ENTRY       sly00510                    Pathway
NAME        N-Glycan biosynthesis - Solanum lycopersicum (tomato)
DESCRIPTION N-glycans or asparagine-linked glycans are major constituents of glycoproteins in eukaryotes. N-glycans are covalently attached to asparagine with the consensus sequence of Asn-X-Ser/Thr by an N-glycosidic bond, GlcNAc b1- Asn. Biosynthesis of N-glycans begins on the cytoplasmic face of the ER membrane with the transferase reaction of UDP-GlcNAc and the lipid-like precursor P-Dol (dolichol phosphate) to generate GlcNAc a1- PP-Dol. After sequential addition of monosaccharides by ALG glycosyltransferases [MD:M00055], the N-glycan precursor is attached by the OST (oligosaccharyltransferase) complex to the polypeptide chain that is being synthesized and translocated through the ER membrane. The protein-bound N-glycan precursor is subsequently trimmed, extended, and modified in the ER and Golgi by a complex series of reactions catalyzed by membrane-bound glycosidases and glycosyltransferases. N-glycans thus synthesized are classified into three types: high-mannose type, complex type, and hybrid type. Defects in N-glycan biosynthesis lead to a variety of human diseases known as congenital disorders of glycosylation [DS:H00118 H00119].
CLASS       Metabolism; Glycan biosynthesis and metabolism
PATHWAY_MAP sly00510  N-Glycan biosynthesis
MODULE      sly_M00055  N-glycan precursor biosynthesis [PATH:sly00510]
            sly_M00072  N-glycosylation by oligosaccharyltransferase [PATH:sly00510]
            sly_M00073  N-glycan precursor trimming [PATH:sly00510]
DBLINKS     GO: 0006487
ORGANISM    Solanum lycopersicum (tomato) [GN:sly]
GENE        101261171  polyprenol reductase 2 isoform X1 [KO:K12345] [EC:1.3.1.22 1.3.1.94]
            101266730  dolichol kinase EVAN isoform X1 [KO:K00902] [EC:2.7.1.108]
            101256171  UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase [KO:K01001] [EC:2.7.8.15]
            101264522  dolichyl-phosphate beta-glucosyltransferase [KO:K00729] [EC:2.4.1.117]
            101246510  UDP-N-acetylglucosamine transferase subunit ALG13 homolog [KO:K07432] [EC:2.4.1.141]
            101244457  UDP-N-acetylglucosamine transferase subunit ALG14-like protein [KO:K07441] [EC:2.4.1.141]
            101257856  dolichol-phosphate mannosyltransferase subunit 1 [KO:K00721] [EC:2.4.1.83]
            101255206  dolichol-phosphate mannose synthase subunit 2-like [KO:K09658]
            101243682  dolichol-phosphate mannose synthase subunit 2-like [KO:K09658]
            101264537  dolichol-phosphate mannose synthase subunit 2 [KO:K09658]
            101261146  dolichol-phosphate mannose synthase subunit 3 [KO:K09659]
            101244134  UDP-glycosyltransferase TURAN isoform X1 [KO:K03842] [EC:2.4.1.142]
            101245960  alpha-1,3/1,6-mannosyltransferase ALG2 [KO:K03843] [EC:2.4.1.132 2.4.1.257]
            101250940  GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase isoform X1 [KO:K03844] [EC:2.4.1.131]
            101251511  dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [KO:K03845] [EC:2.4.1.258]
            101267427  dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase [KO:K03846] [EC:2.4.1.259 2.4.1.261]
            101253423  dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase isoform X2 [KO:K03847] [EC:2.4.1.260]
            101245516  probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase isoform X1 [KO:K03848] [EC:2.4.1.267]
            101247658  probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase isoform X1 [KO:K03849] [EC:2.4.1.265]
            101252805  dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase isoform X1 [KO:K03850] [EC:2.4.1.256]
            101256652  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [KO:K07151] [EC:2.4.99.18]
            101259924  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B [KO:K07151] [EC:2.4.99.18]
            101264927  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1A [KO:K12666]
            101250991  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [KO:K12666]
            101265028  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 [KO:K12667]
            101252001  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 [KO:K12667]
            543753  DAD1; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 [KO:K12668]
            101256748  probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B [KO:K12669]
