ENTRY       gmx03030                    Pathway
NAME        DNA replication - Glycine max (soybean)
DESCRIPTION A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
CLASS       Genetic Information Processing; Replication and repair
PATHWAY_MAP gmx03030  DNA replication
ORGANISM    Glycine max (soybean) [GN:gmx]
GENE        100809336  single-stranded DNA-binding protein, mitochondrial [KO:K03111]
            100797016  DNA polymerase I A, chloroplastic/mitochondrial [KO:K02335] [EC:2.7.7.7]
            100807239  DNA polymerase I A, chloroplastic/mitochondrial [KO:K02335] [EC:2.7.7.7]
            100790704  DNA polymerase I B, chloroplastic/mitochondrial isoform X1 [KO:K02335] [EC:2.7.7.7]
            100778597  replication protein A 70 kDa DNA-binding subunit A [KO:K07466]
            100792409  replication protein A 70 kDa DNA-binding subunit A [KO:K07466]
            100777091  replication protein A 70 kDa DNA-binding subunit B [KO:K07466]
            548079  proliferating cell nuclear antigen [KO:K04802]
            100817651  proliferating cell nuclear antigen [KO:K04802]
            100780038  proliferating cell nuclear antigen [KO:K04802]
            100799415  DNA replication ATP-dependent helicase/nuclease JHS1 [KO:K10742] [EC:5.6.2.3 3.1.-.-]
            100808044  flap endonuclease 1 [KO:K04799] [EC:3.1.-.-]
            100792878  flap endonuclease 1-like [KO:K04799] [EC:3.1.-.-]
            100783155  DNA ligase 1 [KO:K10747] [EC:6.5.1.1 6.5.1.6 6.5.1.7]
            100798779  DNA polymerase alpha catalytic subunit [KO:K02320] [EC:2.7.7.7]
            100795306  DNA polymerase alpha subunit B isoform X1 [KO:K02321]
            100797769  DNA polymerase alpha subunit B [KO:K02321]
            100780574  DNA primase small subunit [KO:K02684] [EC:2.7.7.102]
            100806321  probable DNA primase large subunit [KO:K02685]
            100816023  probable DNA primase large subunit isoform X1 [KO:K02685]
            548092  POLD1; DNA polymerase delta catalytic subunit [KO:K02327] [EC:2.7.7.7]
            100817379  DNA polymerase delta catalytic subunit [KO:K02327] [EC:2.7.7.7]
            100780072  DNA polymerase delta small subunit [KO:K02328]
            100805951  DNA polymerase delta small subunit isoform X1 [KO:K02328]
            100812372  DNA polymerase delta subunit 3 [KO:K03504]
            100793247  uncharacterized protein LOC100793247 isoform X1 [KO:K03504]
            100778329  DNA polymerase delta subunit 4 [KO:K03505]
            100799459  DNA polymerase delta subunit 4 [KO:K03505]
            100812003  DNA polymerase epsilon catalytic subunit A [KO:K02324] [EC:2.7.7.7]
            100778115  LOW QUALITY PROTEIN: DNA polymerase epsilon catalytic subunit A-like [KO:K02324] [EC:2.7.7.7]
            100784116  DNA polymerase epsilon subunit B isoform X2 [KO:K02325] [EC:2.7.7.7]
            100819488  DNA polymerase epsilon subunit 3 [KO:K02326] [EC:2.7.7.7]
            100306382  uncharacterized protein LOC100306382 [KO:K02326] [EC:2.7.7.7]
            100799441  DNA replication licensing factor MCM2 [KO:K02540] [EC:5.6.2.3]
            100813006  DNA replication licensing factor MCM2 [KO:K02540] [EC:5.6.2.3]
            100791302  DNA replication licensing factor MCM3 homolog 2 [KO:K02541] [EC:5.6.2.3]
            100811545  DNA replication licensing factor MCM3 [KO:K02541] [EC:5.6.2.3]
            100782586  DNA replication licensing factor MCM4 [KO:K02212] [EC:5.6.2.3]
            100805788  DNA replication licensing factor MCM4 [KO:K02212] [EC:5.6.2.3]
            100800649  DNA replication licensing factor MCM5 [KO:K02209] [EC:5.6.2.3]
            100779483  DNA replication licensing factor MCM5 [KO:K02209] [EC:5.6.2.3]
            100794021  DNA replication licensing factor MCM6 [KO:K02542] [EC:5.6.2.3]
            100787290  DNA replication licensing factor MCM6 isoform X1 [KO:K02542] [EC:5.6.2.3]
            100802903  DNA replication licensing factor MCM7 [KO:K02210] [EC:5.6.2.3]
            100811639  DNA replication licensing factor MCM7 [KO:K02210] [EC:5.6.2.3]
            100789756  replication protein A 32 kDa subunit A [KO:K10739]
            100782819  replication protein A 32 kDa subunit B [KO:K10739]
            100802512  replication protein A 32 kDa subunit A [KO:K10739]
            100306197  uncharacterized protein LOC100306197 [KO:K10740]
            100306641  uncharacterized protein LOC100306641 [KO:K10740]
            100781484  replication protein A 14 kDa subunit B [KO:K10740]
            100802809  replication factor C subunit 1 [KO:K10754]
            100790070  replication factor C subunit 1 isoform X1 [KO:K10754]
            100788329  replication factor C subunit 2 isoform X1 [KO:K10755]
            100781364  replication factor C subunit 2 [KO:K10755]
            100798331  replication factor C subunit 2 [KO:K10755]
            100775926  replication factor C subunit 2 [KO:K10755]
            100818329  uncharacterized protein LOC100818329 [KO:K10756]
            100782652  replication factor C subunit 3 [KO:K10756]
            100807039  replication factor C subunit 3 isoform X1 [KO:K10756]
            100805231  replication factor C subunit 3 [KO:K10756]
            100817775  uncharacterized protein LOC100817775 isoform X2 [KO:K10756]
            102664389  uncharacterized protein LOC102664389 [KO:K10756]
            100795321  uncharacterized protein LOC100795321 [KO:K10756]
            100779875  ribonuclease H2 subunit A [KO:K10743] [EC:3.1.26.4]
            100305835  ribonuclease H2-B superfamily protein [KO:K10744]
            100792495  ribonuclease H2 subunit B [KO:K10744]
            100500515  uncharacterized protein LOC100500515 [KO:K10745]
            100785195  uncharacterized protein LOC100785195 [KO:K10745]
REFERENCE   PMID:8087839
  AUTHORS   Stillman B.
  TITLE     Smart machines at the DNA replication fork.
  JOURNAL   Cell 78:725-8 (1994)
            DOI:10.1016/S0092-8674(94)90362-X
REFERENCE   PMID:9759502
  AUTHORS   Waga S, Stillman B.
  TITLE     The DNA replication fork in eukaryotic cells.
  JOURNAL   Annu Rev Biochem 67:721-51 (1998)
            DOI:10.1146/annurev.biochem.67.1.721
KO_PATHWAY  ko03030
///
