I. General information
Dataset title: eDNA quantification for bullfrog management

Principle investigator: Teun Everts (teun.everts@inbo.be)
Co-investigators: Charlotte Van Driessche, Sabrina Neyrinck, Nico De Regge, Sarah Descamps, Alain De Vocht, Hans Jacquemyn, Rein Brys

Data collection data: Betwee June and September 2021
Geographic location of data collection: Belgium, Flanders (Northern Region), Province of Antwerp.

Keywords: Alien invasive species; conservation genetics; depletion sampling; droplet digital PCR; environmental DNA quantification; fyke netting; Lithobates catesbeianus; monitoring biological invasions

Principal funders: Research Foundation Flanders grants for strategic basic research (FWO-SB, grant number 1S01822N to TE), the 3n-Bullfrog project (LIFE18 NAT/BE/001016), and the Research Institute for Nature and Forest


II. Data overview and methodological information
Datafile: EvertsEtAl2022_DS.xlsx
Creation data data file: the 25th of March 2022

The dataset contains five tab pages.
- 1. "Overview_Experimental_Ponds" gives an overview of the data collected for the experimental ponds. It gives the ID's for each pond, the coordinates, the circumference and area, the quantified water volume, the inroduced
abundances, and whether or not abiotic measurements have been carried out (1= measurements done, 0= no measruements done). Additionally, it shows how many individuals were introduced in each pond at what time period, when 
the eDNA sample was taken, and how many eDNA samples were taken. Finally, it shows how many total eDNA samples were taken from these experimental ponds.

- 2. "Overview_Management_Ponds" gives an overview of the data collected for the management ponds. It gives the ID's for each pond, the coordinates, the circumference and area, the quantified water volume, the number of eDNA
samples that were taken before ("#_Pre_Fyke_Samples") and after ("#_Post_Fyke_Samples") introduction of the fykes, how many time periods a pond was subject to eDNA-sampling and subsequent fyke netting ("#Independent_Fykenetting_Actions"),
how many independent conventional abundance estimates were calculated for each pond ("#_Conventional_Abundance_Estimates"), and whether or not abiotic measurements have been carried out (1= measurements done, 0= no measruements done).
Below, in row 33, it shows the total number for each column.

- 3. "Catches_ManagementPonds" gives an onverview of the bullfrog catch data for the management ponds, chronologically ordered. It gives the ID for each ponds, the date that the fykes were introduced ("Catch_Start"), the data that the
fykes were removed ("Catch_Stop"), the total number of days that bullfrogs were captured ("Number_Catch_Days"), the number of double fyke nets that were used ("Number_Double_Fykes"), the total number of bullfrogs that were captured,
regardeless of life stage ("Caught_Catch"), the total number of bullfrogs tadpoles that were captured ("Caught_Tadpoles"), the total number of bullfrogs juvenilesthat were captured ("Caught_Juveniles"), the total number of bullfrogs 
adults that were captured ("Caught_Adult"), the catch-per-unit-effort regardless of life stage ("Total_CPUE"), and multiple columns with the daily catch data ("Daily Catches): column K is the total bullfrog catch on day 1, column L is
the total bullfrog catch on day 2, etc.

- 4. "eDNA_Concentrations" gives an overview of all data related to the eDNA results for both experimental as management ponds. It gives the pond ID, the code of the corresponding eDNA filter, the number of introduced tadpoles (only relevant
for experimental ponds, hence the "-" for management ponds", the pond type (i.e. experimental or management ponds), the volume of pond water that was filtered over an eDNA filter (in mL), the volume of lysis that was retained from the
eDNA filter (in µL), the volume in which the final eDNA extract was eluted to (in µL), the dilution factor (which is set to two if the DNA extract was diluted in a 1:2 way to limited PCR-inhibiting compounds, and set to 1 if no
dilution was carried out), a powerclean correction factor (which is set to 1.5 if a powerclean process was carried out in the DNA extract, because this process strats from 150 µL and ends in 100 µL), the total number of accepted droplets
in ddPCR, the number of accepted droplets that were positive for the amplified bullfrog amplicon, the mean amplitude of the positive accepted dropets, the concentration of bullfrog eDNA in the ddPCR reaction volume ("Conc_(copies/µL)"),
the final bullfrog eDNA concentration according to the formulate that is presented in the Research Article (not corrected for a spatial metric), and the mean and standard error of the technical replicates of each sample.

- 5. "Abiotics" gives an overview of all abiotic measurements carried out both for experimental and management ponds.


III. Sharing access and information
This datasetis licensed under a Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the
original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.

This data was used generated in the Resarch Article:
Everts, T., Van Driessche, C., Neyrinck, S., De Regge, N., Descamps, S., De Vocht, A., Jacquemyn, H., Brys, R. (2022) Using quantitative eDNA analyses to accurately estimate American bullfrog abundance and to evaluate management efficacy.
Environmental DNA.

This work builds upon (i.e. primer/probe assay validation and ddPCR protocol):
Everts, T., Halfmaerten, D., Neyrinck, S., De Regge, N., Jacquemyn, H., Brys, R. (2021) Accurate detection and quantification of seasonal abundance of American bullfrog (Lithobates catesbeianus) using ddPCR eDNA assays. Scientific Reports
11:11282. https://doi.org/10.1038/s41598-021-90771-w



