ENTRY       gmx00510                    Pathway
NAME        N-Glycan biosynthesis - Glycine max (soybean)
DESCRIPTION N-glycans or asparagine-linked glycans are major constituents of glycoproteins in eukaryotes. N-glycans are covalently attached to asparagine with the consensus sequence of Asn-X-Ser/Thr by an N-glycosidic bond, GlcNAc b1- Asn. Biosynthesis of N-glycans begins on the cytoplasmic face of the ER membrane with the transferase reaction of UDP-GlcNAc and the lipid-like precursor P-Dol (dolichol phosphate) to generate GlcNAc a1- PP-Dol. After sequential addition of monosaccharides by ALG glycosyltransferases [MD:M00055], the N-glycan precursor is attached by the OST (oligosaccharyltransferase) complex to the polypeptide chain that is being synthesized and translocated through the ER membrane. The protein-bound N-glycan precursor is subsequently trimmed, extended, and modified in the ER and Golgi by a complex series of reactions catalyzed by membrane-bound glycosidases and glycosyltransferases. N-glycans thus synthesized are classified into three types: high-mannose type, complex type, and hybrid type. Defects in N-glycan biosynthesis lead to a variety of human diseases known as congenital disorders of glycosylation [DS:H00118 H00119].
CLASS       Metabolism; Glycan biosynthesis and metabolism
PATHWAY_MAP gmx00510  N-Glycan biosynthesis
MODULE      gmx_M00055  N-glycan precursor biosynthesis [PATH:gmx00510]
            gmx_M00072  N-glycosylation by oligosaccharyltransferase [PATH:gmx00510]
            gmx_M00073  N-glycan precursor trimming [PATH:gmx00510]
DBLINKS     GO: 0006487
ORGANISM    Glycine max (soybean) [GN:gmx]
GENE        100814660  polyprenol reductase 2 [KO:K12345] [EC:1.3.1.22 1.3.1.94]
            100793756  dolichol kinase EVAN isoform X1 [KO:K00902] [EC:2.7.1.108]
            100799242  dolichol kinase EVAN isoform X1 [KO:K00902] [EC:2.7.1.108]
            100801463  uncharacterized protein LOC100801463 [KO:K01001] [EC:2.7.8.15]
            100789475  UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase [KO:K01001] [EC:2.7.8.15]
            100781283  dolichyl-phosphate beta-glucosyltransferase isoform X1 [KO:K00729] [EC:2.4.1.117]
            100793179  dolichyl-phosphate beta-glucosyltransferase [KO:K00729] [EC:2.4.1.117]
            100526989  glycosyltransferase family protein [KO:K07432] [EC:2.4.1.141]
            100527305  uncharacterized protein LOC100527305 [KO:K07432] [EC:2.4.1.141]
            100775914  UDP-N-acetylglucosamine transferase subunit ALG14 homolog isoform X2 [KO:K07441] [EC:2.4.1.141]
            100778556  UDP-N-acetylglucosamine transferase subunit ALG14 isoform X2 [KO:K07441] [EC:2.4.1.141]
            102664592  uncharacterized protein LOC102664592 [KO:K07441] [EC:2.4.1.141]
            100811229  UDP-N-acetylglucosamine transferase subunit ALG14 homolog isoform X1 [KO:K07441] [EC:2.4.1.141]
            100500321  putative dolichol phosphate mannase synthase [KO:K00721] [EC:2.4.1.83]
            100802117  dolichol-phosphate mannosyltransferase subunit 1 [KO:K00721] [EC:2.4.1.83]
            100527352  dolichol-phosphate mannose synthase subunit 2 [KO:K09658]
            100786362  dolichol-phosphate mannose synthase subunit 2 [KO:K09658]
            100775904  dolichol-phosphate mannose synthase subunit 3 [KO:K09659]
            100796507  dolichol-phosphate mannose synthase subunit 3 [KO:K09659]
            100789238  UDP-glycosyltransferase TURAN isoform X1 [KO:K03842] [EC:2.4.1.142]
            100809168  uncharacterized protein LOC100809168 [KO:K03843] [EC:2.4.1.132 2.4.1.257]
            100800839  alpha-1,3/1,6-mannosyltransferase ALG2 isoform X1 [KO:K03843] [EC:2.4.1.132 2.4.1.257]
            100808662  GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase [KO:K03844] [EC:2.4.1.131]
            100801617  GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase [KO:K03844] [EC:2.4.1.131]
            100785253  dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [KO:K03845] [EC:2.4.1.258]
            100793743  dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [KO:K03845] [EC:2.4.1.258]
            100805971  dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase isoform X1 [KO:K03846] [EC:2.4.1.259 2.4.1.261]
            100805094  dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase [KO:K03846] [EC:2.4.1.259 2.4.1.261]
            100786127  dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase isoform X1 [KO:K03847] [EC:2.4.1.260]
            100776070  dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase isoform X2 [KO:K03847] [EC:2.4.1.260]
            100809881  probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase [KO:K03848] [EC:2.4.1.267]
            100802149  probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase [KO:K03849] [EC:2.4.1.265]
            100792228  dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [KO:K03850] [EC:2.4.1.256]
            100792293  dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase isoform X1 [KO:K03850] [EC:2.4.1.256]
            100805873  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [KO:K07151] [EC:2.4.99.18]
            100780916  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B [KO:K07151] [EC:2.4.99.18]
            100782502  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [KO:K07151] [EC:2.4.99.18]
            100816018  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B [KO:K07151] [EC:2.4.99.18]
            100817203  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1A [KO:K12666]
            100814638  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [KO:K12666]
            100796192  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1A [KO:K12666]
            100817179  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [KO:K12666]
