ENTRY       mtr03030                    Pathway
NAME        DNA replication - Medicago truncatula (barrel medic)
DESCRIPTION A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
CLASS       Genetic Information Processing; Replication and repair
PATHWAY_MAP mtr03030  DNA replication
ORGANISM    Medicago truncatula (barrel medic) [GN:mtr]
GENE        11446584  single-stranded DNA-binding protein, mitochondrial [KO:K03111]
            11444798  DNA polymerase I A, chloroplastic/mitochondrial isoform X1 [KO:K02335] [EC:2.7.7.7]
            11436067  DNA polymerase I A, chloroplastic/mitochondrial [KO:K02335] [EC:2.7.7.7]
            11444264  DNA polymerase I A, chloroplastic/mitochondrial isoform X2 [KO:K02335] [EC:2.7.7.7]
            25493890  replication protein A 70 kDa DNA-binding subunit B [KO:K07466]
            25486480  replication protein A 70 kDa DNA-binding subunit A [KO:K07466]
            25490757  proliferating cell nuclear antigen [KO:K04802]
            25498012  DNA replication ATP-dependent helicase/nuclease JHS1 isoform X1 [KO:K10742] [EC:5.6.2.3 3.1.-.-]
            11412823  flap endonuclease 1 [KO:K04799] [EC:3.1.-.-]
            25492370  DNA ligase 1 [KO:K10747] [EC:6.5.1.1 6.5.1.6 6.5.1.7]
            11410926  DNA polymerase alpha catalytic subunit [KO:K02320] [EC:2.7.7.7]
            11441066  DNA polymerase alpha subunit B [KO:K02321]
            11439115  DNA primase small subunit [KO:K02684] [EC:2.7.7.102]
            11439551  probable DNA primase large subunit [KO:K02685]
            25489857  DNA polymerase delta catalytic subunit [KO:K02327] [EC:2.7.7.7]
            11437014  DNA polymerase delta small subunit [KO:K02328]
            11408397  DNA polymerase delta subunit 3 isoform X1 [KO:K03504]
            25493347  DNA polymerase delta subunit 4 [KO:K03505]
            25493349  DNA polymerase delta subunit 4 [KO:K03505]
            25485289  DNA polymerase delta subunit 4 [KO:K03505]
            11445193  DNA polymerase epsilon catalytic subunit A isoform X1 [KO:K02324] [EC:2.7.7.7]
            25496685  DNA polymerase epsilon subunit B [KO:K02325] [EC:2.7.7.7]
            25492285  DNA polymerase epsilon subunit 3 [KO:K02326] [EC:2.7.7.7]
            11413210  DNA replication licensing factor MCM2 [KO:K02540] [EC:5.6.2.3]
            11407765  DNA replication licensing factor MCM3 [KO:K02541] [EC:5.6.2.3]
            11440570  DNA replication licensing factor MCM4 [KO:K02212] [EC:5.6.2.3]
            25493974  DNA replication licensing factor MCM5 [KO:K02209] [EC:5.6.2.3]
            25501880  DNA replication licensing factor MCM6 [KO:K02542] [EC:5.6.2.3]
            25488187  DNA replication licensing factor MCM6 [KO:K02542] [EC:5.6.2.3]
            11430699  DNA replication licensing factor MCM7 [KO:K02210] [EC:5.6.2.3]
            11446020  replication protein A 32 kDa subunit B [KO:K10739]
            25481878  replication protein A 32 kDa subunit A [KO:K10739]
            11433064  replication protein A 14 kDa subunit B [KO:K10740]
            25485573  replication protein A 14 kDa subunit B [KO:K10740]
            25485575  replication protein A 14 kDa subunit B [KO:K10740]
            25492173  replication factor C subunit 1 isoform X1 [KO:K10754]
            11414806  replication factor C subunit 2 [KO:K10755]
            25493662  replication factor C subunit 2 [KO:K10755]
            11414029  replication factor C subunit 3 [KO:K10756]
            11429308  uncharacterized protein LOC11429308 [KO:K10756]
            25495823  replication factor C subunit 3 [KO:K10756]
            25497284  replication factor C subunit 3 [KO:K10756]
            120575764  uncharacterized protein LOC120575764 [KO:K10756]
            25485570  ribonuclease H2 subunit A isoform X1 [KO:K10743] [EC:3.1.26.4]
            112420388  ribonuclease H2 subunit B [KO:K10744]
REFERENCE   PMID:8087839
  AUTHORS   Stillman B.
  TITLE     Smart machines at the DNA replication fork.
  JOURNAL   Cell 78:725-8 (1994)
            DOI:10.1016/S0092-8674(94)90362-X
REFERENCE   PMID:9759502
  AUTHORS   Waga S, Stillman B.
  TITLE     The DNA replication fork in eukaryotic cells.
  JOURNAL   Annu Rev Biochem 67:721-51 (1998)
            DOI:10.1146/annurev.biochem.67.1.721
KO_PATHWAY  ko03030
///
