ENTRY       zma03030                    Pathway
NAME        DNA replication - Zea mays (maize)
DESCRIPTION A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
CLASS       Genetic Information Processing; Replication and repair
PATHWAY_MAP zma03030  DNA replication
ORGANISM    Zea mays (maize) [GN:zma]
GENE        100285474  single-stranded DNA-binding protein [KO:K03111]
            103642101  DNA polymerase I A, chloroplastic [KO:K02335] [EC:2.7.7.7]
            103641202  DNA polymerase I A, chloroplastic isoform X1 [KO:K02335] [EC:2.7.7.7]
            100285427  uncharacterized protein LOC100285427 [KO:K07466]
            100383373  uncharacterized protein LOC100383373 [KO:K07466]
            100280726  uncharacterized protein LOC100280726 [KO:K07466]
            100273584  Replication protein A DNA-binding subunit [KO:K07466]
            542425  proliferating cell nuclear antigen [KO:K04802]
            542357  proliferating cell nuclear antigen 2 [KO:K04802]
            103648427  DNA replication ATP-dependent helicase/nuclease JHS1 isoform X1 [KO:K10742] [EC:5.6.2.3 3.1.-.-]
            732817  flap endonuclease 1 [KO:K04799] [EC:3.1.-.-]
            100383660  uncharacterized protein LOC100383660 [KO:K04799] [EC:3.1.-.-]
            100383890  uncharacterized protein LOC100383890 [KO:K10747] [EC:6.5.1.1 6.5.1.6 6.5.1.7]
            100194009  uncharacterized protein LOC100194009 [KO:K02320] [EC:2.7.7.7]
            100191506  uncharacterized protein LOC100191506 [KO:K02321]
            103631463  DNA primase small subunit [KO:K02684] [EC:2.7.7.102]
            100191752  uncharacterized protein LOC100191752 [KO:K02685]
            100384853  uncharacterized protein LOC100384853 [KO:K02685]
            103654581  DNA polymerase delta catalytic subunit [KO:K02327] [EC:2.7.7.7]
            100382167  uncharacterized protein LOC100382167 [KO:K02328]
            100194011  uncharacterized protein LOC100194011 [KO:K03504]
            100279971  uncharacterized protein LOC100279971 [KO:K03504]
            103642327  uncharacterized protein LOC103642327 [KO:K03504]
            100282638  uncharacterized protein LOC100282638 [KO:K03505]
            100280534  delta DNA polymerase [KO:K03505]
            103627197  DNA polymerase epsilon catalytic subunit A isoform X1 [KO:K02324] [EC:2.7.7.7]
            100280690  DNA polymerase epsilon subunit 2 [KO:K02325] [EC:2.7.7.7]
            100282897  uncharacterized protein LOC100282897 [KO:K02326] [EC:2.7.7.7]
            100501077  uncharacterized protein LOC100501077 [KO:K02540] [EC:5.6.2.3]
            542738  DNA replication licensing factor MCM3 homolog 2 [KO:K02541] [EC:5.6.2.3]
            100193296  putative mini-chromosome maintenance (MCM) complex protein family [KO:K02541] [EC:5.6.2.3]
            100281587  DNA replication licensing factor mcm4 [KO:K02212] [EC:5.6.2.3]
            100383139  uncharacterized protein LOC100383139 [KO:K02212] [EC:5.6.2.3]
            100304310  uncharacterized protein LOC100304310 [KO:K02209] [EC:5.6.2.3]
            100216891  DNA replication licensing factor MCM5 [KO:K02209] [EC:5.6.2.3]
            542206  minichromosome maintenance protein [KO:K02542] [EC:5.6.2.3]
            100279753  uncharacterized protein LOC100279753 [KO:K02542] [EC:5.6.2.3]
            542504  replication licensing factor MCM7 homologue [KO:K02210] [EC:5.6.2.3]
            100273978  uncharacterized protein LOC100273978 [KO:K02210] [EC:5.6.2.3]
            100285013  OB-fold nucleic acid binding domain containing protein [KO:K10739]
            100285903  OB-fold nucleic acid binding domain containing protein [KO:K10739]
            100272286  uncharacterized protein LOC100272286 [KO:K10739]
            103627452  replication protein A 32 kDa subunit B isoform X1 [KO:K10739]
            100283481  uncharacterized protein LOC100283481 [KO:K10740]
            100126965  uncharacterized protein LOC100126965 [KO:K10740]
            100285910  uncharacterized protein LOC100285910 [KO:K10740]
            103648088  replication factor C subunit 1 [KO:K10754]
            100282197  replication factor C subunit 2 [KO:K10755]
            100282678  uncharacterized protein LOC100282678 [KO:K10755]
            100216703  replication factor C subunit 3 [KO:K10756]
            100193357  uncharacterized protein LOC100193357 [KO:K10756]
            103625817  replication factor C subunit 5 [KO:K10756]
            103637697  replication factor C subunit 5 [KO:K10756]
            103640768  ribonuclease H2 subunit A [KO:K10743] [EC:3.1.26.4]
            100216620  uncharacterized protein LOC100216620 [KO:K10744]
            100275043  uncharacterized protein LOC100275043 [KO:K10745]
            103626668  uncharacterized protein LOC103626668 isoform X2 [KO:K10745]
REFERENCE   PMID:8087839
  AUTHORS   Stillman B.
  TITLE     Smart machines at the DNA replication fork.
  JOURNAL   Cell 78:725-8 (1994)
            DOI:10.1016/S0092-8674(94)90362-X
REFERENCE   PMID:9759502
  AUTHORS   Waga S, Stillman B.
  TITLE     The DNA replication fork in eukaryotic cells.
  JOURNAL   Annu Rev Biochem 67:721-51 (1998)
            DOI:10.1146/annurev.biochem.67.1.721
KO_PATHWAY  ko03030
///