            100780415  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 [KO:K12667]
            100814831  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 [KO:K12667]
            100806815  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 [KO:K12667]
            100306016  oligosaccharyl transferase subunit DAD1-like protein isoform 2 [KO:K12668]
            100814967  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 [KO:K12668]
            100499801  DAD1; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 [KO:K12668]
            100789480  probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B [KO:K12669]
            100793228  oligosaccharide transmembrane transporter precursor [KO:K12669]
            100806848  dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit [KO:K12670]
            100806625  lipid phosphate phosphatase gamma [KO:K07252] [EC:3.6.1.43]
            100783896  lipid phosphate phosphatase gamma [KO:K07252] [EC:3.6.1.43]
            100795161  mannosyl-oligosaccharide glucosidase GCS1 [KO:K01228] [EC:3.2.1.106]
            100795691  mannosyl-oligosaccharide glucosidase GCS1 [KO:K01228] [EC:3.2.1.106]
            100817168  probable glucan 1,3-alpha-glucosidase [KO:K05546] [EC:3.2.1.207]
            100789211  mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [KO:K23741] [EC:3.2.1.209]
            100806551  mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [KO:K23741] [EC:3.2.1.209]
            547540  MAN1; mannosyl-oligosaccharide 1,2-alpha-mannosidase [KO:K01230] [EC:3.2.1.113]
            100819189  mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1 [KO:K01230] [EC:3.2.1.113]
            100816571  mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1 [KO:K01230] [EC:3.2.1.113]
            100776378  alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [KO:K00726] [EC:2.4.1.101]
            100795005  alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase isoform X1 [KO:K00726] [EC:2.4.1.101]
            100806647  alpha-mannosidase 2 [KO:K01231] [EC:3.2.1.114]
            100811858  alpha-mannosidase 2 isoform X1 [KO:K01231] [EC:3.2.1.114]
            100819421  alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [KO:K00736] [EC:2.4.1.143]
            100787062  uncharacterized protein LOC100787062 [KO:K00737] [EC:2.4.1.144]
            100780085  uncharacterized protein LOC100780085 [KO:K00737] [EC:2.4.1.144]
            100811451  uncharacterized protein LOC100811451 isoform X1 [KO:K00737] [EC:2.4.1.144]
            100805850  putative beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [KO:K00737] [EC:2.4.1.144]
            100807933  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [KO:K00737] [EC:2.4.1.144]
            100804627  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [KO:K00737] [EC:2.4.1.144]
            100778832  uncharacterized protein LOC100778832 [KO:K00737] [EC:2.4.1.144]
            100816069  uncharacterized protein LOC100816069 [KO:K00737] [EC:2.4.1.144]
COMPOUND    C00096  GDP-mannose
            C00110  Dolichyl phosphate
            C00381  Dolichol
            C00621  Dolichyl diphosphate
            C01246  Dolichyl beta-D-glucosyl phosphate
            C03021  Protein asparagine
            C03862  Dolichyl phosphate D-mannose
            C06081  Polyprenol
            G00001  N-Acetyl-D-glucosaminyldiphosphodolichol
            G00002  N,N'-Chitobiosyldiphosphodolichol
            G00003
            G00004
            G00005
            G00006
            G00007
            G00008
            G00009
            G00010
            G00011
            G00012
            G00013
            G00014
            G00015
            G00016
            G00017
            G00018  DS 3
            G00019
            G00020
            G00021
            G00022
            G00171
            G10526
            G10595
            G10596
            G10597
            G10598
            G10599
            G10694
REFERENCE   PMID:9111134
  AUTHORS   Schutzbach JS.
  TITLE     The role of the lipid matrix in the biosynthesis of dolichyl-linked oligosaccharides.
  JOURNAL   Glycoconj J 14:175-82 (1997)
            DOI:10.1023/A:1018533620002
REFERENCE   PMID:12200473
  AUTHORS   Oriol R, Martinez-Duncker I, Chantret I, Mollicone R, Codogno P.
  TITLE     Common origin and evolution of glycosyltransferases using Dol-P-monosaccharides as donor substrate.
  JOURNAL   Mol Biol Evol 19:1451-63 (2002)
            DOI:10.1093/oxfordjournals.molbev.a004208
REFERENCE   PMID:12684507
  AUTHORS   Thiel C, Schwarz M, Peng J, Grzmil M, Hasilik M, Braulke T, Kohlschutter A, von Figura K, Lehle L, Korner C.
  TITLE     A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis.
  JOURNAL   J Biol Chem 278:22498-505 (2003)
            DOI:10.1074/jbc.M302850200
REFERENCE   PMID:11278778
  AUTHORS   Cipollo JF, Trimble RB, Chi JH, Yan Q, Dean N.
  TITLE     The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum.
  JOURNAL   J Biol Chem 276:21828-40 (2001)
            DOI:10.1074/jbc.M010896200
REFERENCE   PMID:15987956
  AUTHORS   Frank CG, Aebi M.
  TITLE     ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis.
  JOURNAL   Glycobiology 15:1156-63 (2005)
            DOI:10.1093/glycob/cwj002
REFERENCE   PMID:16878994
  AUTHORS   O'Reilly MK, Zhang G, Imperiali B.
  TITLE     In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis.
  JOURNAL   Biochemistry 45:9593-603 (2006)
            DOI:10.1021/bi060878o
REL_PATHWAY gmx00051  Fructose and mannose metabolism
            gmx00511  Other glycan degradation
            gmx00513  Various types of N-glycan biosynthesis
            gmx00520  Amino sugar and nucleotide sugar metabolism
            gmx00563  Glycosylphosphatidylinositol (GPI)-anchor biosynthesis
            gmx00900  Terpenoid backbone biosynthesis
KO_PATHWAY  ko00510
///
