	79 	79 	88 	87 	88 	80 	79 	87 	80 
DHAP -> G3P	81 	89 	86 	34 	87 	77 	87 	83 	80 	86 	90 	84 	75 	82 	93 	86 	93 	92 	69 	79 	79 	88 	87 	88 	80 	79 	87 	80 
G3P -> 3PG	168 	177 	173 	120 	173 	163 	173 	169 	168 	172 	178 	170 	161 	170 	181 	173 	182 	181 	157 	169 	171 	175 	174 	175 	167 	165 	173 	170 
3PG <-> PEP	159 	167 	164 	109 	162 	153 	162 	159 	158 	161 	169 	160 	150 	160 	171 	162 	173 	173 	148 	162 	164 	165 	163 	165 	157 	153 	162 	162 
PEP -> PYR	55 	51 	50 	5 	43 	49 	57 	45 	54 	41 	55 	51 	46 	35 	68 	51 	67 	70 	45 	52 	48 	40 	39 	41 	53 	25 	34 	43 
PYR -> AcCoA + CO2	141 	132 	130 	122 	120 	130 	142 	123 	144 	118 	137 	129 	132 	114 	156 	131 	149 	153 	145 	138 	147 	120 	117 	121 	136 	101 	112 	127 
G6P -> 6PG	35 	12 	21 	98 	14 	47 	14 	25 	36 	16 	11 	24 	52 	31 	0 	20 	0 	7 	31 	48 	21 	13 	13 	13 	36 	36 	15 	42 
6PG -> Ru5P + CO2	35 	12 	21 	61 	14 	47 	14 	25 	36 	16 	11 	24 	52 	31 	0 	20 	0 	7 	12 	48 	8 	13 	13 	13 	36 	36 	15 	42 
Ru5P -> X5P	18 	3 	8 	35 	3 	25 	3 	11 	19 	5 	3 	10 	29 	15 	-5 	8 	-5 	0 	3 	28 	2 	3 	3 	3 	19 	18 	4 	24 
Ru5P -> R5P	17 	9 	12 	26 	11 	21 	11 	14 	17 	11 	9 	14 	23 	16 	5 	12 	5 	7 	9 	20 	6 	10 	10 	10 	18 	18 	11 	18 
R5P + X5P <-> S7P + G3P	10 	3 	6 	19 	3 	14 	3 	7 	11 	4 	3 	7 	16 	9 	-1 	6 	-1 	1 	3 	15 	2 	3 	3 	3 	11 	11 	4 	13 
S7P + G3P <-> E4P + F6P	10 	3 	6 	19 	3 	14 	3 	7 	11 	4 	3 	7 	16 	9 	-1 	6 	-1 	1 	3 	15 	2 	3 	3 	3 	11 	11 	4 	13 
X5P + E4P <-> F6P + G3P	7 	0 	3 	16 	0 	11 	0 	4 	8 	1 	0 	4 	13 	6 	-4 	3 	-4 	-1 	0 	13 	0 	0 	0 	0 	8 	7 	0 	11 
AcCoA + OAA -> CIT	87 	98 	93 	67 	82 	76 	82 	80 	85 	80 	104 	83 	73 	87 	103 	88 	108 	111 	99 	110 	129 	92 	86 	91 	82 	70 	72 	89 
CIT -> ICT	87 	98 	93 	67 	82 	76 	82 	80 	85 	80 	104 	83 	73 	87 	103 	88 	108 	111 	99 	110 	129 	92 	86 	91 	82 	70 	72 	89 
ICT -> 2-KG + CO2	61 	91 	82 	41 	73 	51 	52 	67 	54 	73 	96 	65 	43 	87 	76 	73 	93 	94 	79 	101 	129 	92 	83 	89 	56 	70 	72 	89 
2-KG -> SUC + CO2	53 	83 	74 	33 	64 	43 	43 	58 	46 	64 	89 	57 	34 	79 	68 	65 	85 	87 	72 	95 	123 	84 	75 	81 	48 	60 	63 	83 
SUC -> FUM	79 	91 	85 	58 	73 	68 	73 	71 	77 	72 	97 	74 	64 	79 	95 	80 	101 	105 	92 	104 	123 	84 	77 	83 	74 	60 	63 	83 
