﻿This README_Kauzalova-etal_2022.txt file was generated on 2022-01-18 by Tereza Kauzálová


GENERAL INFORMATION

1. Title of Dataset: Data for: Telomere length is highly repeatable and shorter in individuals with more elaborate sexual ornamentation in a short-lived passerine

2. Author Information
	A. Principal Investigator Contact Information
		Name: Tomáš Albrecht
		Institution: Institute of Vertebrate Biology, Czech Academy of Sciences
		Address: Květná 8, 603 65 Brno, Czech Republic
		Email: albrecht@ivb.cz

	B. Associate or Co-investigator Contact Information
		Name: Simon Verhulst
		Institution: Groningen Institute for Evolutionary Life Sciences, University of Groningen
		Address: PO Box 11103, 9700 CC Groningen, the Netherlands
		Email: s.verhulst@rug.nl

	C. Alternate Contact Information
		Name: Tereza Kauzálová
		Institution: Institute of Vertebrate Biology, Czech Academy of Sciences
		Address: Květná 8, 603 65 Brno, Czech Republic
		Email: terezk87@gmail.com

3. Date of data collection (single date, range, approximate date): years 2012-2017

4. Geographic location of data collection: Třeboň, Czech Republic (49.0077N, 14.7590E)

5. Information about funding sources that supported the collection of the data: Czech Science Foundation through projects GA19-22538S and GA21-22160S and one author was financially supported by Erasmus+


SHARING/ACCESS INFORMATION

1. Licenses/restrictions placed on the data: 

2. Links to publications that cite or use the data: preprint - https://www.authorea.com/users/415127/articles/523039-telomere-length-is-highly-repeatable-and-declines-with-more-elaborate-sexual-ornamentation-in-a-short-lived-passerine?commit=b996fbded893d5060d06efa8f5fa1e24dd105398

3. Links to other publicly accessible locations of the data: 

4. Links/relationships to ancillary data sets: 

5. Was data derived from another source? no
	A. If yes, list source(s): 

6. Recommended citation for this dataset: 


DATA & FILE OVERVIEW

1. File List: 	Kauzalova-etal_2022_main-data.xlsx - contains the main data used in the associated manuscript
		Kauzalova-etal_2022_inter-assay_rpt.xlsx - contains data for the calculation of the inter-assay repeatability of telomere length measurements

2. Relationship between files, if important: 

3. Additional related data collected that was not included in the current data package: 

4. Are there multiple versions of the dataset? no
	A. If yes, name of file(s) that was updated: 
		i. Why was the file updated? 
		ii. When was the file updated? 


METHODOLOGICAL INFORMATION

1. Description of methods used for collection/generation of data: Telomere length (TL) was measured using terminal restriction fragment (TRF) analysis according to Salomons et al. (2009). Telomere shortening and survival in free-living corvids. Proceedings. Biological Sciences / The Royal Society, 276(1670), 3157–3165. https://doi.org/10.1098/rspb.2009.0517

2. Methods for processing the data: The average TL for each sample was calculated from the sample-specific TL distribution using densitometry in program ImageJ2.

3. Instrument- or software-specific information needed to interpret the data: We used the statistical software R (ver. 4.0.5, R Core Team, 2020) running in the RStudio IDE (ver. 1.4.1106; RStudioTeam, 2020), packages rptR, lme4, lmerTest, survival, misty

4. Standards and calibration information, if appropriate: 

5. Environmental/experimental conditions: 

6. Describe any quality-assurance procedures performed on the data: 

7. People involved with sample collection, processing, analysis and/or submission: Tereza Kauzálová, Oldřich Tomášek, Ellis Mulder, Simon Verhulst, Tomáš Albrecht


DATA-SPECIFIC INFORMATION FOR: Kauzalova-etal_2022_main-data.xlsx

1. Number of variables: 23

2. Number of cases/rows: 278

3. Variable List:
Ring no. = ID of the individual, number of unique ornithological metal ring
Capture year = a year when the individual was captured and sampled
Capture date = exact date of individual sampling
Delta days = number of days between two captures of the same individual (units: days)
Source = blood sample type (rbc = red blood cells, blood = whole blood)
Sex = sex of the individual (M = male, F = female)
Birth year = a year when the individual was born
Age = age of the individual at the sampling
Age dif. = number of years between two sampling events of the same individual (units: years)
Mean age = the average age of the individual based on the two sampling events per individual (units: years)
Delta age = the deviance of actual age of the individual from its mean age (units: years)
Measurement = first (=1) or second (=2) measurement of the same individual
Mortality = information about the individual survival (0 = bird survived to the consecutive breeding season, 1 = bird presumably died)
Gel = number of gel analysed by TRF
Telomere length (TL) = mean telomere length of the specific sample (units: base pairs)
Change in TL = the difference in TL between the two measurements of the same individual
Breeding = breeding status of the individual (1 = the bird had a nest, 0 = the bird did not breed in this breeding season)
Tail length = the maximum length of the outermost tail feathers (units: mm)
Initial tail length = the tail length from the first sampling of the individual (units: mm)
Change in tail length = the difference between tail length from the second and first sampling event of the same individual (units: mm)
Mean tail length = the average tail length calculated from the two measurements of the same individual
Delta tail length = the deviance of actual tail length from the mean tail length of the individual
Tail intact = the information about the measured tail feather (yes = the feather was complete, no = the feather was broken)

4. Missing data codes: NA = not available

5. Specialized formats or other abbreviations used: no. = number, dif. = difference, TL = telomere length

DATA-SPECIFIC INFORMATION FOR: Kauzalova-etal_2022_inter-assay_rpt.xlsx

1. Number of variables: 3

2. Number of cases/rows: 37

3. Variable List: 
ID = identifier of the sample repeatedly measured on different TRF gels (chicken = chicken blood sample, samples beginning with S = barn swallow samples encoded with ring number + capture year)
Gel = number of gel analysed by TRF
Telomere length = mean telomere length of the specific sample (units: base pairs)

4. Missing data codes: none

5. Specialized formats or other abbreviations used: none

