Specimens examined.—Specimens of northeastern P. maniculatus

were live trapped from the following localities (Texas Cooperative Wildlife Collection (TCWC) accession and GenBank numbers in parentheses): CANADA: Ontario; 10 km N Moonbeam (n =3), 49.3432°N, 82.1541°W (56252 – 56254, MK122967); Quebec; 11.5 mi E of Havre-Saint-Pierre (n =3), 50.2418°N, 63.5986°W (59869, 59870, 59872, MK122965 ̅ MK122967). USA: Vermont; Washington Co., New Discovery Campground (n =10), 44.1987°N, 72.6973°W (56413,56415 ̅ 56423, MK122965 ̅ MK122967, MK122971); Maine; Aroostook Co., Aroostook State Park (n =12), 46.6155°N, 68.0084°W (56385, 56398 ̅ 56408, MK122966, MK122968 ̅ MK122970); Hancock Co., Mount Desert Island (n =1), 44.3924°N, 68.3021°W (56410, MK122970). Specimens representing central P. maniculatus (n =20) were live-trapped from 1 mi S, 2.3 mi W of Hayes, Ellis Co, Kansas (56222, 56223, 56229 ̅ 56232, 56234 ̅ 56237, 56260, 56261, 56272 ̅ 56279, 56328, and MK122972 ̅ MK122978). The capture and handling of animals followed the recommendations of Sikes et al. (2016).

Reference sequences were obtained from GenBank as follows: central/western P. maniculatus, Colorado, Gilpin Co. (U40250); California, Kern Co. (KC764393) and Humboldt Co. (KC764395); Oregon, Benton Co. (KC764399) and Harney Co. (KC764400); Washington, Gray’s Harbor Co. (U40249) and Okanogan Co. (KC764408);