FUM -> MAL	79 	91 	85 	58 	73 	68 	73 	71 	77 	72 	97 	74 	64 	79 	95 	80 	101 	105 	92 	104 	123 	84 	77 	83 	74 	60 	63 	83 
MAL <-> OAA	100 	98 	96 	81 	81 	90 	95 	85 	98 	79 	105 	92 	87 	79 	115 	94 	117 	122 	111 	112 	123 	84 	79 	85 	96 	60 	63 	83 
PEP + CO2 <-> OAA	0 	13 	10 	0 	15 	0 	1 	10 	0 	16 	11 	5 	0 	21 	0 	7 	3 	0 	0 	7 	14 	21 	20 	20 	0 	25 	24 	17 
MAL -> PYR + CO2	5 	0 	0 	2 	0 	3 	8 	0 	10 	0 	0 	0 	7 	0 	7 	0 	0 	0 	0 	0 	0 	0 	0 	0 	4 	0 	0 	0 
ICT -> Glyoxylate + SUC	26 	7 	11 	25 	8 	25 	30 	13 	31 	7 	8 	18 	30 	0 	27 	15 	16 	17 	20 	8 	0 	0 	2 	2 	26 	0 	0 	0 
Glyoxylate + AcCoA -> MAL	26 	7 	11 	25 	8 	25 	30 	13 	31 	7 	8 	18 	30 	0 	27 	15 	16 	17 	20 	8 	0 	0 	2 	2 	26 	0 	0 	0 
AcCoA -> Acetate	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	7 	14 
PYR -> Lactate	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 
AcCoA -> Ethanol	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	0 	2 	0 	0 	0 	0 	0 	0 	0 	0 	2 	1 
G6P -> (Cell synthesis)	1 	1 	1 	2 	2 	2 	2 	2 	2 	2 	1 	2 	2 	2 	1 	2 	1 	1 	1 	1 	1 	2 	2 	2 	2 	2 	2 	1 
F6P -> (Cell synthesis)	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 
R5P -> (Cell synthesis)	7 	6 	6 	7 	7 	7 	7 	7 	7 	7 	6 	7 	7 	7 	6 	7 	6 	5 	6 	5 	4 	7 	7 	7 	7 	8 	7 	5 
E4P -> (Cell synthesis)	3 	3 	3 	3 	3 	3 	3 	3 	3 	3 	3 	3 	3 	3 	3 	3 	3 	2 	3 	2 	2 	3 	3 	3 	3 	3 	3 	2 
G3P -> (Cell synthesis)	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 	1 
3PG -> (Cell synthesis)	10 	9 	10 	11 	11 	11 	11 	11 	10 	11 	9 	11 	11 	10 	9 	10 	9 	8 	9 	7 	7 	10 	11 	10 	10 	11 	11 	8 
PEP -> (Cell synthesis)	4 	3 	4 	4 	4 	4 	4 	4 	4 	4 	3 	4 	4 	4 	3 	4 	3 	3 	3 	3 	2 	4 	4 	4 	4 	4 	4 	3 
PYR -> (Cell synthesis)	20 	19 	20 	22 	23 	22 	23 	22 	20 	23 	18 	22 	22 	20 	19 	21 	18 	17 	19 	15 	14 	21 	22 	21 	21 	24 	23 	16 
AcCoA -> (Cell synthesis)	27 	26 	27 	30 	30 	29 	30 	30 	27 	30 	25 	29 	29 	27 	26 	28 	24 	23 	26 	20 	18 	28 	29 	28 	28 	31 	30 	21 
OAA -> (Cell synthesis)	13 	13 	13 	15 	15 	14 	15 	14 	13 	15 	12 	14 	14 	13 	12 	14 	12 	11 	12 	10 	9 	13 	14 	14 	14 	15 	15 	10 