            101261758  probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B [KO:K12669]
            101261568  probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B [KO:K12669]
            101245405  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit precursor [KO:K12670]
            101252919  lipid phosphate phosphatase gamma [KO:K07252] [EC:3.6.1.43]
            101267114  mannosyl-oligosaccharide glucosidase GCS1 [KO:K01228] [EC:3.2.1.106]
            543798  gluII; alpha glucosidase II precursor [KO:K05546] [EC:3.2.1.207]
            101246787  mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [KO:K23741] [EC:3.2.1.209]
            101264908  mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1-like isoform X1 [KO:K01230] [EC:3.2.1.113]
            101262322  mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1-like isoform X1 [KO:K01230] [EC:3.2.1.113]
            101257812  alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase isoform X3 [KO:K00726] [EC:2.4.1.101]
            101266287  alpha-mannosidase 2 [KO:K01231] [EC:3.2.1.114]
            101259650  alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [KO:K00736] [EC:2.4.1.143]
            101260885  uncharacterized protein LOC101260885 isoform X1 [KO:K00737] [EC:2.4.1.144]
            101251860  uncharacterized protein LOC101251860 isoform X1 [KO:K00737] [EC:2.4.1.144]
            101268683  uncharacterized protein LOC101268683 [KO:K00737] [EC:2.4.1.144]
            101244199  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [KO:K00737] [EC:2.4.1.144]
COMPOUND    C00096  GDP-mannose
            C00110  Dolichyl phosphate
            C00381  Dolichol
            C00621  Dolichyl diphosphate
            C01246  Dolichyl beta-D-glucosyl phosphate
            C03021  Protein asparagine
            C03862  Dolichyl phosphate D-mannose
            C06081  Polyprenol
            G00001  N-Acetyl-D-glucosaminyldiphosphodolichol
            G00002  N,N'-Chitobiosyldiphosphodolichol
            G00003
            G00004
            G00005
            G00006
            G00007
            G00008
            G00009
            G00010
            G00011
            G00012
            G00013
            G00014
            G00015
            G00016
            G00017
            G00018  DS 3
            G00019
            G00020
            G00021
            G00022
            G00171
            G10526
            G10595
            G10596
            G10597
            G10598
            G10599
            G10694
REFERENCE   PMID:9111134
  AUTHORS   Schutzbach JS.
  TITLE     The role of the lipid matrix in the biosynthesis of dolichyl-linked oligosaccharides.
  JOURNAL   Glycoconj J 14:175-82 (1997)
            DOI:10.1023/A:1018533620002
REFERENCE   PMID:12200473
  AUTHORS   Oriol R, Martinez-Duncker I, Chantret I, Mollicone R, Codogno P.
  TITLE     Common origin and evolution of glycosyltransferases using Dol-P-monosaccharides as donor substrate.
  JOURNAL   Mol Biol Evol 19:1451-63 (2002)
            DOI:10.1093/oxfordjournals.molbev.a004208
REFERENCE   PMID:12684507
  AUTHORS   Thiel C, Schwarz M, Peng J, Grzmil M, Hasilik M, Braulke T, Kohlschutter A, von Figura K, Lehle L, Korner C.
  TITLE     A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis.
  JOURNAL   J Biol Chem 278:22498-505 (2003)
            DOI:10.1074/jbc.M302850200
REFERENCE   PMID:11278778
  AUTHORS   Cipollo JF, Trimble RB, Chi JH, Yan Q, Dean N.
  TITLE     The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum.
  JOURNAL   J Biol Chem 276:21828-40 (2001)
            DOI:10.1074/jbc.M010896200
REFERENCE   PMID:15987956
  AUTHORS   Frank CG, Aebi M.
  TITLE     ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis.
  JOURNAL   Glycobiology 15:1156-63 (2005)
            DOI:10.1093/glycob/cwj002
REFERENCE   PMID:16878994
  AUTHORS   O'Reilly MK, Zhang G, Imperiali B.
  TITLE     In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis.
  JOURNAL   Biochemistry 45:9593-603 (2006)
            DOI:10.1021/bi060878o
REL_PATHWAY sly00051  Fructose and mannose metabolism
            sly00511  Other glycan degradation
            sly00513  Various types of N-glycan biosynthesis
            sly00520  Amino sugar and nucleotide sugar metabolism
            sly00563  Glycosylphosphatidylinositol (GPI)-anchor biosynthesis
            sly00900  Terpenoid backbone biosynthesis
KO_PATHWAY  ko00510
///