2KG -> (Cell synthesis)	8 	8 	8 	9 	9 	9 	9 	9 	8 	9 	7 	9 	9 	8 	8 	8 	7 	7 	8 	6 	5 	8 	9 	8 	8 	9 	9 	6 
CO2 -> (Evolution)	295 	305 	296 	259 	256 	273 	257 	263 	289 	255 	322 	270 	268 	290 	307 	282 	324 	341 	307 	376 	393 	287 	268 	283 	281 	243 	238 	325 
Exch. (G6P <-> F6P)	0.91 	0.95 	0.93 	0.00 	0.94 	0.83 	0.74 	0.95 	0.90 	0.94 	0.82 	0.89 	0.87 	0.92 	0.10 	0.92 	0.00 	0.00 	0.33 	0.94 	0.87 	0.94 	0.57 	0.38 	0.95 	0.95 	0.93 	0.95 
Exch. (3PG <-> PEP)	0.00 	0.95 	0.95 	0.42 	0.95 	0.00 	0.94 	0.00 	0.00 	0.95 	0.95 	0.84 	0.01 	0.92 	0.91 	0.93 	0.95 	0.95 	0.95 	0.95 	0.95 	0.95 	0.95 	0.95 	0.95 	0.95 	0.00 	0.95 
Exch. (R5P + X5P <-> S7P + G3P)	0.00 	0.00 	0.00 	0.95 	0.00 	0.00 	0.00 	0.00 	0.00 	0.01 	0.00 	0.00 	0.00 	0.00 	0.02 	0.00 	0.56 	0.71 	0.45 	0.94 	0.00 	0.00 	0.00 	0.00 	0.01 	0.09 	0.07 	0.00 
Exch. (S7P + G3P <-> E4P + F6P)	0.70 	0.59 	0.68 	0.53 	0.48 	0.71 	0.66 	0.58 	0.53 	0.59 	0.56 	0.67 	0.67 	0.60 	0.47 	0.55 	0.06 	0.35 	0.65 	0.57 	0.42 	0.63 	0.62 	0.45 	0.65 	0.52 	0.54 	0.34 
Exch. (X5P + E4P <-> F6P + G3P)	0.01 	0.00 	0.00 	0.12 	0.00 	0.00 	0.00 	0.00 	0.00 	0.01 	0.00 	0.00 	0.00 	0.00 	0.00 	0.00 	0.11 	0.08 	0.17 	0.16 	0.00 	0.00 	0.02 	0.02 	0.06 	0.12 	0.01 	0.00 
Exch. (MAL <-> OAA)	0.93 	0.94 	0.95 	0.51 	0.95 	0.85 	0.93 	0.91 	0.86 	0.90 	0.94 	0.93 	0.93 	0.92 	0.93 	0.93 	0.81 	0.89 	0.92 	0.94 	0.84 	0.94 	0.93 	0.93 	0.94 	0.95 	0.87 	0.94 
Exch. (PEP + CO2 <-> OAA)	0.30 	0.31 	0.35 	0.08 	0.45 	0.28 	0.40 	0.29 	0.25 	0.31 	0.46 	0.29 	0.28 	0.33 	0.36 	0.25 	0.34 	0.26 	0.41 	0.39 	0.36 	0.39 	0.48 	0.39 	0.30 	0.37 	0.10 	0.23 
Glucose consumption rate	3.04 	3.14 	3.06 	2.73 	2.72 	2.84 	2.70 	2.76 	2.99 	2.70 	3.33 	2.82 	2.80 	3.00 	3.18 	3.36 	2.92 	3.63 	3.18 	4.15 	4.49 	2.97 	2.80 	2.94 	1.34 	5.05 	6.63 	13.34 
Oxygen uptake rate (OUR)	15.53 	5.44 	5.79 	4.45 	9.56 	5.83 	6.00 	8.08 	6.79 	6.71 	5.05 	7.17 	6.06 	9.95 	8.76 	11.87 	6.01 	8.04 	3.02 	15.52 	9.00 	12.21 	3.40 	10.99 	2.20 	9.57 	13.05 	14.87 
Carbon dioxide evolution rate (CER)	5.76 	6.46 	6.12 	5.04 	2.94 	3.84 	4.10 	2.40 	6.14 	4.81 	5.05 	5.03 	4.85 	4.11 	5.16 	7.09 	4.11 	6.32 	6.21 	6.99 	7.57 	5.95 	5.91 	6.81 	0.31 	7.88 	8.95 	10.83 
